维生素C对镉负荷小鼠睾丸抗氧化功能的影响*

doi:10.12047/j.cjap.6258.2022.043

中国应用生理学杂志 ›› 2022, Vol. 38 ›› Issue (3): 233-237.doi: 10.12047/j.cjap.6258.2022.043

维生素C对镉负荷小鼠睾丸抗氧化功能的影响^*

周健¹, 秦浩¹, 李晓红^1△, 王永辉^2△

1.潍坊医学院公共卫生学院, 山东潍坊 261053;
2.军事科学院军事医学研究院环境医学与作业医学研究所, 天津 300050

收稿日期:2021-12-24 修回日期:2022-04-29 出版日期:2022-05-28 发布日期:2022-09-05
通讯作者: ^△Tel: 0536-8462432, 13212119945; E-mail: Lixh118@163.com, wyhui77@sohu.com

Effects of vitamin C on antioxidant function of testis in cadmium-loaded mice

ZHOU Jian¹, QIN Hao¹, LI Xiao-hong^1△, WANG Yong-hui^2△

1. School of Public Health, Weifang Medical College, Weifang 261053;
2. Institute of Environmental and Operational Medicine, Academy of Military Medical Sciences, Academy of Military Science, Tianjin 300050, China

Received:2021-12-24 Revised:2022-04-29 Online:2022-05-28 Published:2022-09-05

摘要/Abstract

摘要： 目的: 分析镉(Cd)负荷不同时间对小鼠睾丸抗氧化酶的影响及维生素C(VC)的保护作用。方法: 清洁级雄性昆明小鼠72只分为4组(n=18):对照组、Cd组(CdCl₂3 mg/kg)、VC组(200 mg/kg)、VC(200 mg/kg)+ Cd(CdCl₂3 mg/kg)组,每日染毒1次,染毒1 d和3 d及同时补充VC保护,第1日和第3日染毒24 h后,每组取半数小鼠称重,取血清和睾丸组织;检测睾丸脏器系数,血清和睾丸组织丙二醛(MDA)、超氧化物歧化酶(SOD),及睾丸组织谷胱甘肽过氧化物酶(GSH-Px)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)及总谷胱甘肽(T-GSH)。结果: 与对照组比较,Cd组1 d和3 d小鼠体重和睾丸脏器系数下降;染毒3 d,Cd组小鼠血清SOD显著降低、MDA显著升高(P＜0.05);Cd组1 d小鼠睾丸的SOD、GSH-Px、T-GSH及GSH/GSSG显著升高(P＜0.05),而3 d的上述指标均显著降低(P＜0.05),Cd组1 d和3 d MDA水平均显著升高(P＜0.05);VC处理后减轻的程度有所降低。与Cd组比较,VC+ Cd组血清SOD和MDA水平在染毒3 d变化有显著性差异(P＜0.05);VC+ Cd组在染毒1 d和3 d,小鼠睾丸的SOD、GSH-Px、T-GSH及GSH/GSSG水平变化有显著性差异(P＜0.05),VC+ Cd组在染毒3 d睾丸的MDA水平显著降低(P＜0.05)。与Cd组1 d比较,染毒3 d小鼠的血清SOD水平显著降低(P＜0.05),睾丸指标变化也有显著性差异(P＜0.05)。结论: VC处理可在一定程度上改善镉负荷小鼠的抗氧化功能,对睾丸氧化损伤具有保护作用。

关键词: 镉, 维生素C, 抗氧化酶, 睾丸, 小鼠

Abstract: Objective: To investigate the effects of cadmium (Cd) on antioxidant enzymes in testis of mice and the protective effect of vitamin C (VC). Methods: A total of 72 male Kunming mice of clean grade were divided into four groups (n=18): the control group, the Cd group (CdCl₂3 mg/kg), the VC group (200 mg/kg), and the VC (200 mg/kg) +Cd group (CdCl₂3 mg/kg). Mice were poisoned once a day, exposed for 1 and 3 days and were treated with VC at the same time. Twenty-four hours after exposure on the 1st and 3rd day, half of the mice in each group were weighed, the serum and testis tissues were collected. Testicular organ coefficient, malondialdehyde (MDA) and superoxide dismutase (SOD) in serum and testis tissues, and glutathione peroxidase (GSH-Px), reduced glutathione (GSH), oxidized glutathione (GSSG) and total glutathione (T-GSH) in testis tissues were detected. Results: Compared with the control group, the body weight and testicle organ coefficient of mice in the Cd group were decreased on the 1st and 3rd day; after 3 days of exposure, the serum SOD in the Cd group was decreased significantly and MDA was increased significantly (P＜0.05); the levels of SOD, GSH-Px, T-GSH and GSH/GSSG of testis in the Cd group were increased significantly on the 1st day (P＜0.05), while all the above indexes were decreased significantly on the 3rd day (P＜0.05), and the content of MDA was increased significantly on the 1st and 3rd days in the Cd group (P＜0.05); after VC treatment, the degree of reduction was decreased. Compared with the Cd group, the serum SOD and MDA levels in the VC+ Cd group were significantly different after 3 days of exposure (P＜0.05); the changes of SOD, GSH-Px, T-GSH and GSH/GSSG levels of the testis in the VC+ Cd group were significantly different on the 1st and 3rd day of exposure (P＜0.05), and the MDA level of the testis in the VC+ Cd group was decreased significantly on the 3rd day of exposure (P＜0.05). Compared with the Cd group for 1 day, the level of serum SOD exposed for 3 days was decreased significantly (P＜0.05), and the changes of testis indexes were also significantly different (P＜0.05). Conclusion: VC treatment can improve the antioxidant function of cadmium-loaded mice to some extent, and has protective effect on oxidative damage of testis.

Key words: cadmium, vitamin C, antioxidant enzymes, testicle, mice

中图分类号:

R15-1

周健, 秦浩, 李晓红, 王永辉. 维生素C对镉负荷小鼠睾丸抗氧化功能的影响^*[J]. 中国应用生理学杂志, 2022, 38(3): 233-237.

ZHOU Jian, QIN Hao, LI Xiao-hong, WANG Yong-hui. Effects of vitamin C on antioxidant function of testis in cadmium-loaded mice[J]. CJAP, 2022, 38(3): 233-237.

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维生素C对镉负荷小鼠睾丸抗氧化功能的影响^*

Effects of vitamin C on antioxidant function of testis in cadmium-loaded mice

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