桦木酸对人胃癌SGC-7901细胞增殖的影响*

doi:10.12047/j.cjap.6036.2020.131

中国应用生理学杂志 ›› 2020, Vol. 36 ›› Issue (6): 628-632.doi: 10.12047/j.cjap.6036.2020.131

桦木酸对人胃癌SGC-7901细胞增殖的影响^*

陈亚楠¹, 邵淑丽^1,2Δ, 何孟奇¹, 黄鑫¹, 张伟伟^1,2, 张珍珠^1,2

1.齐齐哈尔大学生命科学与农林学院, 齐齐哈尔 161000;
2.抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 齐齐哈尔 161000

收稿日期:2020-03-06 修回日期:2020-11-25 出版日期:2020-11-28 发布日期:2021-03-15
通讯作者: ^△Tel: 13836267458; E-mail: shshl32@163.com
基金资助:
^*齐齐哈尔大学黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201809)

Effect of betulinic acid on proliferation of human gastric cancer SGC-7901 cells

CHEN Ya-nan¹, SHAO Shu-li^1,2Δ, HE Meng-qi¹, HUANG Xin¹, ZHANG Wei-wei^1,2, ZHANG Zhen-zhu^1,2

1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihaer 161000;
2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihaer 161000, China

Received:2020-03-06 Revised:2020-11-25 Online:2020-11-28 Published:2021-03-15

摘要/Abstract

摘要： 目的:利用不同浓度的桦木酸对人胃癌SGC-7901细胞增殖的影响。方法:桦木酸设4个不同浓度(0、10、20、30 μg/ml),并采用常规化疗药物5-Fu处理作为阳性对照,以探究其对细胞增殖的影响。采用台盼蓝拒染法和吉姆萨染色法分别检测桦木酸对人胃癌SGC-7901细胞生长抑制率及克隆形成率;EdU法检测SGC-7901的细胞增殖;利用流式细胞术检测细胞周期, 应用qRT-PCR和Western blot分别检测细胞周期蛋白cyclin D1,cyclin B1的mRNA和蛋白表达水平。结果:不同浓度的桦木酸处理人胃癌SGC-7901细胞48 h后,其细胞生长抑制率显著升高(P＜0.05),克隆形成率和细胞增殖率均明显降低(P＜0.01),且呈剂量和时间依赖性;人胃癌SGC-7901细胞被阻滞在G1/G0期,细胞周期蛋白cyclin D1和cyclin B1的mRNA和蛋白表达量也随桦木酸浓度升高而显著降低(P＜0.01)。且与5-Fu对照组相比,桦木酸浓度为20 μg/ml和30 μg/ml时,细胞增殖能力明显降低,细胞周期被抑制,细胞周期蛋白表达量均明显降低(P ＜0.05)。结论:桦木酸通过下调cyclin B1和cyclin D1基因表达,将人胃癌SGC-7901细胞阻滞在G1/G0期,从而抑制细胞增殖。

关键词: 桦木酸, SGC-7901细胞, 细胞增殖, 细胞周期

Abstract: Objective: Human gastric cancer SGC-7901 cells were treated with betulinic acid(BA)at the concentrations of 0, 10, 20, and 30 μg/ml, and treated with conventional chemotherapeutic drug 5-Fu as a positive control to explore its effect on cell proliferation. Trypan blue and GIEMSA staining method were used to investigate the effect of BA on cell growth inhibition and clone formation. EdU method and flow cytometry were used to explore the proliferation and cell cycle of SGC-7901 cells after treated with BA, respectively. qRT-PCR and Western blot were also applied to determine the mRNA and protein levels of cyclin D1 and cyclin B1. Results: The cell growth inhibition rate was increased after treated with different concentrations of BA in SGC-7901 cells(P＜0.05). After treated for 48 h, BA decreased the clone information and cell proliferation of SGC-7901 cells markedly in dose-and time-dependent manners (P＜0.01). Flow cytometry analysis showed that BA obviously increased the proportion of SGC-7901 cells in G1 phase but decreased the proportion of those in S phase. qRT-PCR and Western blot analysis showed that the mRNA and protein levels of cyclin D1 and cyclin B1 were significantly downregulated by BA at different concentrations(P＜0.01). Compared with the 5-Fu control group, when the concentration of BA was 20 μg/ml and 30 μg/ml, the cell proliferation ability was significantly decreased, the cell cycle was inhibited, and the expression of cyclin was reduced (all P＜0.05). Conclusion: The betulinic acid regulates the proliferation of SGC-7901 cells by inhibiting the expressions of cyclin D1 and cyclin B1, which leads to cell cycle arrest and proliferative inhibition.

