Changes of microcirculation in healthy volunteers and patients with septic shock in Xining

doi:10.13459/j.cnki.cjap.2016.06.011

中国应用生理学杂志 ›› 2016, Vol. 32 ›› Issue (6): 533-539.doi: 10.13459/j.cnki.cjap.2016.06.011

Changes of microcirculation in healthy volunteers and patients with septic shock in Xining

Si-qing MA¹, Shao-hua PENG², Zong-zhao HE¹, Hao WANG¹, Jing-yuan XU³, Hai-bo QIU³, Xin-hui Li¹, Jun-ming LUO⁴

1. Department of Intensive Care Unit, Qinghai Provincial People's Hospital, Xining, 810007, China;
2. Department of Pathology, Medical College of Hunan Normal University, Changsha, Hunan 410013, China;
3. Department of Intensive Care Unit, Zhongda Hospital of Southeast University, Nanjing, 210009, China;
4. Department of Pathology, Qinghai Provincial People's Hospital, Xining, 810007, China

收稿日期:2016-09-26 修回日期:2016-11-16 出版日期:2016-11-28 发布日期:2018-06-19
通讯作者: Hai-bo QIU,MD,Professor,Department of intensive care unit,Zhongda Hospital of Southeast University,Nanjing 210009,China.E-mail:haiboq2000@163.com;Jun-ming LUO,Department of Pathology,Qinghai Provincial People's Hospital,Xining 810007,China.E-mail:jluo099@163.com E-mail:haiboq2000@163.com;jluo099@163.com

Changes of microcirculation in healthy volunteers and patients with septic shock in Xining

Si-qing MA¹, Shao-hua PENG², Zong-zhao HE¹, Hao WANG¹, Jing-yuan XU³, Hai-bo QIU³, Xin-hui Li¹, Jun-ming LUO⁴

1. Department of Intensive Care Unit, Qinghai Provincial People's Hospital, Xining, 810007, China;
2. Department of Pathology, Medical College of Hunan Normal University, Changsha, Hunan 410013, China;
3. Department of Intensive Care Unit, Zhongda Hospital of Southeast University, Nanjing, 210009, China;
4. Department of Pathology, Qinghai Provincial People's Hospital, Xining, 810007, China

Received:2016-09-26 Revised:2016-11-16 Online:2016-11-28 Published:2018-06-19

摘要/Abstract

摘要： Objective: The purpose of this study is to investigate the characteristic of microcirculation in healthy volunteers and patients with septic shock in both Xining (2 260 m) and Nanjing (10 m). Methods: A total of 62 cases, 33 healthy volunteers, 22 cases in Xining, (2 260 m above sea level) and 11 cases in Nanjing (10 m above sea level); and 29 septic shock, 13 cases in Xining and 16 cases in Nanjing were collected. The total vessel density (TVD), perfused vessel density (PVD), proportion of perfused vessel (PPV) and microcirculation flow index (MFI) of both healthy volunteers and septic shock had been investigated by using sidestream dark field (SDF). Analyzed and managed the image data by using AVA3.0 software. Results: In the healthy volunteers in Xining area (22 cases),the volume of TVD (15.59 ±2.58 mm/mm²), PVD (15.58 ±2.58 mm/mm²) and PPV(96.60% ±4.63%) were significant higher than the volume of TVD (10.0 ±2.10 mm/mm²), PVD (10.81 ±2.38 mm/mm²) and PPV (84.24% ±8.00%) of the volunteers (11 cases) in Nanjing (11 cases). But the MFI (2.17 ±0.31) of the healthy volunteers in the Xining was significant lower (P<0.05) than the MFI (3.21 ±0.34) in the healthy volunteers of Nanjing. In the septic shock group (13 cases) in the Xining, the volume of TVD (5.44 ±1.94 mm/mm²), PVD (4.18 ±1.61 mm/mm²), PPV (42.14%±5.38%) and MFI (1.05 ±0.32) compared with the volume of the healthy volunteers in Xining, the TVD (15.59 ±2.58 mm/mm²), PVD (5.58 ±2.58 mm/mm²), PPV (96.60% ±4.63%) and MFI (2.17 ±0.30) were significant lower (P<0.05). In the healthy volunteers compare with septic shock group in Nanjing area, the TVD(6.80±1.72 vs 10.00±2.10, P<0.05), PVD(5.86±1.58 vs10.81±2.38,P<0.05), PPV(45.42±4.86 vs 84.24±4.86, P<0.05), MFI(1.28±0.28 vs 3.21±0.34 P<0.05), there was significant decreased. In the septic shock group in the Xining compared with the septic shock in Nanjing, there was no significant difference. 10 of 13 patients with septic shock were survived in Xining. 13 of 16 patients with septic shock were survived in Nanjing. Conclusion: The changes of physiological and pathophysiological characteristic in microcirculation induced by hypoxia would be useful for clinical treatment of septic shock at high altitude.

