有氧运动对高脂饮食小鼠肝脏中CLK2蛋白表达的影响

doi:10.12047/j.cjap.5915.2020.005

摘要/Abstract

摘要： 目的: 探讨有氧运动对高脂饮食小鼠肝脏中Cdc2 激酶(CLK2)蛋白表达及肝脏脂肪含量的影响。方法: 雄性C57/BL6小鼠经正常饮食或高脂饮食16周后,分为正常饮食组、高脂饮食组和高脂饮食+运动组(8周有氧运动),每组10只小鼠。采用免疫印迹方法比较各组小鼠肝脏CLK2蛋白表达;采用油红O染色法比较各组小鼠肝脏脂肪含量;采用实时定量PCR方法比较各组小鼠脂肪代谢相关基因。结果:与正常饮食组小鼠相比,高脂饮食小鼠表现出胰岛素抵抗,肝脏CLK2蛋白含量增加,以及肝脏脂肪积累增加。然而有氧运动可改善高脂饮食小鼠胰岛素抵抗状态,并抑制肝脏中CLK2蛋白增加。结论:有氧运动可降低高脂饮食小鼠肝脏中CLK2蛋白表达,而改善肥胖小鼠肝脏脂肪堆积及代谢紊乱。

关键词: 有氧运动, 高脂饮食, Cdc2激酶, 肝脂肪积累, 小鼠

Abstract: Objective: To investigate the effects of aerobic exercise on Cdc2-like kinase (CLK2) protein expression and the fat content in liver of mice fed with high fat diet. Methods: C57BL/6 mice were distributed in normal diet, high fat diet (fed with highfat diet during 16 weeks) and trained high fat diet group (fed with high-fat diet during 16 weeks and exercised during 8 weeks),10 mice in each group. The expression of CLK2 protein in liver of each group was detected by Western blot. The fat content of liver in each group was detected by oil red O staining, and the relative genes of fat metabolism in each group were evaluated by real-time quantitative PCR.Results: The mice fed with high fat diet showed insulin resistance, the hepatic CLK2 content and fat content were increased compared to the normal diet group. Otherwise, the chronic physical exercise improved insulin resistance state, prevented the increasing of CLK2 in the liver and attenuated hepatic fat accumulation.Conclusion: Aerobic exercise could reduce the expression of CLK2 protein in the liver of mice fed with high fat diet.

Key words: aerobic exercise, high fat diet, Cdc2-like kinase, hepatic fat accumulation, mice

卢漫漫, 陈家耀, 钮鸣宇, 吴卫东. 有氧运动对高脂饮食小鼠肝脏中CLK2蛋白表达的影响[J]. 中国应用生理学杂志, 2020, 36(1): 23-26.

LU Man-man, CHEN Jia-yao, NIU Ming-yu, WU Wei-dong. Effect of aerobic exercise on the expression of CLK2 protein in liver of mice fed with high fat diet[J]. CJAP, 2020, 36(1): 23-26.

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