The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats

doi:10.13459/j.cnki.cjap.2016.06.005

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (6): 504-508.doi: 10.13459/j.cnki.cjap.2016.06.005

The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats

Ying WANG^1,2, Hong-sheng BIAN¹, Hang ZHAO¹, Ting-li LI¹

1. Heilongjiang University of Chinese Medicine, Harbin 150040, China;
2. Da Li University, Dali 671000, China

收稿日期:2016-07-24 修回日期:2016-10-24 出版日期:2016-11-28 发布日期:2018-06-19
通讯作者: Ting-li LI,MD,Professor,Heilongjiang University of Chinese Medicine,24 He Ping Street,Harbin 150040,China.Tel:86-451-87266874;E-mail:lilhlj@126.com E-mail:lilhlj@126.com

The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats

Ying WANG^1,2, Hong-sheng BIAN¹, Hang ZHAO¹, Ting-li LI¹

1. Heilongjiang University of Chinese Medicine, Harbin 150040, China;
2. Da Li University, Dali 671000, China

Received:2016-07-24 Revised:2016-10-24 Online:2016-11-28 Published:2018-06-19

摘要/Abstract

摘要： Objective: To observe the influence of heterogeneity on sleep-wake architecture in single-prolonged stress (SPS) animal model. Methods: SPS rats were subdivided into low responders (LR) and high responders (HR) based on their freezing responses to a novel environment. Sleeping time (ST), awakening numbers (AN), brief awakening numbers (bAN) and frequency distribution of sleep bouts were used as observing indicators, single factor variance analysis combined with Dunnett t test were used to compare the differences between control, exposure, LR and HR groups. Results: We found sleeping time was increased only in HR group. Moreover, awakening numbers and brief awakening number increased in exposure group and HR group during the light phase, but not in LR group. The number of sleep bouts for the ranges of 40-80s increased obviously in HR group, but not in exposure and LR group. In addition, there were significant correlation between sleep-related parameters and freezing in HR group, but not in LR group. Conclusion: Heterogeneity existed in SPS model in view of different sleep-wake architectures of SPS rats. Rats in HR group exactly mimicked the freezing response and sleep disorders of PTSD. So HR rats were more appropriate to be used as PTSD-like models, especially when studying sleep disorder in PTSD.

关键词: sleep-wake architecture, heterogeneity, single prolonged stress

Abstract: Objective: To observe the influence of heterogeneity on sleep-wake architecture in single-prolonged stress (SPS) animal model. Methods: SPS rats were subdivided into low responders (LR) and high responders (HR) based on their freezing responses to a novel environment. Sleeping time (ST), awakening numbers (AN), brief awakening numbers (bAN) and frequency distribution of sleep bouts were used as observing indicators, single factor variance analysis combined with Dunnett t test were used to compare the differences between control, exposure, LR and HR groups. Results: We found sleeping time was increased only in HR group. Moreover, awakening numbers and brief awakening number increased in exposure group and HR group during the light phase, but not in LR group. The number of sleep bouts for the ranges of 40-80s increased obviously in HR group, but not in exposure and LR group. In addition, there were significant correlation between sleep-related parameters and freezing in HR group, but not in LR group. Conclusion: Heterogeneity existed in SPS model in view of different sleep-wake architectures of SPS rats. Rats in HR group exactly mimicked the freezing response and sleep disorders of PTSD. So HR rats were more appropriate to be used as PTSD-like models, especially when studying sleep disorder in PTSD.

Key words: sleep-wake architecture, heterogeneity, single prolonged stress

Ying WANG, Hong-sheng BIAN, Hang ZHAO, Ting-li LI. The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats[J]. 中国应用生理学杂志, 2016, 32(6): 504-508.

Ying WANG, Hong-sheng BIAN, Hang ZHAO, Ting-li LI. The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats[J]. CJAP, 2016, 32(6): 504-508.

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The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats

The influence of heterogeneity on the analysis of sleep-wake architecture in the single-prolonged stress rats

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