肺腺癌预后相关miRNA生物信息学筛选及其临床意义

doi:10.12047/j.cjap.5755.2018.118

中国应用生理学杂志 ›› 2018, Vol. 34 ›› Issue (6): 530-535.doi: 10.12047/j.cjap.5755.2018.118

肺腺癌预后相关miRNA生物信息学筛选及其临床意义

张皓旻^1,2, 杨波², 陈红飞², 迟小华³, 席义博^1,2, 陈熙勐^1,2, 郭斌^1,2, 贺培凤¹, 卢学春²

1. 山西医科大学管理学院, 太原 030001;
2. 中国人民解放军总医院南楼血液科国家老年疾病临床医学研究中心, 北京 100853;
3. 中国人民解放军火箭军总医院药剂科, 北京 100800

收稿日期:2018-09-25 修回日期:2018-10-26 出版日期:2018-11-28 发布日期:2019-03-09
通讯作者: 卢学春 E-mail:luxuechun@126.com
基金资助:
2017年度国家老年疾病临床医学研究中心招标课题（NCRCG-PLAGH-2017011）；解放军总医院转化医学项目（2017TM-020）

Prognosis-related miRNA bioinformatics screening of lung adenocarcinoma and its clinical significance

ZHANG Hao-min^1,2, YANG Bo², CHEN Hong-fei², CHI Xiao-hua³, XI Yi-bo^1,2, CHEN Xi-meng^1,2, GUO Bin^1,2, HE Pei-feng¹, LU Xue-chun²

1. Management School Shanxi Medical University, TaiYuan 030002;
2. Department of Hematology, Nanlou Division, Chinese PLA General Hospital, National Clinical Research Center for Geriatric Diseases, Beijing 100853;
3. Department of Pharmacy, Chinese PLA Rocket Force General Hospital, Beijing 100800, China

Received:2018-09-25 Revised:2018-10-26 Online:2018-11-28 Published:2019-03-09
Supported by:
2017年度国家老年疾病临床医学研究中心招标课题（NCRCG-PLAGH-2017011）；解放军总医院转化医学项目（2017TM-020）

摘要/Abstract

摘要： 目的：探讨肺腺癌预后相关miRNA组学特征及其临床意义。方法：应用癌症基因组图谱（TCGA）数据库，检索人肺腺癌miRNA表达谱数据，进行差异分析，再利用Cox风险回归模型筛选预后相关miRNA；利用mirwalk分析平台，对筛选出的miRNA进行靶向调控基因预测、KEGG功能富集分析，最后，预测出预后相关miRNA的功能。结果：共筛选肺腺癌差异miRNA46个，其中，上调19个、下调27个；通过Cox生存分析筛选到预后相关miRNA有6个，即hsa-mir-21、hsa-mir-142、hsa-mir-200a高表达，hsa-mir-101、hsa-let-7c、hsa-mir-378e低表达，其中hsa-mir-21、hsa-mir-378e与肺腺癌患者不良预后有关，生存期显著缩短（P<0.05，AUC=0.618）。KEGG分析上述预后相关miRNA靶向调控基因与免疫反应通路、miRNA与癌症通路、代谢通路等有关。结论：hsa-mir-21、hsa-mir-378e与肺腺癌预后不良有关，未来经进一步临床验证有可能作为肺腺癌预后相关的分子标记物。

关键词: 肺腺癌, miRNA, TCGA, 预后, 生物信息学

Abstract: Objective: To investigate the prognosis-related miRNA histological features and clinical significance of lung adenocarcinoma. Methods: Using The Cancer Genome Atlas (TCGA) data, the miRNA expression profile data of human lung adenocarcinoma were searched for differential analysis, and the prognosis-related miRNAs were screened by Cox risk regression model. The targeted miRNAs were predicted by mirwalk analysis platform, KEGG functional enrichment analysis, and finally, predict the function of prognosis-related miRNAs. Results: A total of 46 differential miRNAs in lung adenocarcinoma were screened, including 19 up-regulated and 27 down-regulated. Six prognostic-related miRNAs were screened by Cox survival analysis, namely hsa-mir-21, hsa-mir-142, hsa-mir-200a high expression, hsa-mir-101, hsa-let-7c, hsa-mir-378e low expression, hsa-mir-21 and hsa-mir-378e were associated with poor prognosis in patients with lung adenocarcinoma, and the survival time was shortened significantly (P<0.05, AUC=0.618). KEGG analysis showed that the above prognosis-related miRNA targeting regulatory genes were related with immune response pathways, miRNA and cancer pathways, metabolic pathways and so on. Conclusion: Hsa-mir-21 and hsa-mir-378e are associated with poor prognosis of lung adenocarcinoma, and may be used as a molecular marker for prognosis of lung adenocarcinoma after further clinical verification.

Key words: lung adenocarcinoma, miRNA, TCGA, prognosis, bioinformatics

张皓旻, 杨波, 陈红飞, 迟小华, 席义博, 陈熙勐, 郭斌, 贺培凤, 卢学春. 肺腺癌预后相关miRNA生物信息学筛选及其临床意义[J]. 中国应用生理学杂志, 2018, 34(6): 530-535.

ZHANG Hao-min, YANG Bo, CHEN Hong-fei, CHI Xiao-hua, XI Yi-bo, CHEN Xi-meng, GUO Bin, HE Pei-feng, LU Xue-chun. Prognosis-related miRNA bioinformatics screening of lung adenocarcinoma and its clinical significance[J]. CJAP, 2018, 34(6): 530-535.

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肺腺癌预后相关miRNA生物信息学筛选及其临床意义

Prognosis-related miRNA bioinformatics screening of lung adenocarcinoma and its clinical significance

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