甘麦大枣汤对抑郁模型大鼠海马神经元突触结构及结构蛋白表达的影响*

doi:10.12047/j.cjap.5939.2020.094

摘要/Abstract

摘要： 目的: 观察甘麦大枣汤对抑郁模型大鼠行为学及单胺递质的影响,并从突触结构及结构蛋白MAP-2与GAP-43表达改变探讨其潜在作用机制。方法: 60只SD大鼠随机分为5组:正常对照组、模型组、氟西汀组(10.8 mg/kg)、甘麦大枣汤高、低剂量组(9.72、4.86 g/kg),每组12只。除对照组外,其余各组大鼠均采用慢性不可预见性温和应激(CUMS)建立抑郁模型,并于造模同时给药组灌胃给药,连续21 d。采用糖水消耗实验和旷场测试评价大鼠抑郁样行为,ELISA法检测海马单胺递质5-HT、NE含量,Golgi染色观察神经元突触损伤情况,免疫组化法和Western blot法检测海马突触结构蛋白MAP-2和GAP-43的表达。结果: 与对照组比较,抑郁模型大鼠糖水偏好度及自主活动评分均显著下降(P＜0.01),海马5-HT、NE含量显著下降(P＜0.01),树突棘缺失明显,同时MAP-2和GAP-43表达均显著下调(P＜0.01);甘麦大枣汤干预后,模型大鼠抑郁样行为明显缓解(P＜0.01),5-HT、NE含量显著升高(P＜0.05),树突棘密度、长度及分枝增加,MAP-2和GAP-43表达显著回升(P＜0.01)。结论: 甘麦大枣汤能改善抑郁模型大鼠的抑郁样行为,升高海马单胺递质含量,其作用可能与上调突触结构蛋白、缓解神经元突触损伤有关。

关键词: 甘麦大枣汤, 抑郁, 海马, 突触, 大鼠

Abstract: Objective: To investigate the effects of Ganmai Dazao Tang on behavior and monoamine neurotransmitters in rats with depression, and to explore its potential mechanism from synaptic structure. Methods: Sixty SD rats were randomly divided into 5 groups: normal control group, model group, fluoxetine group (10.8 mg/kg), Ganmai Dazao Tang high and low dose group (9.72, 4.86 g/kg), 12 rats in each group. Except the control group, the rats in the other groups were all chronically unpredictable mild stress (CUMS) to establish a depression model, and were treated by intragastric administration for 21 days. The depression-like behaviors of rats were evaluated by sucrose consumption test and open field test. The contents of serotonin (5-HT) and norepinephrine (NE) in hippocampus were detected by ELISA. The synaptic damage of neurons was observed by Golgi staining. The synaptic structure protein expression levels of MAP-2 and GAP-43 of hippocampus were detected by Immunohistochemistry and Western blot. Results: Compared with control group, the sucrose preference and autonomic activity scores of the depression model rats were decreased significantly (P＜0.01), the levels of 5-HT and NE in hippocampus were decreased significantly (P＜0.01), and the dendritic spines were absent, and the expressions of MAP-2 and GAP-43 were down-regulated significantly (P＜0.01). After treated with Ganmai Dazao Tang, the depression-like behavior of the model rats was significantly relieved (P＜0.01), and the levels of 5-HT and NE were increased (P＜ 0.05). Dendritic spine density, length and branching were increased, the expressions of MAP-2 and GAP-43 were increased (P＜ 0.01). Conclusion: Ganmai Dazao Tang can improve the depression-like behavior of depression model rats and increase the monoamine neurotransmitter content in hippocampus, which may be related to up-regulation of synaptic structural proteins and relief of synaptic damage in neurons.

Key words: Ganmai Dazao Tang, depression, hippocampus, synapse, rat

中图分类号:

R277.7

郭锐, 秦卫帅, 张双勇, 张淑玲. 甘麦大枣汤对抑郁模型大鼠海马神经元突触结构及结构蛋白表达的影响^*[J]. 中国应用生理学杂志, 2020, 36(5): 444-448.

GUO Rui, QIN Wei-shuai, ZHANG Shuang-yong, ZHANG Shu-ling. Effects of Ganmai Dazao Tang on synaptic structure of hippocampal neurons in depression model rats[J]. CJAP, 2020, 36(5): 444-448.

