延龄草对LPS诱导大鼠肝损伤的保护作用及机制

doi:10.13459/j.cnki.cjap.2016.04.022

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (4): 373-377.doi: 10.13459/j.cnki.cjap.2016.04.022

延龄草对LPS诱导大鼠肝损伤的保护作用及机制

詹光杰^1,2, 肖本见², 杨年安³

1. 生物资源保护与利用湖北省重点实验室, 恩施 445000;
2. 湖北民族学院科技学院, 恩施 445000;
3. 风湿疾病发生与干预湖北省重点实验室, 恩施 445000

收稿日期:2015-06-05 修回日期:2016-01-29 出版日期:2016-07-28 发布日期:2018-06-20
通讯作者: 詹光杰,Tel:13135832144;E-mail:zhan_323@163.com E-mail:zhan_323@163.com
基金资助:
生物资源保护与利用湖北省重点实验室资助（PKL-HB1514）；林学一级学科资助

The protective effects and mechanisms of Trillium tschonoskii Maxim on rats' liver damage induced by lipopolysaccharide

ZHAN Guang-jie^1,2, XIAO Ben-jian², YANG Nian-an³

1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, EnShi 445000, China;
2. Science and Technology College of Hubei University for Nationalities, EnShi 445000, China;
3. Hubei Provincial Key Laboratory of Occurrence and Intervention of Rhumatic Diseases, EnShi 445000, China

Received:2015-06-05 Revised:2016-01-29 Online:2016-07-28 Published:2018-06-20
Supported by:
生物资源保护与利用湖北省重点实验室资助（PKL-HB1514）；林学一级学科资助

摘要/Abstract

摘要： 目的：研究延龄草（TTM）对脂多糖（LPS）诱导大鼠氧化应激与肝损伤的保护作用。方法：SD大鼠60只，按体重随机分成TTM高、中、低剂量组、模型组、地塞米松磷酸钠（DEX）对照组及空白对照组（n=10）。TTM高、中及低剂量组按（8、4、2） g/（kg·d） TTM灌胃，模型组、DEX对照组及空白对照组灌胃等量蒸馏水，每隔5 d，TTM高、中、低剂量组、模型组、DEX对照组按1 mg/kg腹腔注射LPS，DEX对照组同时腹腔注射DEX（5 mg/kg），空白对照组注射等量生理盐水。30 d后，测定大鼠胸腺指数、脾脏指数，对血清一氧化氮合酶（NOS）、超氧化物歧化酶（SOD）活性与一氧化氮（NO）、谷胱甘肽（GSH）、硫代巴比妥酸反应产物（TBARS）、白细胞介素6（IL-6）、IL-10及肿瘤坏死因子α（TNF-α）含量，肝组织SOD、谷胱甘肽过氧化氢酶（GSH-Px）活性与GSH、TBARS含量进行检测。结果：与模型组相比，TTM高剂量组在（19~30） d体重显著降低（P<0.05），TTM高、中、低剂量组胸腺指数，TTM高剂量组脾脏指数显著降低（P<0.05），TTM高、中、低剂量组血清NOS活性与TBARS、NO含量显著降低（P<0.05），TTM高剂量组血清SOD活性及中、高剂量组GSH含量显著上升（P<0.05），TTM高、中剂量组血清IL-6、TNF-α含量显著降低，IL-10含量显著升高（P<0.05），TTM中、高剂量组肝脏TBARS含量显著降低，TTM各剂量组肝脏SOD活性与中、高剂量组GSH-Px活性，高剂量组GSH含量显著升高（P<0.05）。结论：TTM对LPS所致大鼠的胸腺、脾脏萎缩有一定的延缓作用，能有效降低血清中NOS活性，减少NO生成，提升SOD、GSH-Px活性与GSH含量，减轻脂质过氧化，降低IL-6、TNF-α过量分泌、提升IL-10含量，有抗炎护肝的功能。

关键词: 延龄草, 脂多糖, 肝损伤, 抗氧化, 抗炎

Abstract: Objective:To study the protective effects of Trillium tschonoskii maxim (TTM) on rats' oxidative stress and hepatotoxicity in-duced by lipopolysaccharide (LPS). Methods:Sixty SD rats were randomly divided into TTM high, medium and low dose groups, model group, Dexamethasone (DEX) control group and blank control group with ten rats in each group. The TTM high, medium and low dose groups were treated with 8, 4, 2 g/(kg·d) TTM by intragastric administration and model group, DEX control group and blank control group were treated with the same amount of distilled water respectively. The TTM high, medium and low dose groups, model group, DEX control group were injected intraperitoneal with 1 mg/kg LPS and the DEX control group was injected intraperitoneal with 5 mg/kg DEX, the blank control group was injected with same amount of normal saline every five days. The indexes of rats' thymus and spleen were measured in 30 days. The activities of serum nitric oxide synthase (NOS), superoxide dismutase (SOD) and the contents of nitric oxide (NO), glutathione (GSH), glu-cosinolates barbituric acid reaction product(TBARS), white cells interleukin-6(IL-6), IL-10 and tumor necrosis factor-α(TNF-α), the activi-ties of liver SOD, GSH-Px and the contents of GSH and TBARS were measured. Results:TTM high dose group was significantly different in body weight in 19~30 days(P<0.05); The index of thymus in TTM high, medium and low dose groups and the index of spleen in TTM high dose group were decreased significantly compared with those of the model group. The activity of serum NOS and the contents of TBARS and NO in TTM high, medium and low dose groups were decreased significantly(P<0.05). The activity of serum SOD in TTM high dose group and the contents of GSH in TTM medium and low dose groups were increased significantly(P<0.05). The contents of serum IL-6 and TNF-αin TTM high, medium dose groups were decreased significantly and the contents of serum IL-10 were increased significantly(P<0.05). The con-tents of liver TBARS in TTM high, medium dose groups were decreased significantly. The activity of liver SOD in TTM high, medium and low dose groups, the activity of GSH-Px in TTM high, medium dose groups and the contents of GSH in TTM high dose group were increased signifi-cantly(P<0.05). Conclusion:TTM has a certain effect to delay the rats' atrophy of thymus and spleen generated by LPS. It can effectively reduce the activity of NOS in serum, reduce the formation of NO, improve the activity of SOD, GSH-Px and the contents of GSH, reduce lipid peroxidation, decrease the excessive secretion of IL-6, TNF-α, increase the contents of IL-10, which can resist inflammation and protect the liver.

Key words: Trillium tschonoskii Maxim(traditional Chinese medicine), lipopolysaccharide, liver damage, antioxidant, anti-in-flammatory

詹光杰, 肖本见, 杨年安. 延龄草对LPS诱导大鼠肝损伤的保护作用及机制[J]. 中国应用生理学杂志, 2016, 32(4): 373-377.

ZHAN Guang-jie, XIAO Ben-jian, YANG Nian-an. The protective effects and mechanisms of Trillium tschonoskii Maxim on rats' liver damage induced by lipopolysaccharide[J]. CJAP, 2016, 32(4): 373-377.

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延龄草对LPS诱导大鼠肝损伤的保护作用及机制

The protective effects and mechanisms of Trillium tschonoskii Maxim on rats' liver damage induced by lipopolysaccharide

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