大鼠脑前扣带皮层(ACC)兴奋性与厌恶情绪的行为-电生理学观察*

doi:10.12047/j.cjap.5840.2019.105

摘要/Abstract

摘要： 目的:探讨激活大鼠ACC脑区的阿片受体降低伤害刺激引起厌恶情绪的作用。方法:将实验大鼠随机分为7组,完全弗氏佐剂(CFA)+生理盐水(NS)组,生理盐水(NS)+生理盐水(NS)组,生理盐水(NS)+μ-阿片受体激动剂([DAla², NMe-Phe⁴, Gly-ol⁵]enkephinlin, DAMGO)组,完全弗氏佐剂(CFA)+ 0.01/0.04/0.2/1 μg/μl DAMGO组(n=6)。实验周期为3 d,第1日测量基础值,第2日预先通过ACC区域给药1 μl,然后将0.08 ml完全弗氏佐剂(CFA)注射到大鼠左后脚掌,第3日观察大鼠的CPA反应、缩足反射潜伏期(PWL)和ACC脑区的电活动。结果:①皮下注射CFA的大鼠,注射前与注射后相比,PWL明显减少(P＜0.05);②在笼具痛侧,CFA组大鼠停留的时间明显少于非痛侧(P＜0.05);③在ACC脑区预先注射0.04/0.2/1 μg/μl DAMGO可明显减弱C-CPA反应(P＜0.05);④在ACC脑区预先注射0.04/0.2/1 μg/μl DAMGO可以降低CFA诱发ACC脑区放电频率的增加(P＜0.05)。结论:激活了大鼠ACC脑区上的μ-阿片受体可以降低伤害性刺激诱发的厌恶情绪的发生。

关键词: 前扣带皮层, μ-阿片受体, 在体多通道, 痛厌恶情绪, 大鼠

Abstract: Objective: Increasing activities in anterior cingulate cortex (ACC) can enhance the aversion reactions associated with noxious stimuli. It has been known that opioid receptors activation can trigger endogenous analgesic effect. This study tried to explore whether opioid receptors activation in the ACC region could reduce the aversion associated with noxious stimuli. Methods: The experimental rats were randomly divided into seven groups, Complete Freund's adjuvant (CFA) + normal saline (NS) group, normal saline (NS) + normal saline (NS) group, normal saline (NS) +DAMGO ((DAla², NMe-Phe⁴, Gly-ol⁵)enkephinlin, μ-opioid receptor agonist) group, complete Freund's adjuvant (CFA)+ 0.01/0.04/0.2/1 μg/μl DAMGO group(n=6). The experimental period was three days. The basal value was measured on the first day. The second day, 1 μl was administered through the ACC area, and then 0.08 ml of complete Freund's adjuvant (CFA) was injected into the left hind paw of the rat. CPA response, paw withdrawal reflex latency (PWL) and electrical activity in the ACC brain region of rats were observed on the third day. Results: ①PWL was significantly decreased in rats after CFA was injected into left hind paw compared with post-injection(P＜0.05).② In the pain side of the apparatus, it took rats less residence time than that in the non-pain side. ③ 0.04/0.2/1 μg/μl DAMGO was given before CFA-injection, C-CPA reactions could be revised significantly. ④ Given 0.04/0.2/1 μg/μl DAMGO in the ACC region could decrease the increasing discharge frequency induced by CFA in ACC neurons. Conclusion: The activation of the mu-opioid receptor in the ACC region alleviates the aversion induced by noxious stimulation.

Key words: anterior cingulate cortex (ACC), μ-opioid receptor, multi-channel electrophysiological technique, pain related aversion, rat

中图分类号:

R338.8

关晓雅, 张肖怡, 李振华, 秦霞, 杜振莹, 赵欣, 李建国, 张策, 张宇. 大鼠脑前扣带皮层(ACC)兴奋性与厌恶情绪的行为-电生理学观察^*[J]. 中国应用生理学杂志, 2019, 35(6): 481-485.

