海拔高度对2型糖尿病患者循环内皮祖细胞及低氧诱导因子的影响*

doi:10.12047/j.cjap.6133.2021.074

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (5): 529-533.doi: 10.12047/j.cjap.6133.2021.074

海拔高度对2型糖尿病患者循环内皮祖细胞及低氧诱导因子的影响^*

陆润兰¹, 李萍², 李兵², 邢媛², 张媛媛², 陈斌泽², 胡秦妮², 杨志华², 高保东², 哈小琴², 高彩燕^2△

1.兰州市中医医院, 兰州 730050;
2.联勤保障部队第九四〇医院, 甘肃省干细胞与基因药物重点实验室, 兰州 730050

收稿日期:2020-07-23 修回日期:2021-01-25 出版日期:2021-09-28 发布日期:2021-11-24
通讯作者: ^△Tel: 13893198703; E-mail: 506205468@qq.com
基金资助:
^*国家自然科学基金(81060015);甘肃省自然基金项目(1107RJ2A114)

Effects of altitude on circulating endothelial progenitor cells and hypoxia-inducible factor-1α in patients with type 2 diabetes

LU Run-lan¹, LI Ping², LI Bing², XING Yuan², ZHANG Yuan-yuan², CHEN Bin-ze², HU Qin-ni², YANG Zhi-hua², GAO Bao-dong², HA Xiao-qin², GAO Cai-yan^2△

1. Lanzhou Traditional Chinese Medicine Hospital, Lanzhou 730050;
2. Department of Clinical Laboratory, the 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Key Laboratory of Stem Cells and Gene Grug of Gansu Province, Lanzhou 730050, China

Received:2020-07-23 Revised:2021-01-25 Online:2021-09-28 Published:2021-11-24

摘要/Abstract

摘要： 目的: 探讨不同海拔地区2型糖尿病患者循环内皮祖细胞数量及相关检测指标的变化情况,为2型糖尿病血管并发症的研究和治疗提供依据。方法: 选取386 m低海拔地区(咸阳市)和1 520 m高海拔地区(兰州市)各一家医院内被诊断的2型糖尿病患者(25人/29人)和健康体检者(20人/20人)。用全自动生化分析仪检测两组人群的血脂、血糖和糖化血红蛋白指标,用酶联免疫吸附试验(ELISA)检测低氧诱导因子-1α(HIF-1α)的浓度,用流式细胞仪测定外周血循环内皮祖细胞(EPCs)的数量。结果: 无论在低海拔还是高海拔地区,糖尿病组较健康组循环EPCs数量降低(P＜0.01),体质指数(BMI)、腰臀比(WHR)、甘油三酯(TG)、空腹血糖(FBG)及糖化血红蛋白含量(HbAlc)增高(P＜0.05);与低海拔组相比,无论高海拔的糖尿病患者还是健康者,HIF-1α的表达水平均明显增加(P＜0.05),而循环EPCs数量明显降低(P＜0.05),且有循环EPCs数量健康者高于2型糖尿病无血管并发症者高于2型糖尿病伴血管并发症者的表现(P＜0.05)。结论: 海拔高度增加,2型糖尿病(T2DM)患者体内HIF-1α表达水平增加,循环EPCs数量降低,且与其血管病变程度密切相关。因此有望通过对高海拔地区T2DM患者进行EPCs的移植来实现对糖尿病血管并发症的预防和改善。

关键词: 2型糖尿病, 海拔, 内皮祖细胞, 低氧诱导因子-1α

Abstract: Objective: To compare the changes in the number of circulating endothelial progenitor cells and hypoxia-inducible factors in patients with type 2 diabetes at different altitudes, and to provide a basis for the research and treatment of type 2 diabetes vascular complications. Methods: Selected Type 2 diabetes patients who were diagnosed in a low altitude area of 386 m (Xianyang City) and a high altitude area of 1 520 m (Lanzhou) (25 persons/29 persons) and healthy persons (20 persons/20 persons) were selected. An automatic biochemical analyzer was used to detect the indexes of blood lipids, blood glucose, and glycosylated hemoglobin of the two groups of people, and the concentration of Hypoxia inducible factor-1α (HIF-1α) was detected by enzyme-linked immunosorbent assay (ELISA). The number of circulating endothelial progenitor cells (EPCs) in peripheral blood was determined by a cytometer. Results: No matter in low or high altitude areas, the number of circulating EPCs in the diabetes group was lower than that in the healthy group (P＜0.01). The levels of body mass index (BMI), waist to hip ratio (WHR), triglyceride (TG), fasting blood glucose (FBG) and glycosylated hemoglobin (HbAlc) were increased (P＜0.05). Compared with the low-altitude group, the expression levels of HIF-1α in diabetic patients at high-altitude and healthy people were increased significantly (P＜0.05), while the number of circulating EPCs was decreased significantly (P＜0.05), and the number of circulating EPCs in healthy people or the patients with type 2 diabetes without vascular complications was higher than that of patients with type 2 diabetes with vascular complications (P＜0.05). Conclusion: With the increase in altitude, the expression level of HIF-1α in type 2 diabetes mellitus(T2DM)patients is increased, and the number of circulating EPCs is decreased, which is closely related to the degree of vascular disease. Therefore, it is possible through transplantation of EPCs for high altitude T2DM patients to achieve the prevention and improvement of diabetic vascular complications.

Key words: type 2 diabetes mellitus, altitude, endothelial progenitor cells, hypoxia-inducible factor-1α

中图分类号:

R587.1

陆润兰, 李萍, 李兵, 邢媛, 张媛媛, 陈斌泽, 胡秦妮, 杨志华, 高保东, 哈小琴, 高彩燕. 海拔高度对2型糖尿病患者循环内皮祖细胞及低氧诱导因子的影响^*[J]. 中国应用生理学杂志, 2021, 37(5): 529-533.

LU Run-lan, LI Ping, LI Bing, XING Yuan, ZHANG Yuan-yuan, CHEN Bin-ze, HU Qin-ni, YANG Zhi-hua, GAO Bao-dong, HA Xiao-qin, GAO Cai-yan. Effects of altitude on circulating endothelial progenitor cells and hypoxia-inducible factor-1α in patients with type 2 diabetes[J]. CJAP, 2021, 37(5): 529-533.

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海拔高度对2型糖尿病患者循环内皮祖细胞及低氧诱导因子的影响^*

Effects of altitude on circulating endothelial progenitor cells and hypoxia-inducible factor-1α in patients with type 2 diabetes

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