DIO基因多态性与有氧耐力的相关性研究*

doi:10.12047/j.cjap.5733.2019.040

摘要/Abstract

摘要： 目的: 研究碘代甲状腺氨酸脱碘酶(DIO)基因多态性与有氧耐力的相关性,寻找与有氧耐力表型相关的分子标记。方法: 应用基质辅助激光解吸电离飞行时间质谱检测技术,对123名中国北方汉族优秀长跑运动员(EEA)与127名中国北方汉族普通大学生(CG)DIO1基因C785T位点及DIO2基因的Thr92Ala和Gly3Asp位点进行解析并分析比较,其中优秀运动员又根据运动等级和运动项目分为国际健将与健将组(43 vs 80),及5/10 km 和马拉松组(92 vs 31)。结果: 在DIO1的C785T位点及DIO2的Thr92Ala位点,各组间基因型和等位基因频率均无显著性差异(P＞0.05);在DIO2的Gly3Asp位点,三种基因型在CG组与国际健将组、CG组与马拉松组间的分布均差异显著(P＜ 0.05),其中TT基因型在CG组中不表达,仅存在于EEA组,但频率很低。DIO2的Thr92Ala及Gly3Asp位点处于强连锁不平衡,CT单体型在男CG组与女CG组、男CG组与男EEA组间分布均差异显著(P＜ 0.05),在男CG组与男健将组、男马拉松组间的分布也均差异显著(P＜0.05),TC单体型则在女CG组与女国际健将组、女5 000 m和10 000 m组间的分布差异显著(P＜0.05)。结论: DIO2基因Thr92Ala及Gly3Asp位点的CT单体型分布具有性别差异,是男子EEA有氧耐力素质的分子标记,可用于男子长跑健将级运动员及马拉松运动员的分子选材,TC单体型则是女子长跑国际健将运动员和5 000 m、10 000 m运动员有氧耐力素质的分子标记。

关键词: DIO, 基因多态性, 有氧耐力, 遗传标记

Abstract: Objective: To study whether polymorphisms in the iodothyronine deiodinase (DIO) gene region contribute to endurance exercise capacity and to validate whether TSHR gene can be used as genetic marks associated with aerobic endurance performance. Methods: Three SNPs (C785T in DIO1 gene regions, Thr92Ala and Gly3Asp in DIO2 gene regions) were selected. The genotypes of the 123 elite long running athletes(EEA) and 127 college students from northern China(CG) were analyzed using the matrix assisted laser desorption ionisation-time of flight mass spectrometry method(MALDI-TOF). The athletes were divided into different groups according to the sports level and the items, which are international masters and masters (43 vs 80), 5/10 km and marathon (92 vs 31). Results: There was no significant difference of the C785T loci in DIO1 gene and the Thr92Ala loci in DIO2 gene between the EEA and the CG(P＞0.05), while at Gly3Asp loci, the frequency distributions of the 3 genotypes were remarkably different in the groups of control and international masters of sports, as well as in the groups of control and marathon athletes(P＜0.05). The genotype TT only existed in EEA not in CG, however, the frequency distribution was very low. The Thr92Ala and Gly3Asp loci of DIO2 gene were in strong linkage disequilibrium. The frequency distributions of the haplotype CT were significantly different in the male CG and the female CG, the male CG and the male EEA(P＜0.05), the male CG and the male masters of sports, as well as in the male CG and the male marathon athletes(P＜0.05). The frequency distributions of the haplotype TC were remarkably higher in the groups of female international masters of sports and female 5 000 m/10 000 m than those in the female CG(P＜0.05). Conclusion: The frequency distributions of the haplotype CT were different in male and female CG, and haplotype CT could be used as a genetic mark associated with aerobic endurance performance of the male EEA, especially for the long running athletes of masters of sports and marathon, while the haplotype CT was associated with the aerobic endurance performance of the female long running athletes of international masters of sports and 5 000 m/10 000 m.

Key words: DIO, polymorphisms, aerobic endurance performance, genetic mark

周文婷, 胡扬. DIO基因多态性与有氧耐力的相关性研究^*[J]. 中国应用生理学杂志, 2019, 35(2): 183-188.

ZHOU Wen-ting, HU Yang. Association between DIO polymorphism and aerobic endurance[J]. CJAP, 2019, 35(2): 183-188.

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DIO基因多态性与有氧耐力的相关性研究^*

Association between DIO polymorphism and aerobic endurance

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