推拿对慢性应激大鼠抑郁行为的影响及其机制

doi:10.12047/j.cjap.6088.2021.025

摘要/Abstract

摘要： 目的: 探讨推拿对慢性应激大鼠抑郁行为的影响及其作用机制。方法: 制备慢性轻度不可预见性的应激大鼠模型^[1-2],造模21 d后,进行推拿治疗14 d。分组:空白对照组、模型组、推拿组、氟西汀组,每组10只。每日推拿膀胱经重要穴位10 min(间隔2 min,共2次)。通过体质量检测、旷场、糖水消耗实验和水迷宫实验评价抑郁模型大鼠行为学改变情况;蛋白质免疫印迹法(Western blot)法检测大鼠海马及前额叶皮质组织中ERK/P-ERK、BDNF蛋白表达情况。结果: 模型组与空白组比较,大鼠的体质量、旷场、糖水消耗实验和水迷宫数据均显著下降(P＜0.01),P-ERK、BDNF蛋白含量均显著降低(P＜0.01);推拿组和氟西汀组与模型组比较,大鼠的体质量、旷场实验、糖水消耗实验和水迷宫实验数据均显著上升(P＜0.01),推拿组和氟西汀组大鼠海马及前额叶皮质组织中P-ERK、BDNF蛋白含量均显著升高(P＜0.05,P＜0.01),氟西汀组升高更为显著(P＜0.01)。结论: 推拿可上调大鼠海马及前额叶皮质组织中ERK蛋白的磷酸化水平,激活ERK信号通路,促进效应蛋白BDNF的表达,改善慢性应激大鼠的抑郁行为。

关键词: 慢性应激, 抑郁症大鼠, 推拿, ERK信号通路, BDNF

Abstract: Objective: To investigate the effects and mechanisms of massage on the depressive behavior of rats with chronic stress. Methods: The rats were subjected to chronic unpredictable mild stress for 21 days and then treated with massage for 14 days. They were divided into the following groups: blank control group, model group, massage group and fluoxetine group, with 10 rats in each group. The important acupoints of bladder meridian were massaged for 10 minutes every day (with an interval of 2 minutes, 2 times in total). Body weight, open field test, sucrose intake test and water maze test were used to evaluate the behavioral changes. The expressions of Erk/p-ERK (Extracellular signal-related kinases/Phosphorylation extracellular signal-related kinases) and BDNF in prefrontal cortex were detected by Western Blot. Results: The body weight, open field sucrose intake test and water maze data of the model group were significantly lower than those of the Control Group (P＜0.01), and the contents of p-ERK and BDNF protein in prefrontal cortex were decreased significantly (P＜0.01). The body weight, open field test, sucrose intake test and water maze test data of rats in massage group and fluoxetine group were significantly higher than those in model group(P＜0.01). The contents of p-ERK and BDNF in frontal cortex were increased significantly (P＜0.05, P＜0.01), especially in fluoxetine group (P＜0.01). Conclusion: Massage may increase the phosphorylation level of ERK protein in hippocampus and prefrontal cortex, activate ERK signaling pathway, promote the expression of BDNF, and improve the depression behavior of chronic stress rats.

Key words: chronic heat stress, depressed model rats, Massage, ERK Signal Pathway, BDNF

中图分类号:

R224

杜津莉, 李建, 孙鹏, 王宇澄, 张洁瑛. 推拿对慢性应激大鼠抑郁行为的影响及其机制[J]. 中国应用生理学杂志, 2021, 37(3): 327-331.

DU Jin-li, LI Jian, SUN Peng, WANG Yu-cheng, ZHANG Jie-ying. Effect of massage on depressive behavior of chronic stress rats and its mechanism[J]. CJAP, 2021, 37(3): 327-331.

