产前应激对子代大鼠脑缺血/再灌注后星形胶质细胞的影响

doi:10.13459/j.cnki.cjap.2016.04.004

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (4): 301-304.doi: 10.13459/j.cnki.cjap.2016.04.004

产前应激对子代大鼠脑缺血/再灌注后星形胶质细胞的影响

王凌星, 黄红红, 陈雅芳, 蔡鸿潮, 钱家强

福建医科大学附属第二医院神经内科, 泉州 362000

收稿日期:2015-11-24 修回日期:2016-04-04 出版日期:2016-07-28 发布日期:2018-06-20
通讯作者: 黄红红,Tel:13600766682;E-mail:minghuaxin@139.com E-mail:minghuaxin@139.com
基金资助:
福建省自然科学基金项目（2015J01449）；福建省教育厅项目（JB13067）；泉州市技术研究与开发项目（2012Z35）；院苗圃基金（2012MP65）

The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats

WANG Ling-xing, HUANG Hong-hong, CHEN Ya-fang, CAI Hong-chao, QIAN Jia-qiang

Department of Neurology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China

Received:2015-11-24 Revised:2016-04-04 Online:2016-07-28 Published:2018-06-20
Supported by:
福建省自然科学基金项目（2015J01449）；福建省教育厅项目（JB13067）；泉州市技术研究与开发项目（2012Z35）；院苗圃基金（2012MP65）

摘要/Abstract

摘要： 目的：探讨产前应激对雄性子代大鼠大脑中动脉缺血/再灌注后星形胶质细胞的影响。方法：SD孕鼠随机分为有产前应激处理（妊娠第15到21天每日3次限制活动）和无产前应激处理，并对其雄性子代大鼠采用线栓法制备大脑中动脉闭塞（MCAO）模型，共分为产前应激+假手术组、MCAO模型组、产前应激+MCAO组（n=10），于再灌注后第5天检测脑梗死体积，免疫荧光双标染色检测缺血灶边缘区星形胶质细胞形态及促红细胞生成素肝细胞受体A4（EphA4）和胶质纤维酸性蛋白（GFAP）的共表达情况，并采用Western blot检测EphA4、GFAP和神经蛋白聚糖（Neurocan）蛋白表达。结果：产前应激+MCAO组子代大鼠脑梗死体积百分比、EphA4、GFAP和Neurocan蛋白表达均较MCAO组显著增加（P均<0.05），且GFAP阳性细胞形态学改变及EphA4/GFAP共表达也较MCAO组明显。结论：产前应激可能改变子代大鼠脑缺血/再灌注后星形胶质细胞上EphA4受体的表达，促进星形胶质细胞活化，产生神经蛋白聚糖。

关键词: 产前应激, 子代大鼠, 脑缺血, 星形胶质细胞

Abstract: Objective:To investigate the effects of prenatal stress on astrocytes after ischemia/reperfusion of cerebral middle artery in adult offspring rats. Methods:Pregnant rats were randomly assigned to prenatal stress treatment group, which was exposed to restraint three times daily in the last week of pregnancy, and no prenatal stress treatment group. Adult male offspring rats were subjected to transient focal cerebral ischemia by middle cerebral artery occlusion (MCAO). There were three groups:prenatal stress+sham group, MCAO group and prenatal stress+MCAO group (n=10). After 5 days of reperfusion, the infarct size was evaluated. The morphology of astrocytes, co-local-ization of erythropoietin-producing hepatocellular receptor A4 (EphA4) and glial fibrillary acidic protein (GFAP) were detected by double im-munofluorescent staining. And the protein expressions of EphA4, GFAP and Neurocan in peri-ischemic regions were detected by Western blot. Results:The infarct size and the expression of EphA4, GFAP and Neurocan were significantly increased in prenatal stress+MCAO group compared with MCAO group (all P<0.05). And the morphological changes of GFAP-positive astrocytes and co-localization of EphA4/GFAP were more obvious in prenatal stress+MCAO group compared with MCAO group. Conclusion:Prenatal stress may upregulate the expression of EphA4 on astrocytes in the offspring rats after cerebral ischemia/reperfusion, which promotes the reactivity of astrocyte and increases the ex-pression of neurocan.

Key words: prenatal stress, offspring rats, cerebral ischemia, astrocyte

王凌星, 黄红红, 陈雅芳, 蔡鸿潮, 钱家强. 产前应激对子代大鼠脑缺血/再灌注后星形胶质细胞的影响[J]. 中国应用生理学杂志, 2016, 32(4): 301-304.

WANG Ling-xing, HUANG Hong-hong, CHEN Ya-fang, CAI Hong-chao, QIAN Jia-qiang. The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats[J]. CJAP, 2016, 32(4): 301-304.

