产前850-1900MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响

doi:10.12047/j.cjap.5592.2018.010

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (1): 35-38.doi: 10.12047/j.cjap.5592.2018.010

产前850-1900MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响

王凌星¹, 黄红红¹, 吕国荣^2,3

1. 福建医科大学附属第二医院神经内科, 泉州 362000;
2. 福建医科大学附属第二医院B超室, 泉州 362000;
3. 泉州医学高等专科学校, 泉州 362000

收稿日期:2017-04-17 修回日期:2017-10-31 出版日期:2018-01-28 发布日期:2018-06-19
通讯作者: 吕国荣,Tel:18965525702;E-mail:lxingwan502@gmail.com E-mail:lxingwan502@gmail.com
基金资助:
福建省自然科学基金项目（2015J01449）；福建省卫生计生中青年骨干人才培养项目（2016-ZQN-53）

cts of prenatal exposure of 850-1900MHz mobile phone on the expression of PCNA and DCX in dentate gyrus of offspring rats

WANG Ling-xing¹, HUANG Hong-hong¹, LV Guo-rong^2,3

1. Department of Neurology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China;
2. Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China;
3. Department of Clinical Medicine, Quanzhou Medical College, Quanzhou 362000, China

Received:2017-04-17 Revised:2017-10-31 Online:2018-01-28 Published:2018-06-19
Supported by:
福建省自然科学基金项目（2015J01449）；福建省卫生计生中青年骨干人才培养项目（2016-ZQN-53）

摘要/Abstract

摘要： 目的：探讨产前手机暴露对子代大鼠海马齿状回增殖细胞核抗原（PCNA）和双皮质素（DCX）表达的影响。方法：构建孕鼠手机射频暴露模型，分为对照组、短时暴露组和长时暴露组（n=6），短时和长时暴露组于孕第1-17天分别给予6 h/d和24 h/d的手机通话暴露，观察孕鼠的孕期长短、孕期体重增长和各组的胎儿数、胎儿出生体重。1月龄子代大鼠行焦油紫染色观察海马齿状回细胞形态，免疫组化观察齿状回PCNA和DCX表达，Western blot检测DCX和脑源性神经营养因子（BDNF）表达。结果：各组孕鼠的孕期、妊娠期体重增长和各组的胎儿数、胎儿出生体重无显著差异，长时暴露组子代大鼠的齿状回多形细胞层锥形细胞和DCX阳性细胞出现形态改变。与对照组、短时暴露组比较，长时暴露组子代大鼠齿状回PCNA阳性细胞和DCX、BDNF表达均明显减少（P<0.05）。结论：产前长时手机暴露可能通过改变子代大鼠海马BDNF而影响齿状回的PCNA和DCX表达。

关键词: 产前手机暴露, 子代大鼠, PCNA, DCX

Abstract: Objective: To evaluate the effect of prenatal mobile phone exposure on the expression of proliferating cell nuclear antigen (PCNA) and doublecortin (DCX) in dentate gyrus of offspring rats.Methods: The rat model of prenatal mobile phone exposure was established and there were three groups including control group, short term maternal exposure group and long term maternal exposure group(n=6). From pregnant day 1 to day 17, pregnant rats in long term and short term maternal exposure group were exposed to an mobile phone in talking mode for 6 h/d and 24 h/d, respectively. Length of pregnancy, maternal body weight gain, litter size and pup's body weight were observed. The cell morphology in dentate gyrus of offspring rats at the age of 1 month was studied by cresyl violet staining. The immunohistochemical expression of PCNA and DCX in dentate gyrus of rat offspring were detected, and the expression of DCX and brain derived neurotrophic factor (BDNF) in hippocampus of rat offspring were evaluated by Western blot.Results: There was no difference in length of pregnancy, maternal body weight gain, litter size and pup's body weight among three groups. The morphological changes of pyramidal cells in the polymorphic layer and DCX-positive cells in the dentate gyrus were obvious in rat offspring of long term maternal exposure group. There were less PCNA-positive cells in dentate gyrus and decreased expression of DCX and BDNF in hippocampus by Western blot in long term maternal exposure group compared with control and short term maternal exposure group (all P<0.05).Conclusion: Long term prenatal mobile phone exposure might inhibit the expression of PCNA and DCX in dentate gyrus of rat offspring by down-regulating BDNF.

Key words: prenatal mobile phone exposure, offspring rats, PCNA, DCX

王凌星, 黄红红, 吕国荣. 产前850-1900MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响[J]. 中国应用生理学杂志, 2018, 34(1): 35-38.

WANG Ling-xing, HUANG Hong-hong, LV Guo-rong. cts of prenatal exposure of 850-1900MHz mobile phone on the expression of PCNA and DCX in dentate gyrus of offspring rats[J]. CJAP, 2018, 34(1): 35-38.

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产前850-1900MHz手机暴露对子代大鼠齿状回PCNA和DCX表达的影响

cts of prenatal exposure of 850-1900MHz mobile phone on the expression of PCNA and DCX in dentate gyrus of offspring rats

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