花青素对完全弗氏佐剂诱导慢性炎性痛的镇痛作用及其机制

doi:10.12047/j.cjap.5671.2018.106

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (5): 476-480.doi: 10.12047/j.cjap.5671.2018.106

• 研究论文 • 上一篇

花青素对完全弗氏佐剂诱导慢性炎性痛的镇痛作用及其机制

王静¹, 陈泽峰², 和春霞², 王莉莉², 郑嘉慧², 张皓³, 王占磊⁴, 鲁亚平⁵

1. 皖南医学院基础医学院生理学教研室, 安徽芜湖 241002;
2. 皖南医学院2013级临床专业, 安徽芜湖 241002;
3. 皖南医学院2015级临床专业, 安徽芜湖 241002;
4. 皖南医学院2014级麻醉专业, 安徽芜湖 241002;
5. 安徽师范大学生命科学学院, 安徽芜湖 241002

收稿日期:2018-01-12 修回日期:2018-08-20 出版日期:2018-09-28 发布日期:2019-02-21
通讯作者: 鲁亚平,Tel:0553-3869297;E-mail:50116113@qq.com E-mail:50116113@qq.com
基金资助:
国家自然科学基金面上项目（81671586）；安徽省教育厅高校优秀人才计划项目（gxyq2017036）；皖南医学院重点科研培育基金（WK2016Z10）；皖南医学院中青年基金（WK201507）；安徽省大学生创新创业训练项目（201710368116，201610368065，201510368064）

The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism

WANG Jing¹, CHEN Ze-feng², HE Chun-xia², WANG Li-li², ZHENG Jia-hui², ZHANG Hao³, WANG Zhan-lei⁴, LU Ya-ping⁵

1. Department of Physiology, School of Basic Medical Sciences, Wannan Medical College, Wuhu 241002;
2. 2013 level clinical specialty, Wannan Medical College, Wuhu 241002;
3. 2015 level clinical specialty, Wannan Medical College, Wuhu 241002;
4. 2014 level anaesthetics specialty, Wannan Medical College, Wuhu 241002;
5. College of Life Science, Anhui Normal University, Wuhu 241002, China

Received:2018-01-12 Revised:2018-08-20 Online:2018-09-28 Published:2019-02-21
Supported by:
国家自然科学基金面上项目（81671586）；安徽省教育厅高校优秀人才计划项目（gxyq2017036）；皖南医学院重点科研培育基金（WK2016Z10）；皖南医学院中青年基金（WK201507）；安徽省大学生创新创业训练项目（201710368116，201610368065，201510368064）

摘要/Abstract

摘要： 目的：本研究旨在探索金叶女贞果实花青素对完全弗氏佐剂（CFA）诱导慢性炎性痛的镇痛作用及其可能的中枢机制。方法：雄性SD大鼠30只随机分为三组（n=10）：正常对照组、慢性炎性痛模型组（左后足跖注射100 μl CFA）、花青素治疗组（模型+花青素90 mg·kg^-1，ig，qd）。造模前和术后第1、3、5、7、9、11、13日测量各组大鼠的体重、基础痛阈（热痛阈和机械痛阈），左后肢足趾容积；术后第14日实验结束，分光光度计法测定血清各项生化指标，Western blot检测海马区总辣椒素受体（TRPV1）和磷酸化辣椒素受体（p-TRPV1）的表达。结果：花青素能提高模型组大鼠热痛阈和机械痛阈（P<0.05），降低足趾肿胀度（P<0.05），提高血清SOD水平（P<0.01），降低血清MDA和NO含量（P<0.05），降低大脑海马区p-TRPV1/TRPV1蛋白比例。结论：花青素灌胃14日处理对完全弗氏佐剂诱导的大鼠慢性炎性痛有镇痛作用，其机制可能与降低炎性因子释放，提高抗氧化能力和下调TRPV1磷酸化有关。

关键词: 花青素, 慢性炎性痛, 痛阈, 辣椒素受体

Abstract: Objective: To investigate the effects and mechanisms of anthocyanin from Ligustrum vicaryi on chronic inflammatory pain induced by complete Freund's adjuvant. Methods: Thirty male SD rats were randomly divided into three groups (n=10):normal saline control group (NS), chronic inflammatory pain model group(Mod, injected with complete Freund's adjuvant(CFA) 100 μl to the left hind leg), anthocyanin treatment group(Ant, dosed with anthocyanins (90 m), mechanical pain threshold (MPT), and left toe volume in each group were measured before modeling and 1,3,5,7,9,11,13 days after operation. Antioxidant indexes in serum were mensurated by spectrophotometer, and the total capsaicin receptor (TRPV1) and phosphorylated capsaicin receptor (p-TRPV1) in hippocampus were detected by Western blot. Results: In comparison with controls, HPT and MPT were improved (P<0.05),toe swelling was reduced(P<0.05), the serum level of SOD was increased (P<0.01), while the levels of MDA and NO were decreased (P<0.05), the ratio of P-TRPV1/TRPV1 protein was depressed in Mod rat hippocampal region treated with anthocyanin. Conclusion: The results show that anthocyanins has an analgesic effect on chronic inflammatory pain induced by CFA, and its mechanism may be related to the improvement of antioxidant capacity and the reduction of TRPV1 phosphorylation.