GUAN Xiao-ya, ZHANG Xiao-yi, LI Zhen-hua, QIN Xia, DU Zhen-ying, ZHAO Xin, LI Jian-guo, ZHANG Ce, ZHANG Yu. Excitatory and aversive behaviors - electrophysiological observation of anterior cingulate cortex (ACC) in rats[J]. CJAP, 2019, 35(6): 481-485.

参考文献

[1] Williams AC, Craig KD. Updating the definition of pain[J]. Pain, 2016, 157: 2420-2423.
[2] Niederberger E, Resch E, Parnham MJ, et al. Drugging the pain epigenome[J]. Nat Rev Neurol, 2017, 13(7): 434-447.
[3] Dahlke AM, Sable JJ, Andrasik F. Behavioral therapy: emotion and pain, a common anatomical background[J]. Neurological Sciences, 2017, 38(1 Suppl): 157-161.
[4] Johansen JP, Fields HL, Manning BH. The affective component of pain in rodents: direct evidence for a contribution of the anterior cingulate cortex[J]. Proc Nat Acad Sci, 2001, 98(14): 8077-8082.
[5] Bodnar RJ. Endogenous opiates and behavior: 2015[J]. Peptides, 2017, 88: 126-188.
[6] 李振华, 杨洋, 侯苗苗, 等. 在体多通道电生理记录技术在大鼠痛情绪研究中的应用[J]. 中国应用生理学杂志, 2016, 32(1): 41-45.
[7] 付彤童, 徐静, 万燕杰, 等. 痛厌恶情绪对疼痛的影响及其可能机制[J]. 中国疼痛医学杂志, 2016, 22(6): 428-435.
[8] Kalivas PW, Abhold R. Enkephalin release into the ventral tegmental area in response to stress: modulation of mesocorticolimbic dopamine[J]. Brain Research, 1987, 414(2): 339-348.
[9] Zhang Y, Meng X, Li A, et al. Electroacupuncture alleviates affective pain in an inflammatory pain rat model[J]. Eur J Pain, 2012, 16(2): 170-181.
[10]Bannon MJ, Elliott PJ, Alpert JE, et al. Role of endogenous substance P in stress-induced activation of mesocortical dopamine neurones[J]. Nature, 1983, 306(5945): 791-792.
[11]Zhang Y, Li A, Xin J, et al. Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model[J]. Neurochem Res, 2011, 36(10): 1785-1792.
[12]Liu LY, Zhang RL, Chen L, et al. Chronic stress increases pain sensitivity via activation of the rACC-BLA pathway in rats[J]. Exp Neurol, 2019, 313: 109-123.
[13]唐雨龙, 张玉秋. 前扣带皮层NMDA受体-MAPK-CREB通路参与痛厌恶情绪的分子机制[J]. 生理学报, 2017, 69(5): 637-646
[14]Chen W, Ennes HS, McRoberts JA, et al. Mechanisms of μ-opioid receptor inhibition of NMDA receptor-induced substance P release in the rat spinal cord[J]. Neuropharmacology, 2018, 128: 255-268.
[15]朱贺, 马克涛, 李丽, 等. 神经病理痛大鼠DRG神经元GABA_A受体激活电流的变化[J]. 中国应用生理学杂志, 2011, 27(3): 376-379.
[16]Zhang Y, Ji F, Wang G, et al. BDNF activates mTOR to upregulate NR2B expression in the rostral anterior cingulate cortex required for inflammatory pain-related aversion in rats[J]. Neurochem Res, 2018, 43(3): 681-691.
[17]Gorka SM, Fitzgerald DA, de Wit H, et al. Opioid modulation of resting-state anterior cingulate cortex functional connectivity[J]. J Psychopharmacol, 2014, 28(12): 1115-1124.
[18]Navratilova E, Xie JY, Meske D, et al. Endogenous opioid activity in the anterior cingulate cortex is required for relief of pain[J]. J Neurosci, 2015, 35(18): 7264-7271.
[19]Vogt BA, Sikes RW. The medial pain system, cingulate cortex, and parallel processing of nociceptive information[J]. Prog Brain Res, 2000, 122: 223.
[20]Xie CW, Lewis DV. Involvement of cAMP-dependent protein kinase in μ-opioid modulation of NMDA-mediated synaptic currents[J]. J Neurophysiol, 1997, 78(2): 759-766.

