糖尿病大鼠心肌功能的改变及其机制*

doi:10.12047/j.cjap.5808.2019.083

中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (5): 390-392.doi: 10.12047/j.cjap.5808.2019.083

糖尿病大鼠心肌功能的改变及其机制^*

白淑芝^△, 徐娜, 王跃虹, 李鸿珠, 李宏霞

哈尔滨医科大学病理生理学教研室, 哈尔滨 150086

收稿日期:2019-01-11 出版日期:2019-09-28 发布日期:2020-01-02
通讯作者: ^△Tel: 0451-86674548; E-mail: baishuzhi12@163.com
基金资助:
国家自然科学基金青年基金项目(81200160);黑龙江省自然科学基金(面上项目)资助(H2017007)

Changes of myocardial function in diabetic rats and its mechanism

BAI Shu-zhi^△, XU Na, WANG Yue-hong, LI Hong-zhu, LI Hong-xia

Department of Pathophysiology, Harbin Medical University, Harbin 150086, China

Received:2019-01-11 Online:2019-09-28 Published:2020-01-02

摘要/Abstract

摘要： 目的:观察钙敏感受体(CaSR)表达变化在2型糖尿病大鼠心功能降低中的作用。方法:Wistar大鼠随机分成3组:对照组、糖尿病4周和8周组。糖尿病组大鼠给予高糖高脂饮食喂养,4周后腹腔注射链脲佐菌素建立2型糖尿病模型。通过HE染色观察心脏形态学变化,通过超声心动仪检测心脏功能的变化,通过Western blot检测心肌组织CaSR和PKC-α蛋白表达的变化。结果:与对照组相比,糖尿病大鼠心脏收缩和舒张功能降低,心肌组织出现不规则收缩带,且随着病程延长逐渐加重,同时心肌组织CaSR和PKC-α蛋白表达减少。结论:糖尿病大鼠心肌CaSR等蛋白的表达降低,从而引起细胞内钙紊乱,导致心功能下降。

关键词: 钙敏感受体, 2型糖尿病, 心功能, 大鼠

Abstract: Objective: To investigate the role of calcium-sensing receptor (CaSR) in the decrease of cardiac function in type 2 diabetic rats. Methods: Wistar rats were randomly divided into 3 groups including control, diabetic-4 week and diabetic-8 week groups. Rats in the diabetes group were fed with high-glucose and high-fat diet, and intraperitoneal injection of streptozocin (STZ,30 mg/kg) was conducted 4 weeks later to establish a type 2 diabetes model. Cardiac morphological changes were observed by HE staining, cardiac function was detected by echocardiography, and CaSR and PKC-αprotein expressions in cardiac tissue were detected by Western blot. Results: Compared with the control group, the myocardium of diabetic rats showed irregular contraction zone, decreased expression of CaSR protein, increased expression of PKC-α protein, decreased systolic and diastolic functions, and gradually worsened with the prolongation of the course of the disease. Conclusion: Hyperglycemia inhibits the expression of CaSR protein in myocardium of diabetic rats by activating PKC-α, which can cause intracellular calcium disorder and lead to decreased cardiac function.

Key words: calcium-sensing receptor, type 2 diabetes, cardiac function

白淑芝, 徐娜, 王跃虹, 李鸿珠, 李宏霞. 糖尿病大鼠心肌功能的改变及其机制^*[J]. 中国应用生理学杂志, 2019, 35(5): 390-392.

BAI Shu-zhi, XU Na, WANG Yue-hong, LI Hong-zhu, LI Hong-xia. Changes of myocardial function in diabetic rats and its mechanism[J]. CJAP, 2019, 35(5): 390-392.

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糖尿病大鼠心肌功能的改变及其机制^*

Changes of myocardial function in diabetic rats and its mechanism

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