参考文献

[1] Rolls A, Shechter R, Schwartz M. The bright side of the glial scar in CNS repair[J]. Nat Rev Neurosci, 2009, 10(3):235-241.
[2] Teshigawara K, Kuboyama T, Shigyo M, et al. A novel compound, denosomin, ameliorates spinal cord injury via ax-onal growth associated with astrocyte-secreted vimentin[J]. Br J Pharmacol, 2013, 168(4):903-919.
[3] Shen LH, Li Y, Gao Q, et al. Down-regulation of neurocan expression in reactive astrocytes promotes axonal regeneration and facilitates the neurorestorative effects of bone marrow stromal cells in the ischemic rat brain[J]. Glia, 2008, 56(16):1747-1754.
[4] 王凌星, 黄红红, 陈雅芳, 等. 产前应激对成年子代大鼠缺血性卒中后细胞凋亡的影响[J]. 中国应用生理学杂志, 2015, 31(5):427-430.
[5] Coulthard MG, Morgan M, Woodruff TM, et al. Eph/Ephrin signaling in injury and inflammation[J]. Am J Pathol, 2012, 181(5):1493-1503.
[6] Goldshmit Y, Spanevello MD, Tajouri S, et al. Epha4 blockers promote axonal regeneration and functional recovery fo llowing spinal cord injury in mice[J]. PLoS One, 2011, 6(9):e24636.
[7] Parmentier-Batteur S, Finger EN, Krishnan R, et al. Atten-uation of scratch-induced reactive astrogliosis by novel Epha4 kinase inhibitors[J]. J Neurochem, 2011, 118(6):1016-1031.
[8] Igosheva N, Taylor PD, Poston L, et al. Prenatal stress in the rat results in increased blood pressure responsiveness to stress and enhanced arterial reactivity to neuropeptide Y in adulthood[J]. J Physiol, 2007, 582(Pt 2):665-674.
[9] Longa EZ, Weinstein PR, Carlson S, et al. Reversible mid-dle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1):84-91.
[10] Swanson RA, Morton MT, Tsao-Wu G, et al. A semiauto-mated method for measuring brain infarct volume[J]. J Cereb Blood Flow Metab, 1990, 10(2):290-293.
[11] 尚宇, 吕红梅, 顾佩菲. 异丙酚对局灶性脑缺血/再灌注星形胶质细胞GFAP表达的影响[J]. 中国应用生理学杂志, 2009, 25(1):16-17.
[12] Frugier T, Conquest A, McLean C, et al. Expression and activation of Epha4 in the human brain after traumatic injury[J]. J Neuropathol Exp Neurol, 2012, 71(3):242-250.
[13] Goldshmit Y, Bourne J. Upregulation of Epha4 on astrocytes potentially mediates astrocytic gliosis after cortical lesion in the marmoset monkey[J]. J Neurotrauma, 2010, 27(7):1321-1332.
[14] Sofroniew MV. Molecular dissection of reactive astrogliosis and glial scar formation[J]. Trends Neurosci, 2009, 32(12):638-647.
[15] Asher RA, Morgenstern DA, Fidler PS, et al. Neurocan is upregulated in injured brain and in cytokine-treated astrocytes[J]. J Neurosci, 2000, 20(7):2427-2438.
[16] Deguchi K, Takaishi M, Hayashi T, et al. Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain[J]. Brain Res, 2005, 1037(1-2):194-199.

产前应激对子代大鼠脑缺血/再灌注后星形胶质细胞的影响

The effects of prenatal stress on the astrocytes after cerebral ischemia/reperfusion injury in adult offspring rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价

[1]	杨美丽, 洪姗燕, 黄红红, 吕国荣, 王凌星. 大鼠产前手机辐射对子代小脑白质的影响[J]. 中国应用生理学杂志, 2020, 36(1): 77-81.
[2]	董媛媛, 王琳, 褚旭, 崔帅, 孔庆霞. 鞘氨醇激酶1和1-磷酸鞘氨醇受体2在癫痫大鼠海马组织中表达的变化^*[J]. 中国应用生理学杂志, 2019, 35(4): 308-311.
[3]	王凌星, 黄红红, 吕国荣. 产前850-1900MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响[J]. 中国应用生理学杂志, 2018, 34(1): 35-38.
[4]	司艺玲, 张金英, 邓子辉, 薛辉, 颜光涛. 瘦素对小鼠脑缺血/再灌注损伤神经元凋亡的影响[J]. 中国应用生理学杂志, 2016, 32(4): 305-309.
[5]	康文博, 李晓红, 陈羽中, 王景景, 涂悦, 张赛, 梁海乾. 亚低温对脊髓损伤后反应性星形胶质细胞增生的影响[J]. 中国应用生理学杂志, 2016, 32(4): 289-292.