川芎嗪对模拟失重大鼠颈动脉平滑肌Ca2+敏感性的影响

doi:10.13459/j.cnki.cjap.2016.04.019

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (4): 361-364.doi: 10.13459/j.cnki.cjap.2016.04.019

川芎嗪对模拟失重大鼠颈动脉平滑肌Ca²⁺敏感性的影响

崔艳领, 刘甜, 孙晗, 张月, 冯甜, 王慧平

西北大学西部资源生物与现代生物技术教育部重点实验室, 陕西西安 710069

收稿日期:2015-04-18 修回日期:2016-02-23 出版日期:2016-07-28 发布日期:2018-06-20
通讯作者: 王慧平,Tel:029-88303935;E-mail:wanghp@nwu.edu.cn E-mail:wanghp@nwu.edu.cn
基金资助:
陕西省自然科学基金项目（2013JM3001）；陕西省教育厅自然科学专项项目（14JK1755）；西北大学校级大学生创新实验项目（2014004）

Effects of ligustrazine on the Ca²⁺ sensitivity in carotid artery smooth muscle of simulated weightlessness rats

CUI Yan-ling, LIU Tian, SUN Han, ZHANG Yue, FENG Tian, WANG Hui-ping

Key Laboratory of Resource Biology and Biotechnology in Western China, Northwest University, Ministry of Education, Xi'an 710069, China

Received:2015-04-18 Revised:2016-02-23 Online:2016-07-28 Published:2018-06-20
Supported by:
陕西省自然科学基金项目（2013JM3001）；陕西省教育厅自然科学专项项目（14JK1755）；西北大学校级大学生创新实验项目（2014004）

摘要/Abstract

摘要： 目的：探讨川芎嗪对模拟失重大鼠颈动脉平滑肌收缩蛋白Ca²⁺敏感性的影响及机制。方法：21只雌性SD大鼠分为对照组（CON）、模拟失重组（TS）、模拟失重+川芎嗪灌饲组（LTZ）（n=7）。4周后测定大鼠颈动脉收缩性、收缩蛋白Ca²⁺敏感性及肌球蛋白轻链激酶抑制剂ML-7对以上指标的影响。结果：TS组苯肾上腺素（PHE）收缩力比CON组高25.14%（P<0.01），LTZ组较TS组低13.46%（P<0.01），较CON组高8.30%；ML-7可使CON、TS、LTZ组PHE收缩力分别降低8.84%、16.24%（P<0.01）和3.40%；TS组KCl收缩力比CON组高40.46%（P<0.01），LTZ组较TS组低18.80%，较CON组高14.05%；ML-7可使CON、TS、LTZ组KCl收缩力分别降低21.97%（P<0.05）、21.88%（P<0.01）和10.84%；TS组pD2[Ca²⁺]比CON组高10.03%（P<0.01），LTZ组较TS组低7.01%（P<0.01），较CON组高2.32%；ML-7可使CON、TS、LTZ组pD2[Ca²⁺]分别降低2.42%、7.43%（P<0.01）和2.51%。结论：模拟失重大鼠颈动脉收缩增强可能是由动脉平滑肌收缩蛋白Ca²⁺敏感性增强所导致的。川芎嗪可改善模拟失重大鼠颈动脉的收缩功能，其机制可能与抑制血管平滑肌肌球蛋白轻链激酶继而降低Ca²⁺敏感性有关。

关键词: 模拟失重, 颈动脉, 川芎嗪, Ca²⁺敏感性, 肌球蛋白轻链激酶, 大鼠

Abstract: Objective:To study the effects of Ligustrazine on the Ca²⁺ sensitivity in carotid artery smooth muscle of simulated weightless-ness rats. Methods:Twenty-one female SD rats were divided into control group (CON), simulated weightlessness group (TS), simulated weightlessness plus Ligustrazine administration group (LTZ) (n=7). After 4 weeks, the contractile property and the Ca²⁺ sensitivity in carotid artery were measured. Furthermore, the effects of ML-7(a myosin light chain kinase specific inhibitor) on above mentioned parameters were recorded. Results:Compared with CON group, the contractile force induced by phenylephrine (PHE) in TS group was increased by 25.14% (P<0.01). The force in LTZ group decreased by 13.46% (P<0.01) and increased by 8.30% compared with TS and CON group, respec-tively. After the artery rings were incubated with ML-7, the force in CON, TS and LTZ group were reduced by 8.84%, 16.24% (P<0.01) and 3.40%, respectively. Compared with CON group, the contractile force induced by KCl in TS group was increased by 40.46% (P<0.01). The force in LTZ group decreased by 18.80% and increased by 14.05% compared with TS and CON group, respectively. The force in CON, TS and LTZ group were reduced by 21.97% (P<0.05), 21.88% (P<0.01) and 10.84% by ML-7, respectively. Compared with CON group, the pD2[Ca²⁺] in TS group was increased by 10.03% (P<0.01). The pD2[Ca²⁺] in LTZ group was decreased by 7.01% (P<0.01) and increased by 2.32% compared with TS and CON group, respectively. The pD2[Ca²⁺] in CON, TS and LTZ group was reduced by 2.42%, 7.43% (P<0.01) and 2.51% by ML-7, respectively. Conclusion:The contraction of carotid artery is strength-ened in simulated weightlessness rats, it maybe results from the Ca²⁺ sensitivity of contractile proteins in smooth muscle increasing. Ligus-trazine can improve the contraction of carotid artery, reducing the Ca²⁺ sensitivity and inhibiting myosin light chain kinase may be involved in its mechanisms.

Key words: simulated weightlessness, carotid artery, Ligustrazine, Ca²⁺ sensitivity, myosin light chain kinase, rats

崔艳领, 刘甜, 孙晗, 张月, 冯甜, 王慧平. 川芎嗪对模拟失重大鼠颈动脉平滑肌Ca²⁺敏感性的影响[J]. 中国应用生理学杂志, 2016, 32(4): 361-364.

CUI Yan-ling, LIU Tian, SUN Han, ZHANG Yue, FENG Tian, WANG Hui-ping. Effects of ligustrazine on the Ca²⁺ sensitivity in carotid artery smooth muscle of simulated weightlessness rats[J]. CJAP, 2016, 32(4): 361-364.

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川芎嗪对模拟失重大鼠颈动脉平滑肌Ca²⁺敏感性的影响

Effects of ligustrazine on the Ca²⁺ sensitivity in carotid artery smooth muscle of simulated weightlessness rats

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