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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (2): 137-141.doi: 10.13459/j.cnki.cjap.2016.02.011

• 研究论文 • 上一篇    下一篇

当归挥发油对哮喘BALB/c小鼠的平喘作用及对Th17免疫活性的影响

妥海燕1, 任远1,2, 王志旺1,2, 程小丽1, 刘雪枫1, 李荣科1, 王瑞琼1   

  1. 1. 甘肃中医药大学, 兰州 730000;
    2. 甘肃省中药药理与毒理学重点实验室, 兰州 730000
  • 收稿日期:2015-09-17 修回日期:2016-01-25 出版日期:2016-03-28 发布日期:2018-06-12
  • 通讯作者: 任远,Tel:0931-8765395;E-mail:13893109787@126.com;王志旺,Tel:0931-8765395;E-mail:wzw0933@126.com E-mail:13893109787@126.com;wzw0933@126.com
  • 基金资助:
    国家自然科学基金项目(81460668);甘肃省自然科学基金项目(1310RJZA086);甘肃中医药大学研究生创新基金项目(2015CX23)

Effects of VOR on asthmatic BALB/c mice and on immune-activity of Th17 cells

TUO Hai-yan1, REN Yuan1,2, WANG Zhi-wang1,2, CHENG Xiao-li1, LIU Xue-feng1, LI Rong-ke1, WANG Rui-qiong1   

  1. 1. Gansu University of Traditional Chinese Medicine, Lanzhou 730000;
    2. Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicine of Gansu Province, Lanzhou 730000, China
  • Received:2015-09-17 Revised:2016-01-25 Online:2016-03-28 Published:2018-06-12
  • Supported by:
    国家自然科学基金项目(81460668);甘肃省自然科学基金项目(1310RJZA086);甘肃中医药大学研究生创新基金项目(2015CX23)

摘要: 目的:探讨当归挥发油对哮喘BALB/c小鼠的平喘作用及对IL-17A、RORγt等Th17细胞活性因子的影响。方法:取雌性小鼠随机分为7组(n=12):正常对照组、模型对照组、地塞米松组、当归挥发油高中低剂量组及当归油中+地米组,通过注射卵清白蛋白(OVA)致敏、吸入OVA激发的方法来复制哮喘动物模型,在当归挥发油的防治下,考察当归挥发油对哮喘行为学、肺功能、肺组织病理学、血清IL-17A及肺组织RORγt表达的影响。结果:60、120、240 mg/kg当归挥发油可维持哮喘小鼠体重的正常增长,改善哮喘行为学、肺功能及肺组织病理学变化,抑制IL-17A与RORγt的过度表达(P<0.05, 0.01)。同时,当归挥发油与地塞米松配伍后对维持体重的正常增长、缓解IL-17A与RORγt的过度表达具有一定的协同作用。结论:当归挥发油具有明显的防治哮喘作用,抑制IL-17、RORγt过度表达而抑制Th17细胞免疫活性是其作用机制之一;而当归挥发油与糖皮质激素配伍后有一定的协同作用。

关键词: 当归挥发油, BALB/c小鼠, 平喘, Th17细胞

Abstract: Objective: To study the anti-asthmatic effects of Volatile oil of Radix Angelicae Sinensis (VOR, Traditional Chinese Medicine) on asthmatic BALB/c mice and its effect on Th17 cell immuno-activity through IL-17A and RORγt. Methods: After grouping (n=12), the asthmatic BALB/c mice were replicated through injection of ovalbumin (OVA) for sensitization and administration of OVA aerosol for challenge and then, the asthmatic behaviors, respiratory function, lung histopathology as well as levels of IL-17A in serum and retinoid-related orphan receptor gamma t (RORγt) in lung tissue were observed after the action of VOR. Results: VOR could maintain normal growth of body weight in asthmatic mice, improve asthmatic behaviors, respiratory function, lung histopathology and, inhibit over-expression of IL-17A and RORγt (P<0.05, 0.01) at 60, 120, 240 mg/kg doses. The combination of VOR and dexamethasone could bring synergistic effects on growth of body weight and expression of IL-17A and RORγt. Conclusion: VOR has significant effects of anti-asthma and one of the mechanisms is to inhibit immune activity of Th17 cell through relieving over-expression of IL-17 and RORγt. Besides, the combination of VOR and glucocorticoid could bring synergistic effects.

Key words: Volatile oil of Radix Angelicae Sinensis (VOR), BALB/c mice, antiasthma, Th17 cell

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