Key words: betulinic acid, SGC-7901 cells, cell proliferation, cell cycle

中图分类号:

Q291

陈亚楠, 邵淑丽, 何孟奇, 黄鑫, 张伟伟, 张珍珠. 桦木酸对人胃癌SGC-7901细胞增殖的影响^*[J]. 中国应用生理学杂志, 2020, 36(6): 628-632.

CHEN Ya-nan, SHAO Shu-li, HE Meng-qi, HUANG Xin, ZHANG Wei-wei, ZHANG Zhen-zhu. Effect of betulinic acid on proliferation of human gastric cancer SGC-7901 cells[J]. CJAP, 2020, 36(6): 628-632.

参考文献

[1] Wang SC, Liu YW, Feng YB, et al. A review on curability of cancers: More efforts for novel therapeutic options are needed[J]. Cancers, 2019, 11(11): 1782.
[2] 方玫玫, 杨钰贤, 李舟, 等. 晚期胃癌不同一线化疗方案治疗及生存分析[J]. 转化医学杂志, 2019, 8(2): 89-94.
[3] Ankit S, Divya A, Robin K, et al. Therapeutic applications of betulinic acid nanoformulations[J]. Ann N Y Acad Sci, 2018, 1421(1): 5-18.
[4] Bildziukevich U, Ozdemir Z, Wimmer Z. Recent achievements in medicinal and supramolecular chemistry of betulinic acid and its derivatives[J]. Molecules, 2019, 24(19): 3546.
[5] Zhan XK, Li JL, Zhang S, et al. Betulinic acid exerts potent antitumor effects on paclitaxel-resistant human lung carcinoma cells (H460) via G2/M phase cell cycle arrest and induction of mitochondrial apoptosis[J]. Oncol Lett , 2018, 16(3): 3628-3634.
[6] Sun LK, Cao JY, Chen K, et al. Betulinic acid inhibits stemness and EMT of pancreatic cancer cells via activation of AMPK signaling[J]. Int J Oncol, 2019, 54(1): 98-110.
[7] 祝志鹏, 徐波, 张丽君, 等. 氟尿嘧啶联合TNF-α对人胃癌细胞SGC-7901的抑制作用[J]. 青岛大学学报(医学版), 2018, 54(6): 635-642.
[8] Chen ZY, Chen X, Chen P, et al. Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression[J]. Cell Death Dis, 2017, 8(10): e3092-e3092.
[9] Huang X, Zang YL, Zhang MH, et al. Nuclear factor of κB1 is a key regulator for the transcriptional activation of milk synthesis in bovine mammary epithelial cells[J]. DNA Cell Biol, 2017, 36(4): 295-302.
[10] Wang QQ, Liu G L, Hu CH. Molecular classification of gastric adenocarcinoma[J]. Gastroenterology Res, 2019, 12(6): 275-282.
[11] 杨丹, 颜琼, 罗德兰, 等. 大蒜素对5-氟尿嘧啶治疗肝癌的增效作用及机制[J]. 中国中西医结合杂志, 2019, 39(1): 72-75.
[12] 王根桃, 黄松. 黄芩素对人口腔鳞癌细胞增殖和侵袭的作用及其机制[J]. 中国应用生理学杂志, 2018, 34(6): 536-540.
[13] 王瑜, 柯瑞君, 蒋盼若, 等. 杨芽黄素对前列腺癌细胞凋亡的影响及其机制[J]. 中国应用生理学杂志, 2019, 35(3): 283-288.
[14] Lee D, Lee SR, Kang KS, et al. Betulinic acid suppresses ovarian cancer cell proliferation through induction of apoptosis[J]. Biomolecules, 2019, 9(7): 257.
[15] Cai YL, Zheng YF, Gu JY, et al. Betulinic acid chemosensitizes breast cancer by triggering ER stress-mediated apoptosis by directly targeting GRP78[J]. Cell Death Dis, 2018, 9(6): 636.
[16] Xu T, Pang QY, Wang Y, et al. Betulinic acid induces apoptosis by regulating PI3K/Akt signaling and mitochondrial pathways in human cervical cancer cells[J]. Int J Mol Med, 2017, 40(6): 1669-1678.
[17] Ingham M, Schwartz GK. Cell-cycle therapeutics come of age[J]. J Clin Oncol, 2017, 35(25): 2949-2959.
[18] Zhang T, Zhou Y, Li L, et al. CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes[J]. Development, 2017, 144(2): 305-312.
[19] Ramos-Garcia P, Gil-Montoya JA, Scully C, et al. An update on the implications of cyclin D1 in oral carcinogenesis[J]. Oral Dis, 2017, 23(7): 897-912.

