有氧运动同时补充玉米肽对肥胖大鼠脂肪分解关键酶的影响

doi:10.13459/j.cnki.cjap.2016.04.010

摘要/Abstract

摘要： 目的：研究有氧运动同时补充玉米肽对高脂饮食诱导的肥胖大鼠减脂的作用及其与脂肪分解关键酶甘油三酯脂肪酶（ATGL）和脂蛋白酯酶（LPL）关系。方法：4周龄健康雄性SD大鼠150只，体重160~180 g，随机选取15只作为普通膳食不运动组，给予普通饲料喂养。剩余135只大鼠进行8周的高脂饲料喂养建立肥胖大鼠模型，以体重超过普通膳食不运动组大鼠平均体重的20%作为肥胖大鼠建模成功的标准。将建模成功的肥胖大鼠40只随机分为5组（n=8）：肥胖对照组、酪蛋白组、玉米肽组、运动组和运动+玉米肽组。除酪蛋白组、玉米肽组喂养自制饲料外，其余各组均用普通饲料喂养，运动组每天进行15 m/min，持续时间60 min的跑台运动，每周6天。4周运动和玉米肽干预后取血，检测大鼠血浆中TG、TC、HDL、LDL的含量；取大鼠肾周、附睾脂肪和肝，检测肾周和附睾脂肪的重量，Western blot检测大鼠肝ATGL、脂肪LPL的蛋白表达水平。结果：与肥胖对照组大鼠相比：①运动组、运动+玉米肽组大鼠的体重、附睾和肾周脂肪含量明显降低（P<0.05），且运动+玉米肽组比运动组下降得更明显（P<0.05），而其它组大鼠无显著差异。②运动组大鼠血浆TG显著降低，运动+玉米肽组的血浆TG、TC显著降低（P<0.05），其它组大鼠的TG、TC无显著差异；血浆HDL和LDL各组间均无显著性差异。③运动组和运动+玉米肽组大鼠的肝ATGL、脂肪组织LPL的蛋白水平明显增加（P<0.01），且运动+玉米肽组比运动组的更显著（P<0.05）；其他两组无显著差异。结论：有氧运动、有氧运动同时补充玉米肽都可以明显降低大鼠的体脂和血脂水平，且后者的作用更强，这可能与其更显著地增加肥胖大鼠肝ATGL和脂肪LPL的蛋白水平有关。而仅仅补充玉米肽不能降低大鼠的体脂和血脂水平。

关键词: 玉米肽, 有氧运动, ATGL, LPL, 血脂

Abstract: Objective:To investigate the effects of supplement of corn peptides plus aerobic exercise on fat loss and blood lipid profile in obese rats resulted from high fat diet and the mechanism:the role of adipose triglyceride lipase (ATGL) and lipoprotein lipase (LPL). Methods:One hundred and fifty male SD rats (4 weeks age) were randomly divided into control group (C, n=15) fed with ordinary diet and obese model group (M, n=135) fed with high fat diet for 8 weeks. Forty obese rats whose body weight increased by 20% of the mean value of con-trol group were selected and randomly divided into 5 groups(n=8):obesity control group, casein group, corn peptides group, exercise group and exercise+corn peptides group. The rats of the latter two groups completed aerobic excise for 4 weeks at speed of 15 m/min and duration of 60 min per time (6 times/week). After 4 weeks of intervention, the bloods of rats were collected and blood lipid profile plasma levels of triglyceride(TG), total cholesterol (TC), high density lipoprotein(HDL), low density lipoprotein(LDL) were detected. The perirenal fat and epididymal fat were collected and weighed, and the protein levels of ATGL in livers and LPL in adipose tissues were detected by Western blot. Results:①Compared with obesity control group, the body weight and the masses of perirenal fat and epididymal fat in exercise group and corn peptides+exercise group were decreased obviously, with more obvious decrease in the corn peptides+exercise group compared with the exer-cise group. No difference was found in the rats of corn peptides group and casein group. ②Compared with obesity control group, the plasma TG was decreased in exercise group, and the plasma levels of TG and TC were reduced in the rats of corn peptides+exercise group, while they remained unchanged in the rats of corn peptides group and casein group. The plasma levels of HDL and LDL had no significant difference a-mong groups. ③The protein levels of ATGL in livers and LPL in adipose tissues were significantly increased in the rats of exercise group and corn peptides+exercise group, with more obvious in the latter group. No difference was shown in the rats of corn peptides group and casein group compared with obesity control group. Conclusion:Significant decrease of body weight, perirenal fat and epididymal fat as well as plasma TG, TC were induced by 4-week aerobic exercise or supplement of corn peptides plus exercise, with more obvious effects induced by corn pep-tides plus exercise than exercise, while no effect was induced by corn peptides alone, which may be related to the enhancements of the protein levels of ATGL in livers and LPL in adipose tissues.

Key words: corn peptides, aerobic exercise, ATGL, LPL, blood lipid profile

鲁林, 刘桂, 王晓慧, 娄淑杰. 有氧运动同时补充玉米肽对肥胖大鼠脂肪分解关键酶的影响[J]. 中国应用生理学杂志, 2016, 32(4): 326-331.

LU Lin, LIU Gui, WANG Xiao-hui, LOU Shu-jie. Effects of supplement of corn peptides combined with aerobic exercise on lipolysis key enzymes:adipose triglyceride lipase and lipoprotein lipase of obese rats[J]. CJAP, 2016, 32(4): 326-331.

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