参考文献 24

[1]	Willner P, Towell A, Samp son D, et al. Reduction of sucrose preference by chronic mild unpredictable stress and its restoration by a tricyclic antidepressant[J]. Psychopharmacology, 1987, 93(4): 358-364.
[2]	Peng ZL, Zhang C, Yan L, et al. EPA is more effective than DHA to improve depression-like behavior, glia cell dysfunction and hippcampal apoptosis signaling in a chronic stress-induced rat model of depression[J]. Int J Mol Sci, 2020, 21(5): 1-17.
[3]	Bahrami T, Rejeh N, Heravi-Karimooi M, et al. Effect of aromatherapy massage on anxiety, depression, and physiologic parameters in older patients with the acute coronary syndrome: A randomized clinical trial[J]. Int J Nurs Pract, 2017, 23(6): 1-10.
[4]	Wang Y, Jiang H, Meng H, et al. Genome-wide transcrip-tome analysis of hippocampus in rats indicated that TLR/NLR signaling pathway was involved in the pathogenisis of depressive disorder induced by chronic rest raint stress[J]. Brain Res Bull, 2017,134: 195-204.
[5]	张露芬. 实验针灸学[M]. 北京; 化学工业出版社, 2010: 221.
[6]	万小凤, 唐成林, 赵丹丹, 等. 推拿对失神经骨骼肌萎缩大鼠的治疗作用及其机制[J]. 中国应用生理学杂志, 2019, 35(3): 223-227.
[7]	郭争鸣, 王德瑜, 李迎红, 等. 三部推拿治疗抑郁症30例临床观察[J]. 中医药导报, 2014, 20(8): 100.
[8]	赵艳. 推拿手法疏理督脉和足太阳膀胱经治疗郁证体会[J]. 中国中医药信息杂志, 2008, 15(10): 78-79.
[9]	Yuan PX, Zhou RL, Wang Y, et al. Altered levels of extracellular signal-regulated kinase signaling proteins in postmortem frontal cortex of individuals with mood disorders and schizophrenia[J]. J Affect Disord, 2010, 124(1-2): 164-169.
[10]	金铭, 蔡珍珍, 刘得水. β-细辛醚改变抑郁模型大鼠行为及对海马MEK-ERK级联通路的影响[J].中国实验方剂学杂志, 2014, 2(18): 113-119.
[11]	肖楚丽, 谢一铭, 李金成, 等. 对香豆酸上调BDNF及改善慢性束缚应激诱导小鼠记忆障碍的作用[J]. 中国应用生理学杂志, 2020, 36(4): 375-378.
[12]	Teche SP, Nuemberg GL, Sordi AO, et al. Measurement methods of BDNF levels in major depression: aqualitative systematic review of clinical trials[J]. Psychiatry Q, 2013, 84(4): 485-497.
[13]	Ma H, Yu B, Kong L, et al. Neural stem cells over-expressing brain-derived neurotrophic factor(BDNF) stimulate synaptic protein expression and promote functional recovery following transplantation in rats model of traumatic brain injury[J]. Neurochem Res, 2012, 37(1): 69-83.
[14]	Mitchelmore C, Gede L. Brain derived neurotrophic factor: Epigenetic regulation in psychiatric disorders[J]. Brain Research, 2014, 1586: 162-172.
[15]	刘玥婷, 王朝阳, 王瑜, 等. 耳甲电针对抑郁症模型大鼠前额叶皮质NLRP3、NF-κB、IL-1β蛋白表达的影响[J]. 中医杂志, 2020, 61(21): 1909-1914.
[16]	綦英强. 跑步锻炼和氟西汀治疗对抑郁模型小鼠内侧前额叶皮质内毛细血管的作用及其机制研究[D]. 重庆医科大学, 2020.
[17]	潘日余. 认知重评对听障生情绪学习的调节作用及额叶激活[D]. 淮北师范大学, 2020.
[18]	高骏波. 重复经颅磁刺激治疗对青少年抑郁症自我伤害的影响作用[D]. 电子科技大学, 2020.
[19]	唐文娟, 范红艳, 张露文, 等. N-棕榈酰乙醇胺通过调控前额叶皮质PPARα通路抗大鼠抑郁样行为[J]. 中国病理生理杂志, 2020, 36(3): 451-460.
[20]	郑跃辉. 穴位推拿辅助盐酸氟西汀治疗原发性抑郁症急性期效果观察[J]. 中国乡村医药, 2016, 23(6): 38-39.
[21]	唐晓伟, 李伟, 林圣斌, 等. 刺五加对抑郁症大鼠行为学的影响[J]. 中国应用生理学杂志, 2010, 26(3): 333-335.
[22]	雷洋. 针灸推拿联合西药治疗抑郁症的临床观察[J]. 中国中医药现代远程教育, 2018, 16(11): 115-116.
[23]	柳卓, 刘检, 凌佳, 等. ELK-1/JNK/c-Fos对左归降糖解郁方(ZGJTJYF)体外培养的模拟糖尿病并发抑郁症(DD)大鼠海马神经元凋亡中的作用[J]. 中国应用生理学杂志, 2019, 35(1): 50-54.
[24]	郑盛惠, 吴玉娟, 焦建凯, 等. 围剿推拿疗法联合药物治疗围绝经期抑郁症38例疗效观察[J]. 河北中医, 2010, 32(8): 1208-1210.