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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (2): 112-115.doi: 10.13459/j.cnki.cjap.2016.02.005

• 研究论文 • 上一篇    下一篇

溃疡性结肠炎小鼠肠道通透性改变与TNF-α及NF-κB P65的关系

黄循铷1, 王承党1, 王瑞幸2, 焦海霞2   

  1. 1. 福建医科大学附属第一医院消化内科, 福建医科大学消化研究室, 福建医科大学第一临床学系, 福州 350004;
    2. 福建医科大学生理及病理生理学系, 福州 350004
  • 收稿日期:2015-07-28 修回日期:2016-01-28 出版日期:2016-03-28 发布日期:2018-06-12
  • 通讯作者: 焦海霞,Tel:13799369171;E-mail:haixiafjmu@126.com E-mail:haixiafjmu@126.com
  • 基金资助:
    福建省自然科学基金(2013J01127),福建省教育厅一般科技项目(JA12149)

Changes of colonic permeability and its correlation with TNF-α, NF-κB p65 in ulceration colitis mice

HUANG Xun-ru1, WANG Cheng-dang1, WANG Rui-xing2, JIAO Hai-xia2   

  1. 1. Department of Gastroenterology, the First Affiliate Hospital, Fujian Medical University, the Digestive Disease Lab of Fujian Medical University, Fujian 350005;
    2. Department of Physiology and Pathophysiology, Fujian Medical University, Fujian 350108, China
  • Received:2015-07-28 Revised:2016-01-28 Online:2016-03-28 Published:2018-06-12
  • Supported by:
    福建省自然科学基金(2013J01127),福建省教育厅一般科技项目(JA12149)

摘要: 目的:采用2.5%葡聚糖硫酸钠(DSS)定量灌胃诱导小鼠溃疡性结肠炎(UC),观察小鼠结肠通透性改变与肿瘤坏死因子α(TNF-α)及NF-κB p65的关系。方法:48只ICR小鼠随机分为2组(n=24):对照组和模型组。模型组小鼠给予2.5% DSS定量灌胃诱发小鼠急性UC,对照组小鼠予同体积的蒸馏水灌胃代替。记录两组小鼠疾病活动指数(DAI),9 d后测定两组小鼠结肠组织病理学评分、结肠通透性、TNF-α及NF-κB p65。统计分析DAI、结肠通透性、TNF-α与NF-κB p65之间的相关性。结果:与对照组比较,模型组小鼠DAI、结肠病理学评分、结肠通透性、TNF-α、NF-κB p65均显著增高(P均<0.01)。小鼠DAI增高与结肠通透性密切相关(P均<0.01),结肠通透性增高与TNF-α、NF-κB p65密切相关(P均<0.01)。结论:与对照组小鼠相比,DSS造模小鼠的结肠通透性显著增高,并与TNF-α、NF-κB p65增高呈正相关。TNF-α、NF-κB p65增高导致结肠通透性增高,进而导致炎症免疫反应过度增强,可能是UC发病的重要环节。

关键词: 葡聚糖硫酸钠, 溃疡性结肠炎, 通透性:NF-κB p65, TNF-α, 小鼠

Abstract: Objective: To study the changes of colonic permeability and its correlation with TNF-α, NF-κB p65 in indextran sulphate sodium (DSS) -induced ulcerative colitis(UC) of mice. Methods: Forty-eight ICR mice were randomly divided into the control group and the model group. The acute UC model was induced by quantified intragastric administration of 2.5% DSS in mice. The disease activity index(DAI), histopathology scores, colonic permeability, expression of TNF-α, NF-κB p65 in colonic tissue were determined. The change of colonic permeability and its correlation with DAI, TNF-α, NF-κB p65 were analyzed. Results: Compareded with the control group, DAI colonic permeability of colonic tissue,and the expression of TNF-α NF-κB p65 in the model group were increased significantly (P<0.01, P<0.01). The increased colonic permeability correlated with DAI (P<0.01), and the expression of TNF-α(P<0.01), NF-κB p65(P<0.01) changed significantly. Conclusion: The alteration of colonic permeability and increased expression of TNF-α, NF-κB p65 may play important roles in the occurrence and development of UC.

Key words: dextran sulfate sodium, ulcerative colitis, permeability, NF-κB p65, TNF-α, mice

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