筒鞘蛇菰提取物蛇菰多糖对D-半乳糖诱导大鼠肝损伤的保护作用及其机制

doi:10.12047/j.cjap.6057.2021.018

摘要/Abstract

摘要： 目的: 探讨筒鞘蛇菰提取物蛇菰多糖(BIH)对D-半乳糖(D-gal)致大鼠肝损伤的保护作用及机制。方法: 雄性SD大鼠60只,随机分为正常组(Con)、模型组(D-gal)、蛇菰多糖低剂量组(D-gal+50 mg/kg BIH,BIH-L)、蛇菰多糖中剂量组(D-gal+100 mg/kg BIH,BIH-M)、蛇菰多糖高剂量组(D-gal+200 mg/kg BIH,BIH-H),每组12只。除正常组外,其余各组大鼠每天颈背部皮下注射 100 mg/kg 的 D-gal,正常组大鼠每天颈背部皮下注射相同剂量生理盐水;蛇菰多糖各剂量组每天用相应浓度灌胃,正常组和模型组给予等剂量的生理盐水灌胃,共42 d。自动生化分析仪检测血清ALT、AST、DBIL;HE染色检测肝组织的形态学变化;免疫组织化学检测肝细胞凋亡情况,WB检测Caspase-3、Bax、Bcl-2的蛋白表达;用硫代巴比妥酸法测定 MDA 含量,用黄嘌呤氧化酶法测定 SOD 活性。结果: 与正常组比,模型组大鼠血清 ALT 和 AST、DBIL水平显著升高(P＜0.01);蛇菰多糖处理组ALT、 AST、DBIL水平显著低于模型组(P＜0.01);模型组肝细胞凋亡比正常明显增多(P＜0.01),BIH组肝细胞凋亡比模型组明显减少(P＜0.05);模型组Caspase-3、Bax蛋白表达水平和MDA含量比正常组明显升高(P＜0.01),BIH组比模型组明显降低;而BIH组的Bcl-2表达,SOD 活性比模型组明显升高(P＜0.05或 P＜0.01),BIH不同剂量组之间比较,以中剂量效果最好。结论: 蛇菰多糖对肝损伤有保护作用,且其作用机制可能与其降低肝细胞凋亡,抑制肝组织氧化应激发生有关。

关键词: 筒鞘蛇菰, 蛇菰多糖, 肝损伤, D-半乳糖, 大鼠

Abstract: Objective: To investigate the protective effects and mechanisms of the polysaccharide from Balanophora involucrata HK.f (BIH) on liver injury induced by D-galactose in rats. Methods: Sixty male SD rats were randomly divided into 5 groups: the control group (n=12), the D-gal group (n=12), the BIH-L treatment group (D-gal+50 mg/kg BIH, n=12), the BIH-M treatment group (D-gal+100 mg/kg BIH, n=12), and the BIH-H treatment group (D-gal+200 mg/kg BIH, n=12). The rats were injected into the back of the neck with D-gal of 100 mg/kg/d subcutaneously except for the control group. The BIH treatment group were divided into BIH-L group (50 mg/(kg·d)), BIH-M group (100 mg/(kg·d)), and BIH-H group (200 mg/(kg·d)), respectively. The rats in the BIH group were intragastrically administrated with the relative BIH solution, while the rats in the control and D-gal group were treated with saline solution for 42 days. The serum contents of ALT, AST and DBIL c were tested by automatic biochemical analyzer, the content of MDA was determined by thiobarbital acid method and the SOD activity was detected by xanthine oxidase method. Expressions of Caspase-3, Bax, and Bcl-2 in liver were measured by Western blot, and morphological changes by HE staining and immunohistochemistry. Results: The serum contents of ALT, AST and DBIL in the D-gal group were significantly increased compared with those in Con group (P＜0.01) and were decreased in the BIH group as compared with the D-gal group (P＜0.01). Cell apoptosis, the Caspase-3 and Bax levels, and the MDA content in the D-gal group were increased compared with those in the control group (P＜0.01). And BIH treatment could attenuate these effects induced by D-gal. Meanwhile, the Bcl-2 level and SOD activity in the BIH group were increased compared with that in the D-gal group (P＜0.05, 0.01). Conclusion: BIH can protective liver injury through reducing cell apoptosis and inhibiting oxidative stress.

Key words: Balanophora involucrata Hook.f., balanophora involucrate, liver injury, D-gal, rat

中图分类号:

R285.5

张桃, 谢雅, 陈颖, 夏德尧, 赵方毓, 陈显兵, 樊孝琼. 筒鞘蛇菰提取物蛇菰多糖对D-半乳糖诱导大鼠肝损伤的保护作用及其机制[J]. 中国应用生理学杂志, 2021, 37(3): 276-280.

ZHANG Tao, XIE Ya, CHEN Ying, XIA De-yao, ZHAO Fang-yu, CHEN Xian-bing, FAN Xiao-qiong. Protective effect of the polysaccharide from Balanophora involucrata HK.f on liver injury induced by D-galactose in rats and its mechanism[J]. CJAP, 2021, 37(3): 276-280.

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