银杏叶提取物对野百合碱诱导的大鼠右心室心肌肥厚的干预作用及其机制*

doi:10.12047/j.cjap.5759.2019.037

摘要/Abstract

摘要： 目的: 探讨银杏叶提取物对大鼠右心室心肌肥厚的干预作用及其机制。方法: 72 只SD 大鼠随机分为3 组,每组24只: 对照组(CON组)、野百合碱诱导右心室心肌肥厚组(MCT组)、银杏叶提取物干预组(EGB组)。MCT组与EGB组首日均以2%MCT按60 mg/kg 剂量腹腔注射,注射后第2日开始,MCT组每日予2 ml 0.9% NaCl灌胃,EGB组以60 mg/kg银杏叶提取物灌胃,对照组SD大鼠首日一次性腹腔注射2 ml 0.9%NaCl注射液。3周后检测各组大鼠心脏血流动力学变化、计算心脏重量指数、HE染色观察心肌病理形态学变化、RT-PCR法检测TRPC6 mRNA表达和Western blot法检测蛋白的表达水平。结果: 与对照组比较,MCT组反映右心室肥厚程度的指标如RVSP、RV±dp/dt_max及RVMI显著增加(P＜0.01),而EGB早期干预组虽然与MCT组的各项指标有相同趋势的变化,但是EGB组各项指标变化的幅度均显著降低(P＜0.01),且EGB组的心肌肥厚指数均显著低于MCT组(P＜ 0.01);HE染色观察心肌形态学变化结果:MCT组呈典型心肌肥厚表现;EGB组右心室心肌细胞较MCT组有显著改善;MCT组及EGB组SD大鼠右心室TRPC6 mRNA及蛋白相对表达水平升高(P＜0.05),而EGB组较MCT组显著降低(P＜0.05)。结论: 银杏叶提取物可能通过降低TRPC6的表达阻碍心肌细胞中CaN／NFAT信号路径而发挥对心肌肥厚的早期保护作用。

关键词: 银杏叶提取物, 大鼠, 野百合碱, 规范性瞬时感受器电位, 心肌肥厚

Abstract: Objective: To study the protective effects of ginkgo biloba extract on the right ventricular hypertrophy. Methods: Seventy-two SD male rats were randomly divided into 3 groups: control group(CON), monocrotaline-induced right ventricular hypertrophy group (MCT) and ginkgo biloba extract treated group (EGB) (n=24 in each group). Group MCT and group EGB were intraperitoneally injected with 2%MCT at the dose of 60 mg /kg on the first day. From the second day, group MCT was injected with 2 ml 0.9% sodium chloride, and 60 mg/kg ginkgo leaf extract was administered to the stomach in group EGB. The control group was injected with 2 ml 0.9% sodium chloride on the first day. After 3 weeks, in each group,cardiac hemodynamic changes were measured, heart weight index was calculated, and myocardial pathological changes were observed by HE staining. The expression of TRPC6 was detected by real-time polymerase chain reaction (real-time -PCR) and Western blot. Results: Compared with the control group, the right ventricular systolic pressure (RVSP) was increased significantly in the MCT group(P＜0.01), the maximum or decline rate of descent (RV±dp/dt_max) of the right ventricle pressure was increased significantly(P＜0.01), while the EGB group had the same trend as all the indexes in the group MCT, but the amplitude of all indicators in group EGB were decreased significantly than those of group MCT(P＜0.01), and the right ventricular hypertrophy index (RVMI) in group EGB was significantly lower than that in group MCT(P＜0.01).Group MCT showed typical myocardial hypertrophy performance by HE staining, and the right ventricular myocytes in group EGB were significantly improved than that in group MCT, and the mRNA and protein expression levels of TRPC6 in the right ventricle of group MCT and group EGB were increased(P＜0.01), while the EGB group was significantly lower than that of the MCT group(P＜0.01). Conclusion: Ginkgo biloba extract may inhibit the signal pathway of CaN / NFAT in cardiac myocytes by reducing the expression of TRPC6 protein, and then play an early protective effect on myocardial hypertrophy.

Key words: ginkgo biloba extract, rat, monocrotaline, transient receptor potential channels, cardiac hypertrophy

陈慧勤, 蔡克锋. 银杏叶提取物对野百合碱诱导的大鼠右心室心肌肥厚的干预作用及其机制^*[J]. 中国应用生理学杂志, 2019, 35(2): 169-172.

CHEN Hui-qin, CAI Ke-feng. The intervention of ginkgo biloba extract on monocrotaline-induced right ventricular hypertrophy in rats and its mechanism[J]. CJAP, 2019, 35(2): 169-172.

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银杏叶提取物对野百合碱诱导的大鼠右心室心肌肥厚的干预作用及其机制^*

The intervention of ginkgo biloba extract on monocrotaline-induced right ventricular hypertrophy in rats and its mechanism

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