Key words: anthocyanins, chronic inflammatory pain, pain threshold, TRPV1

王静, 陈泽峰, 和春霞, 王莉莉, 郑嘉慧, 张皓, 王占磊, 鲁亚平. 花青素对完全弗氏佐剂诱导慢性炎性痛的镇痛作用及其机制[J]. 中国应用生理学杂志, 2018, 34(5): 476-480.

WANG Jing, CHEN Ze-feng, HE Chun-xia, WANG Li-li, ZHENG Jia-hui, ZHANG Hao, WANG Zhan-lei, LU Ya-ping. The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism[J]. CJAP, 2018, 34(5): 476-480.

参考文献

[1] 张瑛, 王韵. TRPV1:一种同时参与慢性痛外周敏化和疼痛中枢调制的重要分子[J]. 生理学报,2017, 69(5):677-684.
[2] 王星, 张燕玲, 王耘, 等. TRPV1离子通道与中药辛味药性的关系研究[J]. 中国中药杂志, 2014, 39(13):2422-2427.
[3] 龚频, 陈福欣, 金赛, 等. 不同给药方式下蓝莓花青素的镇痛作用研究[J]. 食品研究与开发, 2013, 34(24):186-188.
[4] 王静, 马养民, 逮文静. 蓝莓花青素的纯化及镇痛、抗炎药效学研究[J]. 食品工业科技, 2013, 34(5):338-340.
[5] 高杉, 黄春霞, 黄玲玲, 等. 低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响[J]. 中国临床药理学与治疗学, 2012, 17(5):497-501.
[6] 王静, 王海华, 姜玉新, 等. 金叶女贞果实花青素的镇痛作用初探[J]. 中国临床药理学与治疗学, 2015, 20(1):38-42.
[7] 王静, 王建安, 姜玉新, 等. 金叶女贞果实花青素抗炎镇痛的作用机制[J]. 中国应用生理学杂志, 2015, 31(5):431-436.
[8] 肖婷, 杜俊英, 乐小琴, 等. 外周P2X3受体在不同炎性痛模型中的作用比较[J]. 中国比较医学杂志, 2016, 26(7):74-78.
[9] 邵传森,朱圣禾.临床疾病与免疫[M].北京:人民卫生出版社, 2002:178-179.
[10] 魏春华, 高燕萍, 罗光明, 等. 藏药无尾果抗炎镇痛解热作用研究[J]. 中药材, 2016, 39(12):2875-2878.
[11] Yoshida T, Inoue R, Morii T, et al. Nitric oxide activates TRP channels by cysteine S-nitrosylation[J]. Nat Chem Biol, 2006, 2(11):596-607.
[12] 刘凌云, 吴迪, 李清君, 等. 甲醛炎性痛增强大鼠海马NOS表达和NO生成[J]. 中国应用生理学杂志, 2007, 23(1):30-34.
[13] Tsuchida K, Ibuki T, Matsumura K. Bromoenol lactone, an inhibitor of calcium-independent phospholipase A2, suppresses carrageenan-induced prostaglandin production and hyperalgesia in rat hind paw[J]. Mediators Inflamm,2015, 2015:605-727.
[14] Williams AC, Craig KD. Updating the definition of pain[J]. Pain, 2016, 157(11):2420-2423.
[15] Julius D. TRP channels and pain[J]. Ann Rev Cell Dev Biol, 2013, 29:355-384.
[16] 刘晓丽, 吕翠, 张文生. TRPV1与中药止痛作用研究进展[J]. 中国中药杂志, 2014, 39(10):1757-1760.
[17] 衡立君, 高国栋, 陈军. 辣椒素受体VR1在局部炎症状态下的敏化机制[J]. 神经解剖学杂志, 2004, 20(3):315-318.
[18] Caterina MJ, Leffler A, Malmberg AB, et al. Impaired nociception and pain sensation in mice lacking the capsaicin receptor[J]. Science, 2000, 288(5464):306-313.
[19] Davis JB, Gray J, Gunthorpe MJ, et al. Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia[J]. Nature, 2000, 405(6783):183-187.
[20] Eid SR, Crown ED, Moore EL, et al. HC-030031, a TRPA1 selective antagonist, attenuates inflammatory and neuropathy induced mechanical hypersensitivity[J]. Mol Pain, 2008, 4:48.

花青素对完全弗氏佐剂诱导慢性炎性痛的镇痛作用及其机制

The effects of anthocyanin on chronic inflammatory pain induced by complete Freund's adjuvant and its mechanism

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价