参麦注射液对肠缺血/再灌注肺损伤大鼠肺组织p38MAPK及凋亡相关基因表达的影响

doi:10.12047/j.cjap.5713.2019.016

中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (1): 65-68.doi: 10.12047/j.cjap.5713.2019.016

参麦注射液对肠缺血/再灌注肺损伤大鼠肺组织p38MAPK及凋亡相关基因表达的影响

赵嘉涵,贾雨涵,汤雅婷,林艺鑫,王艳蕾^△

华北理工大学基础医学院生理学系, 河北唐山 063210

收稿日期:2018-06-12 出版日期:2019-01-28 发布日期:2019-06-27
通讯作者: ^△ Tel: 0315-8805520; E-mail: 593195374@qq.com
基金资助:
河北省唐山市科学技术研究与发展计划项目资助课题(09130202A-3-7);华北理工大学博士科研启动基金项目(BS2017074);华北理工大学大学生创新计划项目(X2018198)

Effects of Shenmai injection on the expression of p38MAPK and the apoptosis-related genes in lung injury induced by intestinal ischemia/reperfusion in rats

ZHAO Jia-han, JIA Yu-han, TANG Ya-ting, LIN Yi-xin, WANG Yan-lei^△

Department of Physiology, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, China

Received:2018-06-12 Online:2019-01-28 Published:2019-06-27

摘要/Abstract

摘要： 目的:观察参麦注射液(SM)对肠缺血/再灌注(I/R)肺损伤大鼠肺组织p38MAPK和凋亡相关基因Bax、Bcl-2蛋白表达的影响,探讨其保护机制。方法:采用夹闭肠系膜上动脉(SMA)方法建立大鼠肠I/R损伤模型。24只SD大鼠随机分为对照组(Control组)、肠缺血/再灌注组(I/R组)、参麦注射液组(SM+I/R组),每组8只。比较各组大鼠肺湿/干比(W/D)、肺表面活性物质主要成分卵磷脂(PC)及总磷脂(TPL)含量的变化;同时免疫组织化学法检测各组大鼠肺组织中p38MAPK、Bax及Bcl-2蛋白的表达水平。结果:与对照组比较,I/R组肺组织W/D明显升高,而PC和TPL的含量显著降低,肺组织p38MAPK、Bcl-2和Bax蛋白表达明显增强(P均＜0.01),其中Bax的增强比Bcl-2的增强更为明显,Bcl-2/Bax比值降低(P＜0.01);与I/R组比较,SM+I/R组大鼠肺组织W/D明显降低,PC和TPL的含量增加,肺组织p38MAPK和Bax蛋白表达下降(P均＜0.01),Bcl-2的表达增强,Bcl-2/Bax比值明显升高(P＜0.01)。相关分析显示,肠I/R时肺组织p38MAPK蛋白表达水平与肺表面活性物质主要功能成分PC含量及凋亡基因Bcl-2/Bax比值呈负相关(r分别为-0.787,-0.731,P均＜0.01)。结论:SM可能通过抑制p38MAPK信号通路的激活,提高Bcl-2/Bax比值来阻抑细胞凋亡,从而减轻肠I/R时的肺损伤。

关键词: 参麦注射液, 小肠, 缺血再灌注, 急性肺损伤, p38MAPK, 细胞凋亡, 大鼠

Abstract: Objective:To observe the effects of Shenmai injection(SM) on p38MAPK and the apoptosis-related genes in lung injury induced by intestinal ischemia reperfusion (I/R) in rats and to investigate the protective mechanism of SM. Methods: Rat model of intestinal I/R injury was established with clamping of the superior mesenteric artery (SMA) for 60 min and then clamping was relieved for 60 min. Twenty-four SD rats were randomly divided into three groups with eight rats in each: control group, intestinal ischemia/reperfusion group(I/R group), Shenmai injection treated group (SM+I/R group). Lung wet/dry weight ratio(W/D), the contents of phosphatidylcholine (PC) and total phospholipid(TPL) which are the major ingredients of pulmonary surfactant were measured, as well as the expression levels of p38MAPK, Bcl-2 and Bax proteins in lung tissue were examined by using immunohistochemical method. Results: Compared with control group, lung W/D was significantly increased, the contents of PC and TPL were significantly decreased, the protein expression levels of p38MAPK, Bcl-2 and Bax were significantly increased in I/R group (all P＜0.01). But Bax protein expression was much greater than Bcl-2 protein expression, the ratio of Bcl-2 to Bax were significantly decreased in I/R group than that in control group (P＜0.01). Compared with I/R group, lung W/D was significantly decreased, while the contents of PC and TPL were significantly increased, the p38MAPK and Bax protein expression levels were significantly decreased in SM+I/R group (all P＜0.01); both Bcl-2 protein expression and the ratio of Bcl-2 to Bax were significantly increased in SM+I/R group than those in I/R group (P＜0.01). The correlation analysis indicated that the expression level of p38MAPK protein in lung tissue was negatively correlated with the contents of PC and the ratio of Bcl-2 to Bax (r is -0.787 and -0.731, all P＜0.01). Conclusion: SM can protect the lung injury induced by intestinal I/R injury, which may be mediated by inhibiting the activation of p38MAPK, improving the ratio of Bcl-2 to Bax to inhibit lung apoptosis.

