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中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (3): 270-273.doi: 10.13459/j.cnki.cjap.2016.03.021

• 研究论文 • 上一篇    下一篇

胍丁胺对大鼠应激性体温升高的抑制作用

熊资, 杨永录, 胥建辉, 卜舒, 罗蓉, 陈冰   

  1. 成都医学院体温与炎症 四川省高校重点实验室, 成都 610500
  • 收稿日期:2015-12-07 修回日期:2016-02-14 出版日期:2016-05-28 发布日期:2018-06-12
  • 通讯作者: 杨永录,Tel:028-62739330;E-mail:ylyang9@sohu.com E-mail:ylyang9@sohu.com
  • 基金资助:
    四川省教育厅科研基金资助项目(12ZB204,15ZA0251)

Inhibitory effects of agmatine on stress-induced hyperthermia in rats

XIONG Zi, YANG Yong-lu, XU Jian-hui, BU Shu, LUO Rong, CHEN Bing   

  1. Key Laboratory of Thermoregulation and Inflammation of Sichuan Higher Education Institutes, Chengdu Medical College, Chengdu 610500, China
  • Received:2015-12-07 Revised:2016-02-14 Online:2016-05-28 Published:2018-06-12
  • Supported by:
    四川省教育厅科研基金资助项目(12ZB204,15ZA0251)

摘要: 目的:观察胍丁胺(AGM)是否能降低或反转应激性体温过高反应。方法:61只雄性SD大鼠随机分为3部分,每部分再分为对照组和AGM组。在实验过程中,人工气候箱和开放实验箱内的温度均保持在22℃。①用无线遥测技术连续测量大鼠的体温和活动,观察腹腔注射AGM对安静状态下大鼠正常体温和活动的影响(n=8);②将大鼠放置在开放实验箱中60 min复制应激性体温过高的模型,用无线遥测技术连续测量开放实验箱内大鼠体温和活动的变化(n=7~8);③用美国哥伦布公司动物代谢分析系统测量AGM对大鼠能量代谢的影响(n=7)。结果:①腹腔注射AGM 80 mg/kg能引起正常大鼠出现明显低温反应(-0.46±0.11)℃,而注射AGM 40 mg/kg则对正常体温无明显影响。②对照组大鼠腹腔注射生理盐水后,置于开放实验箱内体温升高达(0.78±0.16)℃;给大鼠注射AGM 40或80 mg/kg后,置于开放实验箱内60 min时,体温则分别降低(0.34±0.11)℃和(0.81±0.14)℃。③AGM 80 mg/kg能明显降低大鼠的耗氧量和产热量。结论:AGM能引起正常大鼠出现低温反应和明显翻转应激性体温升高反应,其作用可能与AGM能降低能量代谢有关。

关键词: 胍丁胺, 应激性体温过高, 低温反应, 能量代谢, 大鼠

Abstract: Objective:To examine whether agmatine (AGM) would alter stress-induces hyperthermic response. Methods:Sixty-one male SD rats were randomly divided into three experiments. Each experiment was divided into control group and AGM group. During the experiments, the animals were maintained in a chamber at 22℃. ①Effects of intraperitoneal injecting 40 or 80 mg/kg AGM on normal core temperature and activity were observed in undisturbed rats using radiotelemetry (n=8). ②Stress-induced hyperthermia model was established by placing rats in an open-field chamber for 60 min. Rats were dosed intraperitoneally with AGM or saline, and placed immediately inside the open-field chamber. Core temperature and motor activity were monitored by radiotelemetry in an open-field chamber (n=7~8). ③Effect of AGM on energy metabolism was measured by Columbus Oxymax Lab Animal Monitoring System (n=7). Results:①Rats administered with 80 mg/kg AMG showed significant hypothermic responses (-0.46±0.11)℃, while 40 mg/kg AMG had no significant effect on the normal core temperature. ②Core temperature of control group increased by (0.78±0.16)℃ during open-field exposure, whereas rats administered 40 and 80 mg/kg AGM underwent a (0.34±0.11)℃ and (0.81±0.14)℃ reductions in core temperature within 60 min, respectively. ③Oxygen consumption and energy metabolism were significantly reduced by AGM (80 mg/kg). Conclusion:The data demonstrated that AGM induced hypothermic responses in rats and reversed stress-induced hyperthermia, and its effect might attribute to the suppression of energy metabolism.

Key words: agmatine, stress hyperthermia, hypothermic response, energy metabolism, rats

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