NLRP3/Caspase-1通路对半夏厚朴汤干预慢性间歇性低氧小鼠肾脏炎性损伤中的作用*

doi:10.12047/j.cjap.6250.2022.055

摘要/Abstract

摘要： 目的: 观察半夏厚朴汤对慢性间歇性低氧小鼠肾脏NLRP3/Caspase-1/IL-1β信号通路的影响。方法: 将C57BL/6小鼠随机分为3组,每组10只,分别是正常对照组(Control)、慢性间歇性低氧组(CIH)、半夏厚朴汤治疗组(BHD)。CIH组与BHD组小鼠置于低氧舱内,先充入氮气,使氧气浓度在90 s内从21%下降到9%,后充入氧气,使氧气浓度在90 s内逐渐上升到21%,Control组小鼠置于舱内,充入正常空气,每天处理8 h,持续21 d。BHD组于每日入舱前灌胃给予半夏厚朴汤,Control组和CIH组同时灌胃等体积的生理盐水。造模结束检测各组小鼠肾功能变化和观察肾脏病理情况;通过蛋白印迹法和免疫组织化学染色法检测NOD样受体3(NLRP3)、天冬氨酸特异性半胱氨酸蛋白1(Caspase-1)、白介素 (IL-1β) 的表达水平。结果: 与Control组比较,CIH组小鼠血清尿酸(UA)、尿素氮(BUN)及肌酐(SCr)的含量显著升高(P<0.01),与CIH组比较,BHD组均显著下降(P<0.01)。病理染色结果显示,与对照组相比,CIH组肾小管管腔增大,肾小管上皮细胞肿胀和出现大小不等的空泡,少量细胞脱落、坏死;BHD组有少量的肾小管上皮细胞脱落、坏死。Masson染色结果显示,与对照组比较,CIH组肾小球周围出现明显的纤维化, BHD组纤维化明显减少。Western blot显示,与对照组比较,CIH组肾组织NLRP3、caspase-1、IL-1β及IL-18的蛋白水平显著升高(P<0.01,P<0.05),与CIH组比较,BHD组显著下降(P<0.01,P<0.05)。免疫组织化学染色显示,与对照组比较,CIH组NLRP3、caspase-1和IL-1β的蛋白表达显著增多(P<0.01),且NLRP3在肾小管上皮细胞和间质巨噬细胞内表达最多,caspase-1和IL-1β主要见于肾小管上皮细胞胞浆,经BHD治疗后,与CIH组比较,BHD组NLRP3、caspase-1和IL-1β表达显著下降(P<0.05)。结论: 半夏厚朴汤可通过抑制NLRP3/Casapse-1/IL-1β信号通路相关分子的表达,减轻肾脏炎性损伤。

关键词: 半夏厚朴汤, 慢性间歇性低氧, 肾脏, NLRP3

Abstract: Objective: To investigate the effects of Banxia Houpo decoction on the renal NLRP3/Caspase-1/IL-1β signaling pathway in chronic intermittent hypoxia mice. Methods: C57BL/6 mice were randomly divided into 3 groups, normal control group (Control), chronic intermittent hypoxia group (CIH), and Banxia Houpo decoction treatment group (BHD), with 10 mice in each group. Mice in the CIH group and BHD group were placed in a hypoxic chamber. The oxygen volume fraction in the cabin was decreased from 21% to 9% in 90 s, and then oxygen was filled in 90 s to gradually increase the oxygen volume fraction in the cabin to 21%, while the mice in the control group were placed in the cabin and filled with normal air, processing 8 hours per day for 21 days. The mice in BHD group were treated with Banxia Houpu decoction by gavage before entering the cabin every day, and the control group and CIH group were given an equal volume of normal saline. After modeling, the changes of renal function indexes in each group were detected; HE and Masson staining were used to observe the pathological conditions of the kidney; Western blot and immunohistochemical staining were used to detect the protein expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3), aspartate-specific cysteine protein 1(Caspase-1) and interleukine-1beta(IL-1β). Results: Compared with control group, the contents of serum renal functional indexes UA, BUN and SCr in CIH group were increased significantly (P<0.01), and after BHD treatment, they all were decreased significantly compared with CIH group (P<0.01). Compared with control group, the results of HE staining showed that in the CIH group, glomerular endothelial cells were degenerated and necrotic, and vacuoles of different sizes appeared in renal tubular epithelial cells, and a small amount of renal tubular epithelial cells fell off and died. The pathological condition of the BHD group was improved compared with CIH group, the glomerular morphology gradually returned to normal, and a small amount of renal tubular epithelial cells fell off and died. Compared with control group, Masson staining results showed that there was obvious fibrosis around the glomeruli in the CIH group, the fibrosis was significantly reduced in the BHD group. The expression levels of NLRP3, Caspase-1, IL-1β and IL-18 were increased significantly compared with control group (P<0.05 or P<0.01), and immunohistochemical staining showed that NLRP3 was mainly expressed in renal tubular epithelial cells and interstitial macrophages, caspase-1 and IL-1β were mainly found in the cytoplasm of renal tubular epithelial cells. After BHD treatment, the expression levels of each protein were decreased compared with CIH group (P<0.05). Conclusion: Banxia Houpu decoction can reduce the kidney damage by inhibiting the expression of related molecules in the NLRP3/Casapse-1/IL-1β signaling pathway.

Key words: Banxia Houpo decoction, chronic intermittent hypoxia, kidney, NLRP3

中图分类号:

R363.2

刘寒, 郭亚净, 赵亚硕, 李亭亭, 吉恩生. NLRP3/Caspase-1通路对半夏厚朴汤干预慢性间歇性低氧小鼠肾脏炎性损伤中的作用^*[J]. 中国应用生理学杂志, 2022, 38(4): 295-299.

LIU Han, GUO Ya-jing, ZHAO Ya-shuo, LI Ting-ting, JI En-sheng. Effects of NLRP3/Caspase-1 pathway on Banxia Houpu decoction in the intervention of chronic intermittent hypoxia in mice with renal inflammatory injury[J]. CJAP, 2022, 38(4): 295-299.

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NLRP3/Caspase-1通路对半夏厚朴汤干预慢性间歇性低氧小鼠肾脏炎性损伤中的作用^*

Effects of NLRP3/Caspase-1 pathway on Banxia Houpu decoction in the intervention of chronic intermittent hypoxia in mice with renal inflammatory injury

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