参考文献

[1] Bode K, Vogel R, Walker J. Health care costs of borderline personality disorder and matched controls with major depressive disorder: a comparative study based on anonymized claims data[J]. Eur J Health Econ, 2017, 18(9): 1125-1135.
[2] Baka J, Csakvari E, Huzian O, et al. Stress induces equivalent remodeling of hippocampal spine synapses in a simulated postpartum environment and in a female rat model of major depression[J]. Neuroscience, 2017, 20(2): 384-397.
[3] Xu C, Ma XM, Chen HB, et al. Orbitofrontal cortex 5-HT2A receptor mediates chronicstress-induced depressive-like behaviors and alterations of spine density and Kalirin7[J]. Neuropharmacology, 2017, 109: 7-17.
[4] Xiao X, Zhang H, Wang H, et al. Neuroprotective effect of amantadine on corticosterone-induced abnormal glutamatergic synaptic transmission of CA3-CA1 pathway in rat's hippocampal slices[J]. Synapse, 2017, 71(12): 58-67.
[5] 马小娟, 赵杰, 冯振宇, 等. 加味甘麦大枣汤对更年期抑郁症患者神经内分泌系统的影响[J]. 中国中药杂志, 2014, 23(12): 4680-4684.
[6] 凌燕, 冼绍祥, 刘树林. 张仲景甘麦大枣汤治疗抑郁症探讨[J]. 中国中医基础医学杂志, 2015, 21(4): 395-397.
[7] 邱美慧, 吴彦, 彭代辉. 抑郁症海马的结构及功能磁共振成像的研究进展[J]. 上海交通大学学报(医学版), 2015, 6: 901-905.
[8] Ardalan M, Wegener G, Polsinelli B, et al. Neurovascular plasticity of the hippocampus one week after a single dose of ketamine in genetic rat model of depression[J]. Hippocampus, 2016, 26(11): 1414-1423.
[9] 赵洪庆, 韩远山, 柳卓, 等. 焦虑性抑郁模型大鼠脑区单胺递质含量与神经因子表达的变化[J]. 中国实验动物学报, 2017, 25(4): 373-379.
[10]李改芬, 赵名, 赵彤, 等. 二甲双胍对慢性应激大鼠抑郁行为的影响[J]. 中国应用生理学杂志, 2019, 35(3): 245-249.
[11]秦竹, 毕秀华, 唐瑶瑶. 甘麦大枣汤对CUMS大鼠行为学及中枢递质5-HT和NE的影响[J]. 辽宁中医杂志, 2013, 3: 563-565.
[12]郭敏, 李刚. 突触可塑性相关蛋白的研究进展[J]. 神经药理学报, 2013, 3(6): 57-64.
[13]刘文锋, 刘少鹏, 傅让, 等. 耐力运动对增龄大鼠脑皮层突触可塑性的影响及相关调控机制[J]. 中国应用生理学杂志, 2019, 35(4): 339-345.
[14]Adrian M, Kusters R, Storm C, et al. Probing the Interplay between Dendritic Spine Morphology and Membrane-Bound Diffusion[J]. Biophys J, 2017, 113(10): 2261-2270.
[15]曹香玲, 吴新贵, 张俊川, 等. 电针治疗对MCAO大鼠运动功能及梗死对侧大脑皮层GAP-43、MAP-2表达的影响[J]. 华中科技大学学报(医学版), 2018, 47(3): 274-279.
[16]Chirwa S, Aduonum A, Pizarro J, et al. Dopaminergic DA1 signaling couples growth-associated protein-43 and long-term potentiation in guinea pig hippocampus[J]. Brain Res Bull, 2005, 64(5): 433-440.
[17]Liu W, Xue X, Xia J, et al. Swimming exercise reverses CUMS-induced changes in depression-like behaviors and hippocampal plasticity-related proteins[J]. J Affect Disord, 2018, 22(7): 126-135.

甘麦大枣汤对抑郁模型大鼠海马神经元突触结构及结构蛋白表达的影响^*

Effects of Ganmai Dazao Tang on synaptic structure of hippocampal neurons in depression model rats

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