Key words: Shenmai injection, intestine, ischemia/reperfusion, acute lung injury, p38MAPK, apoptosis, rat

赵嘉涵, 贾雨涵, 汤雅婷, 林艺鑫, 王艳蕾. 参麦注射液对肠缺血/再灌注肺损伤大鼠肺组织p38MAPK及凋亡相关基因表达的影响[J]. 中国应用生理学杂志, 2019, 35(1): 65-68.

ZHAO Jia-han, JIA Yu-han, TANG Ya-ting, LIN Yi-xin, WANG Yan-lei. Effects of Shenmai injection on the expression of p38MAPK and the apoptosis-related genes in lung injury induced by intestinal ischemia/reperfusion in rats[J]. CJAP, 2019, 35(1): 65-68.

参考文献

[1] Jia Z, Lian W, Shi H, et al. Ischemic postconditioning protects against intestinal ischemia/reperfusion injury via the HIF-1α/miR-21 axis [J]. Sci Rep, 2017, 7(1): 16190.
[2] Onder A, Kapan M, Gümüs M, et al. The protective effects of curcumin on intestine and remote organs against mesenteric ischemia/reperfusion injury [J]. Turk J Gastroenterol, 2012, 23(2): 141-147.
[3] Tan H, Chen L, Ma J. Penehyclidine hydrochloride post-conditioning reduces ischemia/reperfusion-induced cardiomyocyte apoptosis in rats [J]. Exp Ther Med, 2017, 14(5): 4272-4278.
[4] Yu Y, Liu Q, Guo S, et al. 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin promotes endothelial cell apoptosis through activation of EP3/p38MAPK/Bcl-2 pathway[J]. J Cell Mol Med, 2017, 21(12): 3540-3551.
[5] 黄林静, 张聪聪, 赵美平, 等. MAPK信号通路对大鼠低氧性PASMCs增殖、凋亡的调控[J]. 中国应用生理学杂志, 2017, 33(3): 226-230.
[6] 王艳蕾, 崔国金, 蔡庆艳, 等. 参麦注射液对肠缺血再灌注肺脂质过氧化损伤的影响[J]. 天津医药, 2010, 38(11): 986-989.
[7] 高晓明, 宋建良, 谢翠微, 等. 心钠素与实验性肺水肿[J]. 北京医科大学学报, 1987, 19(2): 69-71.
[8] 李影林. 中华医学检验全书[M]. 北京: 人民卫生出版社, 1996: 737-739.
[9] Zhao L, Gu Q, Xiang L, et al. Curcumin inhibits apoptosis by modulating Bax/Bcl-2 expression and alleviates oxidative stress in testes of streptozotocin-induced diabetic rats [J]. Ther Clin Risk Manag, 2017, 13: 1099-1105.
[10]Cao S, Zhu X, Du L. P38 MAP kinase is involved in oleuropein-induced apoptosis in A549 cells by a mitochondrial apoptotic cascade [J]. Biomed Pharmacother, 2017, 95: 1425-1435.
[11]Zhao HG, Zhou SL, Lin YY, et al. Toxicarioside N induces apoptosis in human gastric cancer SGC-7901 cell by activating the p38MAPK pathway [J]. Arch Pharm Res, 2018, 41(1): 71-78.
[12]Cuenda A, Rousseau S. p38 MAP-kinases pathway regulation, function and role in human diseases [J]. Biochim Biophys Acta, 2007, 1773(8): 1358-1375.
[13]Corre I, Paris F, Huot J. The p38 pathway, a major pleiotropic cascade that transduces stress and metastatic signals in endothelial cells [J]. Oncotarget, 2017, 8(33): 55684-55714.
[14]Ye LF, Zheng YR, Wang LH. Effects of Shenmai injection and its bioactive components following ischemia/reperfusion in cardiomyocytes [J]. Exp Ther Med, 2015, 10(4): 1348-1354.
[15]于齐宏, 杜奕奇, 李兆申, 等. 参麦注射液减轻大鼠重症急性胰腺炎相关性肺损伤[J]. 第二军医大学学报, 2013, 34(11): 1183-1186.
[16]刘小芳. 参麦注射液治疗急性肺损伤的疗效及对血清ICAM-1、ET-1及NO的影响[J]. 现代中西医结合杂志, 2017, 26(25): 2784-2786.
[17]王艳蕾, 景友玲, 赵景霞, 等. 参麦注射液对肢体缺血/再灌注时肺脂质过氧化损伤的防护作用[J]. 中国应用生理学杂志, 2006, 22(1): 13-15.

