钙敏感受体通过JNK途径参与内毒素心肌损伤*

doi:10.12047/j.cjap.6171.2021.081

中国应用生理学杂志 ›› 2021, Vol. 37 ›› Issue (5): 538-542.doi: 10.12047/j.cjap.6171.2021.081

钙敏感受体通过JNK途径参与内毒素心肌损伤^*

宋佳玉¹, 郭敬², 姜春明^1,3△

1.哈尔滨医科大学附属第一医院新生儿科, 黑龙江哈尔滨 150001;
2.阜新市第二人民医院新生儿科, 辽宁阜新 123000;
3.珠海市妇幼保健院新生儿科, 广东珠海 519060

收稿日期:2020-10-09 修回日期:2021-01-22 出版日期:2021-09-28 发布日期:2021-11-24
通讯作者: ^△Tel: 0451-85553253; E-mail: chunming0451@163.com
基金资助:
^*国家自然科学基金(81200455)

Calcium-sensing receptor promotes endotoxin-induced myocardial injury through the JNK pathway

SONG Jia-yu¹, GUO Jing², JIANG Chun-ming^1,3△

1. Department of Neonatology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001;
2. Department of Neonatology, the Second People's Hospital of Fuxin, Fuxin 123000;
3. Department of Neonatology, Zhuhai Center for Maternal and Child Health Care, Zhuhai 519060, China

Received:2020-10-09 Revised:2021-01-22 Online:2021-09-28 Published:2021-11-24

摘要/Abstract

摘要： 目的: 观察内毒素所致的心肌损伤中,钙敏感受体(CaSR)对c-Jun氨基末端激酶 (JNK)途径的影响。方法: 腹腔注射内毒素(5 mg/kg)制作新生大鼠内毒素心肌损伤模型,Wistar新生大鼠随机分为6组:对照组、内毒素组、CaSR激动剂组、CaSR抑制剂组、JNK抑制剂组、CaSR抑制剂+JNK抑制剂组。HE染色观察心肌形态, 测定血清乳酸脱氢酶(LDH)含量,PCR检测IL-6的mRNA表达,Western blot检测CaSR及JNK的蛋白表达。结果: 与对照组相比,内毒素组心肌损伤加重,LDH含量、IL-6的mRNA表达、CaSR和JNK的蛋白表达均明显增加(P＜0.05)。与内毒素组比较,CaSR激动剂组心肌损伤加重,LDH含量、IL-6的mRNA表达、CaSR和JNK的蛋白表达增加(P＜0.05); CaSR抑制剂组心肌损伤减轻,LDH含量、CaSR和JNK的蛋白表达减少(P＜0.05);JNK抑制剂组心肌损伤进一步减轻,LDH含量、IL-6的mRNA表达、CaSR和JNK的蛋白表达均减少(P＜0.05);CaSR抑制剂+JNK抑制剂组心肌损伤明显减轻,LDH含量、IL-6的mRNA表达、CaSR和JNK的蛋白表达进一步减少(P＜0.05)。结论: CaSR可能通过JNK途径参与内毒素所致的心肌损伤。

关键词: 钙敏感受体, JNK, 内毒素, 心肌损伤

Abstract: Objective: To investigate the effects of calcium-sensing receptor (CaSR) on the c-Jun N-terminal kinase (JNK) in myocardial injury induced by endotoxin. Methods: The endotoxic model of neonatal rats was made by intraperitoneal injection of LPS(5 mg/kg). Neonatal Wistar rats were randomly divided into 6 groups: ①control group (saline group), ②endotoxin (LPS) group, ③LPS + CaSR agonist group, ④LPS + CaSR inhibitor group, ⑤LPS + JNK inhibitor group, ⑥LPS + CaSR inhibitor + JNK inhibitor group. The morphology of myocardium was observed by HE staining. The content of lactate dehydrogenase(LDH) in serum was determined. And the expression of IL-6 mRNA was detected by PCR. The protein expressions of CaSR and JNK were analyzed by Western blot. Results: Compared with the control group, the myocardial injury was aggravated in the LPS group. The content of LDH and the expressions of IL-6 mRNA, CaSR and JNK were increased significantly (P＜0.05). Compared with the LPS group,myocardial injury was aggravated in the CaSR agonist group. The content of LDH and the expressions of IL-6 mRNA,CaSR and JNK were increased (P＜0.05). In the CaSR inhibitor group,myocardial injury was reduced. The content of LDH and the expressions of CaSR and JNK were decreased (P＜0.05). In the JNK inhibitor group,myocardial injury was further alleviated. The content of LDH and the expressions of IL-6 mRNA, CaSR and JNK were decreased (P＜0.05). Myocardial injury was significantly reduced in the CaSR inhibitor + JNK inhibitor group. The content of LDH and the expressions of IL-6 mRNA, CaSR and JNK were further reduced (P＜0.05). Conclusion: CaSR is involved in myocardial injury induced by LPS in neonatal rats perhaps through the JNK pathway.

Key words: calcium-sensing receptor, JNK, endotoxin, myocardial injury

中图分类号:

R363

宋佳玉, 郭敬, 姜春明. 钙敏感受体通过JNK途径参与内毒素心肌损伤^*[J]. 中国应用生理学杂志, 2021, 37(5): 538-542.

SONG Jia-yu, GUO Jing, JIANG Chun-ming. Calcium-sensing receptor promotes endotoxin-induced myocardial injury through the JNK pathway[J]. CJAP, 2021, 37(5): 538-542.

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钙敏感受体通过JNK途径参与内毒素心肌损伤^*

Calcium-sensing receptor promotes endotoxin-induced myocardial injury through the JNK pathway

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