低氧性肺动脉高压小鼠体内脂质水平的变化

doi:10.13459/j.cnki.cjap.2016.05.020

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (5): 463-465.doi: 10.13459/j.cnki.cjap.2016.05.020

低氧性肺动脉高压小鼠体内脂质水平的变化

范风云, 沈婉婷, 郑青青, 高媛, 胡凯媛, 范小芳, 毛孙忠, 龚永生

温州医科大学低氧医学研究所, 浙江温州 325035

收稿日期:2015-06-23 修回日期:2016-05-12 出版日期:2016-09-28 发布日期:2018-06-20
通讯作者: 龚永生,Tel:0577-86699521;E-mail:fxbwzmc@126.com E-mail:fxbwzmc@126.com
基金资助:
浙江省自然基金（LY14H010005）；浙江省大学生科技创新项目（2014R413029）

Changes of lipid levels in mice with hypoxic pulmonary arterial hypertension

FAN Feng-yun, SHEN Wan-ting, ZHENG Qing-qing, GAO Yuan, HU Kai-yuan, FAN Xiao-fang, MAO Sun-zhong, GONG Yong-sheng

Institute of Hypoxia Medicine, Wenzhou Medical University, Wenzhou 325035, China

Received:2015-06-23 Revised:2016-05-12 Online:2016-09-28 Published:2018-06-20
Supported by:
浙江省自然基金（LY14H010005）；浙江省大学生科技创新项目（2014R413029）

摘要/Abstract

摘要： 目的：观察低氧性肺动脉高压小鼠体内脂质代谢的变化，探讨脂质代谢异常在低氧性肺动脉高压发生发展中的意义。方法：SPF级雄性C57BL/6小鼠20只，随机分为2组（n=10）：常氧组和低氧组。常压连续低氧3周（9%~11% O₂，23 h/d）复制慢性低氧性肺动脉高压模型，测定小鼠右心室压（RVSP）和右心室与左心室加室间隔重量比，Elisa法检测血浆中总胆固醇（TC）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）的含量；real-time PCR法检测肝组织中3-羟基-3-甲基戊二酸单酰辅酶A还原酶（HMGCR）、低密度脂蛋白受体（LDLR）、清道夫受体B1（SR-B1）、固醇调节元件结合因子2（SREBF2）等基因的表达。结果：低氧组小鼠RVSP、RV/（LV+S）显著高于常氧组（P<0.05），血浆中HDL含量及HDL/LDL比值较常氧组显著降低（P<0.05），肝组织中LDLR、SR-B1基因表达较常氧组显著下调（P<0.05）；RVSP与HDL/LDL比值及LDLR、SR-B1基因表达呈显著负相关（P<0.05）。结论：脂质代谢异常参与小鼠低氧性肺动脉高压的形成。

关键词: 脂质代谢, 肺动脉高压, 低氧, 小鼠

Abstract: Objective: To observe the changes of lipid levels in mice with pulmonary hypertension induced by hypoxia. Methods: The animal model of hypoxic pulmonary hypertension was established by exposing the mice to isobaric hypoxic chamberfor 3 weeks (23 h/d, regular chow feed). Twenty male C57BL/6 mice were randomlydivided into normoxia group and hypoxia group (n=10). The concentrations of total cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) in plasma were detected by Elisa method.The mRNA levels of HMG-CoAreductase (HMGCR), low density lipoprotein receptor (LDLR), scavenger receptor class B1 (SR-B1), and sterol regulatory element-binding factor-2 (SREBF2) in liverwere measured by real-time PCR. Results: ① The right ventricular systolic pressure (RVSP) and the weight ratio of right ventricle (RV) to left ventricle plus septum (LV+S) of hypoxia group were significantly higher than those of normoxia group (P<0.05).② The concentrations of HDL and HDL/LDL in plasma were significantly higher in hypoxia group, compared with normoxia group (P<0.05).③The mRNA levels of LDLR and SR-B1in liver were significantly down-regulated in hypoxia group(P<0.05).④RVSP were significantly negative correlated with HDL/LDL, the gene expression of LDLR and SR-B1 (P<0.05). Conclusion: Abnormal lipid metabolism participates in the pathological proceeding of pulmonary hypertension induced by hypoxia.

Key words: lipid metabolism, pulmonary hypertension, hypoxia, mice

范风云, 沈婉婷, 郑青青, 高媛, 胡凯媛, 范小芳, 毛孙忠, 龚永生. 低氧性肺动脉高压小鼠体内脂质水平的变化[J]. 中国应用生理学杂志, 2016, 32(5): 463-465.

FAN Feng-yun, SHEN Wan-ting, ZHENG Qing-qing, GAO Yuan, HU Kai-yuan, FAN Xiao-fang, MAO Sun-zhong, GONG Yong-sheng. Changes of lipid levels in mice with hypoxic pulmonary arterial hypertension[J]. CJAP, 2016, 32(5): 463-465.

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低氧性肺动脉高压小鼠体内脂质水平的变化

Changes of lipid levels in mice with hypoxic pulmonary arterial hypertension

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