一次性力竭运动对大鼠PHB1表达及线粒体功能的影响

doi:10.12047/j.cjap.5601.2017.129

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (6): 544-549.doi: 10.12047/j.cjap.5601.2017.129

一次性力竭运动对大鼠PHB1表达及线粒体功能的影响

方雯^1,2, 李泽³, 刘晓华², 柳昭明¹, 敦艺¹, 冯红¹

1. 天津体育学院, 天津 300381;
2. 军事医学科学院卫生学环境医学研究所, 天津 300050;
3. 北京朝阳中西医结合急诊抢救中心, 北京 100020

收稿日期:2017-02-15 修回日期:2017-10-18 出版日期:2017-11-28 发布日期:2018-06-19
基金资助:
国家自然科学基金资助项目（31470061）；天津市自然科学基金项目（14JCZDJC32700）

Effects of exhaustive exercise on the expression of PHB1 and the function of mitochondria in rats

FANG Wen^1,2, LI Ze³, LIU Xiao-hua², LIU Zhao-ming¹, DUN Yi¹, FENG Hong¹

1. Tianjin University of Sport, Tianjin Key Laboratory of Physiology, Tianjin 3000381;
2. Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050;
3. Beijing Chaoyang Emergency Rescue Center Combined with Traditional Chinese Medicine and Western Medicine, Beijing 100020, China

Received:2017-02-15 Revised:2017-10-18 Online:2017-11-28 Published:2018-06-19
Contact: 冯红,Tel:18622351885;E-mail:hong_feng2009@126.com E-mail:hong_feng2009@126.com
Supported by:
国家自然科学基金资助项目（31470061）；天津市自然科学基金项目（14JCZDJC32700）

摘要/Abstract

摘要： 目的：观察一次性力竭运动后大鼠脑、心、骨骼肌组织和线粒体中PHB1含量的变化及对大鼠线粒体功能的影响，探寻PHB1与线粒体功能和能量代谢的关系。方法：健康雄性SD大鼠40只，随机分为2组（n=20）：对照组和一次性力竭运动组，大鼠进行一次性急性跑台运动建立力竭运动模型。收集各组大鼠的心、脑和骨骼肌组织样品并提取线粒体，检测其呼吸功能和ROS的变化。用Western blot方法检测组织和线粒体中PHB1蛋白表达水平；用分光光度计检测各器官中ATP含量以及线粒体中复合体V活性（ATP合酶活性）。结果：①一次性力竭运动后脑、心肌、骨骼肌中ATP含量显著性降低；②一次性力竭运动后脑、心肌、骨骼肌线粒体中复合体V活性、RCR、ROS显著性降低，ST4均显著性升高，ST3无显著性差异。③一次性力竭运动后心、脑、骨骼肌线粒体中PHB1的表达显著性减少。④通过相关性分析得出：一次性力竭运动后心、脑、骨骼肌中ATP含量与心、脑、骨骼肌中复合体V活性呈正相关；心、脑、骨骼肌中ATP含量和心、脑骨骼肌中PHB1的表达呈正相关。结论：一次性力竭运动后，降低线粒体氧化磷酸化功能，使大鼠脑、骨骼肌线粒体内ROS生成增加，PHB1的表达、ATP含量和复合体V活性均下降。一次性力竭运动使得大鼠线粒体内PHB1表达降低，线粒体功能减弱，机体能量代谢降低。

关键词: 一次性力竭运动, 线粒体, 线粒体呼吸功能, PHB1:能量代谢, 大鼠

Abstract: Objective: To observe the changes of Prohibitin1(PHB1) contents in rat brain, heart, skeletal muscle tissue and mitochondria with acute exhaustive exercise and the effects of acute exhaustive exercise on mitochondrial function in rats, to explore the relationship among PHB1 and mitochondrial function and energy metabolism.Methods: Acute exhaustive exercise model:The rats carried acute exhaustive exercise after 8 weeks of feeding. The heart, brain and skeletal muscle samples were collected and the mitochondria were collected to detect the changes of respiratory function and reactive oxygen species(ROS). The expression of PHB1 protein in tissues and mitochondria was detected by Western blot. The ATP content in the organs and the activity of complexes (ATP synthase activity) in mitochondria were measured by spectrophotometer.Results: ① The ATP contents of brain, myocardium and skeletal muscle were decreased significantly after acute exhaustive exercise. ②The activities of complex V, respiratory control rates (RCR) and ROS in mitochondria of brain, myocardium and skeletal muscle were decreased significantly after acute exhaustive exercise, respiration rate state 4(ST4) was increased significantly, at the same time, respiration rate state 3(ST3) had no significant difference. ③ The expression of PHB1 in mitochondria of skeletal muscle was decreased significantly after acute exhaustive exercise, while there was no significant change in PHB1 in myocardial tissue and mitochondria. ④ The correlation analysis showed that the ATP contents in the brain, myocardium and skeletal muscle were positively correlated with the activity of complex V and the expression of PHB1 after acute exhaustive exercise.Conclusion: After acute exhaustive exercise, the mitochondrial oxidative phosphorylation was reduced, the ROS production was increased, the expression of PHB1 was decreased, the ATP content and the activity of complex V were decreased in the brain and skeletal muscle of rats. Acute exhaustive exercise reduced the expression of PHB1 in mitochondria, decreased mitochondrial function, and reduced energy metabolism.

Key words: acute exhaustive exercise, tissue, mitochondria, mitochondrial respiratory function, PHB1:energy metabolism, rats

方雯, 李泽, 刘晓华, 柳昭明, 敦艺, 冯红. 一次性力竭运动对大鼠PHB1表达及线粒体功能的影响[J]. 中国应用生理学杂志, 2017, 33(6): 544-549.

FANG Wen, LI Ze, LIU Xiao-hua, LIU Zhao-ming, DUN Yi, FENG Hong. Effects of exhaustive exercise on the expression of PHB1 and the function of mitochondria in rats[J]. CJAP, 2017, 33(6): 544-549.

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一次性力竭运动对大鼠PHB1表达及线粒体功能的影响

Effects of exhaustive exercise on the expression of PHB1 and the function of mitochondria in rats

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