关键词: hypoxia, high altitude, microcirculation, septic shock, healthy volunteers

Abstract: Objective: The purpose of this study is to investigate the characteristic of microcirculation in healthy volunteers and patients with septic shock in both Xining (2 260 m) and Nanjing (10 m). Methods: A total of 62 cases, 33 healthy volunteers, 22 cases in Xining, (2 260 m above sea level) and 11 cases in Nanjing (10 m above sea level); and 29 septic shock, 13 cases in Xining and 16 cases in Nanjing were collected. The total vessel density (TVD), perfused vessel density (PVD), proportion of perfused vessel (PPV) and microcirculation flow index (MFI) of both healthy volunteers and septic shock had been investigated by using sidestream dark field (SDF). Analyzed and managed the image data by using AVA3.0 software. Results: In the healthy volunteers in Xining area (22 cases),the volume of TVD (15.59 ±2.58 mm/mm²), PVD (15.58 ±2.58 mm/mm²) and PPV(96.60% ±4.63%) were significant higher than the volume of TVD (10.0 ±2.10 mm/mm²), PVD (10.81 ±2.38 mm/mm²) and PPV (84.24% ±8.00%) of the volunteers (11 cases) in Nanjing (11 cases). But the MFI (2.17 ±0.31) of the healthy volunteers in the Xining was significant lower (P<0.05) than the MFI (3.21 ±0.34) in the healthy volunteers of Nanjing. In the septic shock group (13 cases) in the Xining, the volume of TVD (5.44 ±1.94 mm/mm²), PVD (4.18 ±1.61 mm/mm²), PPV (42.14%±5.38%) and MFI (1.05 ±0.32) compared with the volume of the healthy volunteers in Xining, the TVD (15.59 ±2.58 mm/mm²), PVD (5.58 ±2.58 mm/mm²), PPV (96.60% ±4.63%) and MFI (2.17 ±0.30) were significant lower (P<0.05). In the healthy volunteers compare with septic shock group in Nanjing area, the TVD(6.80±1.72 vs 10.00±2.10, P<0.05), PVD(5.86±1.58 vs10.81±2.38,P<0.05), PPV(45.42±4.86 vs 84.24±4.86, P<0.05), MFI(1.28±0.28 vs 3.21±0.34 P<0.05), there was significant decreased. In the septic shock group in the Xining compared with the septic shock in Nanjing, there was no significant difference. 10 of 13 patients with septic shock were survived in Xining. 13 of 16 patients with septic shock were survived in Nanjing. Conclusion: The changes of physiological and pathophysiological characteristic in microcirculation induced by hypoxia would be useful for clinical treatment of septic shock at high altitude.

Key words: hypoxia, high altitude, microcirculation, septic shock, healthy volunteers

Si-qing MA, Shao-hua PENG, Zong-zhao HE, Hao WANG, Jing-yuan XU, Hai-bo QIU, Xin-hui Li, Jun-ming LUO. Changes of microcirculation in healthy volunteers and patients with septic shock in Xining[J]. 中国应用生理学杂志, 2016, 32(6): 533-539.

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Changes of microcirculation in healthy volunteers and patients with septic shock in Xining

Changes of microcirculation in healthy volunteers and patients with septic shock in Xining

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