大鼠脑前扣带皮层(ACC)兴奋性与厌恶情绪的行为-电生理学观察^*

Excitatory and aversive behaviors - electrophysiological observation of anterior cingulate cortex (ACC) in rats

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	汤娟, 柯洁, 付裕, 何晓伟, 李先伟. Ⅱ型肺泡上皮细胞间质转分化中的Notch-1/ Twist-1信号通路机制^*[J]. 中国应用生理学杂志, 2021, 37(6): 577-583.
[2]	齐书妍, 黄华, 李永坤, 裴林国, 张文娟. 莪术醇对非酒精性脂肪性肝大鼠肝功能和肝纤维化的影响及机制^*[J]. 中国应用生理学杂志, 2021, 37(6): 611-615.
[3]	朱海东, 吕长坤, 马菲菲. 牛磺酸上调基因1(TUG1)对肝纤维化的作用及其机制^*[J]. 中国应用生理学杂志, 2021, 37(6): 616-621.
[4]	吴江立, 王晓影, 代成, 李睿嘉, 张越, 孙佳欢, 李爱英. 归脾汤对大鼠心肌缺血的干预作用^*[J]. 中国应用生理学杂志, 2021, 37(6): 694-698.
[5]	涂容芳, 何振华, 谭小武, 陈哲, 曾赛丽, 刘莎, 贾远航, 李雪花. 辛伐他汀对大鼠肺纤维化及其内皮间质变过程的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 454-459.
[6]	黄燕云, 高洁, 张利娜, 杜相欣, 张雨彤, 郭霞, 郝娜, 李建国, 张宇. 利用全细胞膜片钳技术记录大鼠脑片NMDA电流的研究^*[J]. 中国应用生理学杂志, 2021, 37(5): 459-462.
[7]	丁茂鹏, 韦凌霞, 王志旺, 程小丽, 庞亚蓉. 鳖甲育肝颗粒对复合因素所致肝纤维化大鼠TGF-β1/Smads通路的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 463-467.
[8]	吴经纬, 王一蓉, 陈伟, 刘文锋. 不同训练方式对大鼠骨骼肌纤维类型转化和CaMK II/MEF2传导途径的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 470-474.
[9]	许骥, 方以群, 包晓辰, 袁恒荣, 王楠, 王芳芳. 核辐射对大深度快速上浮脱险致减压病大鼠相关指标的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 486-489.
[10]	张瑜, 路青华, 曹海芳, 张生荣, 王虎德. GPR81激动剂对非酒精性脂肪肝病大鼠胰岛素抵抗的影响[J]. 中国应用生理学杂志, 2021, 37(4): 354-358.
[11]	丁海丽, 黄增浩, 任在方, 孙竹昕, 李俊平, 王瑞元. 针刺对大鼠运动性骨骼肌损伤内质网应激的干预作用及机制[J]. 中国应用生理学杂志, 2021, 37(4): 359-364.
[12]	杨向君, 王羽, E.巴雅日玛, 赵明, S.贺希格扎日嘎拉. 蒙药绍沙-7味丸防治大鼠心肌缺血/再灌注损伤的作用[J]. 中国应用生理学杂志, 2021, 37(4): 380-384.
[13]	郝卯林, 楼国强, 刘秀洁, 钱巍, 王嘉, 周卓琳, 王万铁. 细胞自噬在大鼠缺血/再灌注肺损伤中的调控作用[J]. 中国应用生理学杂志, 2021, 37(4): 385-388.
[14]	刘雪枫, 乔婧, 高建德, 陈正君, 刘雄. 党参总皂苷对TNBS诱导的大鼠溃疡性结肠炎的保护作用及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 397-401.
[15]	耿元文, 林琴琴, 王湘怡, 李若明, 田振军. 间歇运动对心梗大鼠心肌氧化应激和炎症的影响及其机制[J]. 中国应用生理学杂志, 2021, 37(4): 439-444.