参麦注射液对肠缺血/再灌注肺损伤大鼠肺组织p38MAPK及凋亡相关基因表达的影响

Effects of Shenmai injection on the expression of p38MAPK and the apoptosis-related genes in lung injury induced by intestinal ischemia/reperfusion in rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	扈敬, 李法东, 邵小婷, 李思葳, 张欣颖, 赵冰冰, 徐长庆, 魏璨. 精胺对高糖引起的大鼠心肌纤维化的影响及其机制[J]. 中国应用生理学杂志, 2020, 36(3): 193-196.
[2]	朱建忠, 赵灿, 隋月林, 刘媛媛, 乔跃兵. 瘦素对糖尿病大鼠糖脂代谢及炎性因子的影响[J]. 中国应用生理学杂志, 2020, 36(3): 197-201.
[3]	孙晓娟, 冯武龙, 侯娜, 李娜, 姜蔚丽. 有氧运动和白藜芦醇对2型糖尿病大鼠肾脏JAK2及TGF-β1的影响[J]. 中国应用生理学杂志, 2020, 36(3): 202-206.
[4]	刘凯丽, 都新新, 张文琴, 吴亚俐, 王茜, 王春芳, 崔香丽. 过表达miR-31对结肠炎模型小鼠TLR4/NF-κB信号通路及凋亡蛋白的调控[J]. 中国应用生理学杂志, 2020, 36(3): 211-215.
[5]	石慧, 梁晓珊, 黄丽文, 罗之刚, 谭龙. 一种高尿酸血症大鼠模型诱导方法的改良和效果评价研究[J]. 中国应用生理学杂志, 2020, 36(3): 223-227.
[6]	杨琼, 潘娅, 陈粲, 戴桃李. 阿尼西坦对血管性痴呆大鼠的治疗作用[J]. 中国应用生理学杂志, 2020, 36(3): 232-234.
[7]	韩洁, 刘子怡, 方振, 田蕾, 杨丹凤, 袭著革, 刘晓华. 重复制动应激对雌性大鼠下丘脑-垂体-卵巢轴的影响[J]. 中国应用生理学杂志, 2020, 36(3): 245-249.
[8]	孙景然, 樊祢祢, 张桢, 吴瑾, 韩殿鹏, 白家磊, 杜连群, 房彦军. 有机磷阻燃剂TDCIPP对雌性大鼠甲状腺的潜在毒性作用[J]. 中国应用生理学杂志, 2020, 36(3): 250-254.
[9]	陈宇峰, 董凡赫, 楼云玮, 寿今豪, 张慧婷, 周一超, 严明, 毛红娇, 张云. 补骨脂素对TCP磨损颗粒所致大鼠成骨细胞损伤的干预作用及其机制[J]. 中国应用生理学杂志, 2020, 36(3): 255-260.
[10]	李家卉, 张敏, 付子豪, 李勇枝, 王佳平, 高峰, 董玲. 经皮穿刺抽取大鼠脑脊液方法的改良[J]. 中国应用生理学杂志, 2020, 36(3): 265-267.
[11]	徐昌顺, 孙培, 林春. 改良型大鼠鞘内穿刺置管针及导管末端注药装置在大鼠鞘内置管术中的应用研究[J]. 中国应用生理学杂志, 2020, 36(3): 283-288.
[12]	王凌星, 洪姗燕, 杨美丽, 黄红红. 丁苯酞对睡眠剥夺大鼠额叶小胶质细胞活化的影响^*[J]. 中国应用生理学杂志, 2020, 36(2): 106-110.
[13]	完建永, 张勇, 刘世强, 华田苗, 孙庆艳. 运动对胰岛素抵抗大鼠肝脏BIM信号通路的影响^*[J]. 中国应用生理学杂志, 2020, 36(2): 115-118.
[14]	姚良雪, 韩家鑫, 魏培韵, 侯思博, 何潇剑, 张瑞岭, 杜爱林. 丁苯酞对慢性酒精中毒大鼠海马、杏仁核H₂S/CBS通路及学习记忆能力的影响^*[J]. 中国应用生理学杂志, 2020, 36(2): 138-142.
[15]	窦丽. 游泳训练对高血压大鼠血压及血栓前状态分子的影响^*[J]. 中国应用生理学杂志, 2020, 36(2): 161-164.