桦木酸对人胃癌SGC-7901细胞增殖的影响^*

Effect of betulinic acid on proliferation of human gastric cancer SGC-7901 cells

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价

[1]	范有寿, 邱志胜, 赵亚萍. 硫氧还蛋白还原酶1(TrxR1)在胃癌中的表达及对胃癌细胞生长的影响^*[J]. 中国应用生理学杂志, 2020, 36(4): 324-329.
[2]	扈敬, 李法东, 邵小婷, 李思葳, 张欣颖, 赵冰冰, 徐长庆, 魏璨. 精胺对高糖引起的大鼠心肌纤维化的影响及其机制[J]. 中国应用生理学杂志, 2020, 36(3): 193-196.
[3]	计红, 邵子益, 薛琳琳, 牛春阳, 詹雪龙, 杨闯, 甄莉, 杨焕民, 李士泽. α-烯醇化酶基因干扰表达对籽鹅卵泡颗粒细胞增殖及凋亡的影响^*[J]. 中国应用生理学杂志, 2020, 36(2): 184-188.
[4]	赵翔宇, 何振宇, 宰守峰. 人参皂苷Rg5对胃癌细胞周期和侵袭的影响及其机制[J]. 中国应用生理学杂志, 2020, 36(1): 51-54.
[5]	颜新建, 李高峰, 唐梅, 杨小平. 下调脂肪酸合成酶表达对膀胱癌UMUC3细胞增殖、迁移及侵袭能力的影响^*[J]. 中国应用生理学杂志, 2019, 35(6): 543-547.
[6]	张其良, 曹文理, 邓全军. 在食管癌细胞增殖过程中蛋白酶激活受体-2(PAR-2)对细胞周期蛋白D1(CyclinD1)的调控机制^*[J]. 中国应用生理学杂志, 2019, 35(6): 559-562.
[7]	黄生辉, 巩婷, 李妍怡. 中风膏对氧糖剥夺/再复氧损伤大鼠海马神经干细胞增殖的影响[J]. 中国应用生理学杂志, 2018, 34(6): 554-557.
[8]	吴涛, 段晓剑, 田宇, 陈海旭, 徐志宏, 刘静, 王卫华, 朱玲玲, 王刚石. 低氘环境对胃癌细胞生长影响的体外实验[J]. 中国应用生理学杂志, 2017, 33(1): 1-5.