亚低温对脊髓损伤后反应性星形胶质细胞增生的影响

doi:10.13459/j.cnki.cjap.2016.04.001

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (4): 289-292.doi: 10.13459/j.cnki.cjap.2016.04.001

• 研究论文 • 下一篇

亚低温对脊髓损伤后反应性星形胶质细胞增生的影响

康文博^1,2, 李晓红¹, 陈羽中¹, 王景景¹, 涂悦¹, 张赛^1,2, 梁海乾¹

1. 武警后勤学院附属医院脑科医院, 脑创伤与神经疾病研究所, 天津市神经创伤修复重点实验室, 天津 300162;
2. 河北医科大学, 石家庄 050017

收稿日期:2015-04-07 修回日期:2015-10-13 出版日期:2016-07-28 发布日期:2018-06-20
通讯作者: 梁海乾,Tel:022-60577125;E-mail:lianghaiqian@163.com E-mail:lianghaiqian@163.com
基金资助:
国家自然科学基金项目（81301050，81271392，81401067）；天津市应用基础与前沿技术研究计划（14JCQNJC10200，15JCYBJC28100）；中国博士后基金项目（2013M542583）

The effect of inhibition of hyperplasia on spinal cord reactive astrocytes by mild hypothermia

KANG Wen-bo^1,2, LI Xiao-hong¹, CHEN Chong¹, WANG Jing-jing¹, TU Yue¹, ZHANG Sai^1,2, LIANG Hai-qian¹

1. Institute of Traumatic Brain Injury and Neurology, Brain Hospital of Affiliated Hospital of Logistics College of Chinese People's Armed Police Forces, Tianjin Key Labrotary of Neurotrauma Repair, Tianjin 300162;
2. Hebei Medical University, Shijiazhuang 050017, China

Received:2015-04-07 Revised:2015-10-13 Online:2016-07-28 Published:2018-06-20
Supported by:
国家自然科学基金项目（81301050，81271392，81401067）；天津市应用基础与前沿技术研究计划（14JCQNJC10200，15JCYBJC28100）；中国博士后基金项目（2013M542583）

摘要/Abstract

摘要： 目的：观察在不同温度条件下脊髓星形胶质细胞划痕损伤活化后的形态和活性改变，以探讨亚低温对脊髓损伤后反应性星形胶质细胞增生的影响。方法：体外原代培养新生SD大鼠脊髓星形胶质细胞，以划痕实验制备反应性星形胶质细胞。亚低温选择33℃，细胞培养48 h。实验分为对照组、划痕组、亚低温组和划痕+亚低温组。各组在相应的时间点观察细胞形态，采用免疫荧光染色方法检测Nestin阳性率，MTT比色法观察细胞活性，PI染色方法观察细胞凋亡程度。结果：与对照组和亚低温组相比，划痕组和划痕+亚低温组细胞胞体肥大，周围突起增多、延展以及胞浆丰富，细胞生长率明显升高。与划痕组相比，划痕+亚低温组细胞变化减慢，周围突起减少，细胞长入划痕处所需时间增加，细胞Nestin阳性率、PI阳性率和细胞生长率明显降低，各结果差异显著（P<0.01）。结论：划痕损伤后星形胶质细胞活化为反应性星形胶质细胞并会增生，亚低温明显抑制脊髓反应性星形胶质细胞的活化增生，并可以抑制星形胶质细胞的凋亡。

关键词: 亚低温, 反应性星形胶质细胞, 脊髓损伤, 大鼠

Abstract: Objective:To observe changes in the morphology and activity of astrocytes in spinal cord at different temperatures after scratches treatment, and to investigate the effect of mild hypothermia on hyperplasia of reactive astrocytes. Methods:Spinal cord astrocytes were cultured from the neonatal SD rat, and reactive astrocytes were prepared by scratches treatment. Mild hypothermia choose 33℃, cell cul-ture 48 h. Cells were divided into control group、scratches group、mild hypothermia group and scratch+mild hypothermia group. Cell mor-phology of each group were observed in 0 h、6 h、12 h、24 h、48 h、3 d、5 d、7 d. Nestin positive rate were detected by using immunofluorescence staining method. Cell activity were observed by methyl thiazolyl tetrazolium salt (MTT) assay. The degree of apoptosis was observed by using PI staining method. Results:Compared with control group and mild hypothermia group, cell body in both scratches group and scratch+mild hypothermia group was hypertrophy, the protrusion around was increased and extend, the cytoplasm was enrich, and the growth rate was signif-icantly increased. Compared with scratches group, scratch+mild hypothermia group cells grew slowly, the protrusion around was decreased, and cells of the scratched area ingrowth significantly slowly. Nestin and PI positive rate of cells were also significantly lower. All the results had statistically significant differences (P<0.01). Conclusion:After scratch injury, astrocytes were activated to be reactive astrocytes and hyper-plasia. Mild hypothermia significantly inhibited spinal cord reactive astrocytes overgrowth and restrain astrocytes apoptosis.

Key words: mild hypothermia, spinal cord injury, rats

康文博, 李晓红, 陈羽中, 王景景, 涂悦, 张赛, 梁海乾. 亚低温对脊髓损伤后反应性星形胶质细胞增生的影响[J]. 中国应用生理学杂志, 2016, 32(4): 289-292.

KANG Wen-bo, LI Xiao-hong, CHEN Chong, WANG Jing-jing, TU Yue, ZHANG Sai, LIANG Hai-qian. The effect of inhibition of hyperplasia on spinal cord reactive astrocytes by mild hypothermia[J]. CJAP, 2016, 32(4): 289-292.

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亚低温对脊髓损伤后反应性星形胶质细胞增生的影响

The effect of inhibition of hyperplasia on spinal cord reactive astrocytes by mild hypothermia

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