有氧运动联合褪黑素对Ⅱ型糖尿病大鼠骨质疏松的影响

doi:10.12047/j.cjap.5395.2017.062

摘要/Abstract

摘要： 目的：观察有氧运动和褪黑素对Ⅱ型糖尿病大鼠骨质疏松的影响。方法：6周龄的成年雌性SD大鼠60只，随机分为安静对照组（N组）10只和Ⅱ型糖尿病模型组50只，N组大鼠不加任何干预，Ⅱ型糖尿病模型组大鼠一次性腹腔注射35 mg/kg链脲佐菌素（STZ），1周后检测大鼠血糖大于16.7 mmol/L为Ⅱ型糖尿病造模成功，将40只成模大鼠随机分为糖尿病对照组（D）、糖尿病+有氧运动组（DE）、糖尿病+褪黑素组（DM）、糖尿病+有氧运动+褪黑素组（DEM），每组10只；DE组和DEM组大鼠采用20 min的递增负荷的方式进行跑台有氧运动，训练持续6周，DM组和DEM组大鼠每天灌胃40 mg/kg褪黑素，观察各组大鼠体重、脊椎骨以及左右股骨骨密度（BMD）、观察大鼠血糖、血清丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、血清总钙（Ca）、无机磷（P）和甲状旁腺素（PTH）的变化。结果：与N组相比，D组大鼠体重、血清SOD、GSH-Px水平、血Ca、腰椎和左右股骨BMD显著降低（P < 0.05，P < 0.01），血糖、血清MDA和血PTH水平显著升高（P < 0.01），血P无明显变化（P > 0.05）；与D组比较，DE组、DM组大鼠大鼠体重、血清SOD、GSH-Px水平、血Ca、腰椎和左右股骨BMD显著升高（P < 0.05，P <0.01），血糖、血清MDA和血PTH水平显著降低（P < 0.05，P < 0.01），血P无明显变化（P > 0.05），有氧运动和褪黑素同时干预效果更好。结论：有氧运动和褪黑素均能改善糖尿病骨质疏松，且两者联合干预的效果更加显著，其可能与通过提高糖尿病大鼠的抗氧化应激能力，调节糖的代谢从而有效地降低血钙和PTH，改善BMD来缓解骨质疏松有关。

关键词: 有氧运动, 褪黑素, 二型糖尿病, 骨质疏松

Abstract: Objective: To investigate the effects of aerobic exercise and melatonin on osteoporosis in type 2 diabetic rats. Methods: Sixty fe male SD rats were randomly divided into the control group (n=10) and the diabetic model group (n=50). The rats in the control group(N group) did not receive any intervention, and the rats in the type Ⅱ diabetic group (D) was injected with streptozocin(STZ) at the dose of 35 mg/kg by intraperitoneally. One week later, rats with blood glucose greater than 16.7 mmol/L were successfully modeled for type Ⅱ diabetes. Forty diabetic rats were randomly divided into diabetic control group (D), diabetes + aerobic exercise group(CDE),diabetes + melatonin group (DM), diabetes mellitus + aerobic exercise + melatonin group (DEM), 10 rats in each group. The rats in DE and DEM groups were treated with 20 min aerobic exercise for 6 weeks. The rats in DM group and DEM group were given 40 mg/kg of melatonin per day. The body weight, vertebrae and left and right femur bone mineral density (BMD) of the rats in each group were observed. Malondialdehyde(MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), serum total calcium (Ca), inorganic phosphate (P) and parathyroid hormone (PTH) were detected. Results: Compared with N group, body weight, serum SOD, GSH-Px levels, blood Ca, lumbar and femoral BMD of rats in group D were decreased significantly (P < 0.05 or P < 0.01). The levels of blood glucose, serum MDA and serum PTH were increased sig-nificantly (P < 0.01), the level of blood P had no changes (P > 0.05); compared with group D, DE group, DM group rats body weight, serum SOD, GSH-Px levels, blood Ca, lumbar spine and femur about BMD were increased significantly (P < 0.05 or P < 0.01). The levels of blood glucose, serum MDA and serum PTH were decreased significantly (P < 0.05 or P < 0.01), the level of blood P had no changes (P > 0.05). The effects in diabetic rats treated with aerobic exercise and melatonin at the same time were much better. Conclusion: Both aero-bic exercise and melatonin can improve diabetic osteoporosis, and the effect of both joint intervention is more significant, it may be associated with oxidative stress by increasing the ability of diabetic rats, regulate glucose metabolism in order to effectively reduce the calcium and PTH, improve BMD to alleviate osteoporosis.

Key words: aerobic exercise, melatonin, Type II diabetes, osteoporosis

景会锋, 王小梅. 有氧运动联合褪黑素对Ⅱ型糖尿病大鼠骨质疏松的影响[J]. 中国应用生理学杂志, 2017, 33(3): 252-256.

JING Hui-feng, WANG Xiao-mei. Effects of aerobic exercise combined with melatonin on osteoporosis of type II diabetic rats[J]. CJAP, 2017, 33(3): 252-256.

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