微波辐射对肾小管上皮细胞的影响以及金雀异黄素对其保护作用

doi:10.12047/j.cjap.5382.2017.028

中国应用生理学杂志 ›› 2017, Vol. 33 ›› Issue (2): 109-111.doi: 10.12047/j.cjap.5382.2017.028

微波辐射对肾小管上皮细胞的影响以及金雀异黄素对其保护作用

马强¹, 王洪飞², 包俊强², 吴业宾², 温静¹, 齐云¹, 龚美亮³

1. 解放军总医院老年肾科, 北京 100853;
2. 解放军装甲兵学院, 安徽蚌埠 233032;
3. 解放军总医院南楼检验科, 北京 100853

收稿日期:2015-11-20 修回日期:2016-10-24 出版日期:2017-03-28 发布日期:2018-06-20
通讯作者: 马强,Tel:15810393581,E-mail:mq78448@163.com E-mail:mq78448@163.com
基金资助:
马强,Tel:15810393581,E-mail:mq78448@163.com

Protective effects of Genistein on human renal tubular epithelial cells damage of microwave radiation

MA Qiang¹, WANG Hong-fei², BAO Jun-qiang², WU Ye-bin², WEN Jing¹, QI Yun¹, GONG Mei-liang³

1. Department of Geriatric Nephrology, PLA General Hospital, Beijing 100853;
2. College of Armored Forces, Bengbu 233010;
3. Department of Clinical Laboratory, General Hospital of PLA, Beijing 100853, China

Received:2015-11-20 Revised:2016-10-24 Online:2017-03-28 Published:2018-06-20
Contact: 马强,Tel:15810393581,E-mail:mq78448@163.com E-mail:mq78448@163.com
Supported by:
马强,Tel:15810393581,E-mail:mq78448@163.com

摘要/Abstract

摘要： 目的：观察微波辐照对人近端肾小管上皮细胞（HKC）的影响及金雀异黄素对其的保护作用。方法：HKC分为对照组、微波辐照组、金雀异黄素组（n=6）。金雀异黄素组在辐照前2 h用含30μmol/L金雀异黄素的DMEM培养基进行预培养。辐照后24 h留取上清进行乳酸脱氢酶（LDH）、β-N-乙酰氨基葡萄糖苷酶（NAG）活性及丙二醛（MDA）、超氧化物歧化酶（SOD）活性检测。Hoechst 33258染色观察细胞凋亡情况。结果：与对照组比较，微波辐照组上清NAG、LDH活性明显增加（P < 0.01），金雀异黄素预处理组则较微波辐照组明显下降（P < 0.01）；微波辐照组上清活性也较对照组明显增加（P < 0.01）。Hoechst 33258染色显示，微波辐照可导致较多量的细胞凋亡，而应用金雀异黄素预处理后细胞凋亡的比例均大大减少。微波辐照可大大提高HKC细胞中的MDA含量，SOD活性降低（P< 0.01），应用金雀异黄素预处理后MDA的含量无明显降低，SOD的活性明显增大（P < 0.01）。结论：微波辐照可导致人近端肾小管上皮细胞出现功能损伤，金雀异黄素对其具有一定的保护作用，可能与其减轻氧化应激损伤、减少细胞凋亡有关。

关键词: 金雀异黄素, 肾小管上皮细胞, 电磁辐射

Abstract: Objective: To observe the effects of microwave irradiation on human proximal renal tubular epithelial cells (HKC) and protec-tive effects of genistein. Methods: HKC cells were divided into control group, microwave irradiation group and genistein group(n=6) re-spectively. The genistein group cells were pre-incubated with 30μmol/L genistein in DMEM for 2 hours. After irradiation for 24 hours, the concentrations of lactate dehydrogenase(LDH) and β-N-acetyl glucosaminidase(NAG) in culture solution were measured to evaluate cell injury. Cells were curetted to measure the levels of malondialdehyde (MDA) and superoxide dismutase (SOD). Cell apoptosis and necrosis were de-tected with Hoechst 33258 stain. Results: Compared with the control group, the NAG activity of the microwave irradiation group was signifi-cantly increased(P < 0.01), and NAG activity of genistein pre-incubated group was significantly decreased(P < 0.01). The levels of LDH in microwave irradiation group were also increased significantly (P < 0.01 vs control group). LDH levels could be decreased obviously (P < 0.01 vs microwave irradiation group) after genistein pre-incubate. Hoechst 33258 fluorescent staining revealed that the nucleus crimpled, cres-cent liked and chromatin condensed, even nucleus disintegrated. Our research showed that microwave irradiation could lead to large amount of cell apoptosis and necrosis, and genistein pre-treatment could reduce the ratio of apoptosis and necrosis than that in microwave irradiation group (P < 0.01). The concentration of MDA in microwave irradiation group was higher than that in control group (P < 0.01). At the same time, the activity of SOD was significantly reduced (P < 0.01). Pre-incubated with genistein could not decrease the MDA levels, but could increase the activities of SOD (P < 0.01 vs microwave irradiation group). Conclusion: microwave irradiation can induce human proximal renal tubular epithelial cells injury. The protective effects of genistein may partly correlated with decreasing oxidative stress damage and cell apoptosis in HKC cells.

Key words: Genistein, renal tubular epithelial cell, microwave radiation

马强, 王洪飞, 包俊强, 吴业宾, 温静, 齐云, 龚美亮. 微波辐射对肾小管上皮细胞的影响以及金雀异黄素对其保护作用[J]. 中国应用生理学杂志, 2017, 33(2): 109-111.

MA Qiang, WANG Hong-fei, BAO Jun-qiang, WU Ye-bin, WEN Jing, QI Yun, GONG Mei-liang. Protective effects of Genistein on human renal tubular epithelial cells damage of microwave radiation[J]. CJAP, 2017, 33(2): 109-111.

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微波辐射对肾小管上皮细胞的影响以及金雀异黄素对其保护作用

Protective effects of Genistein on human renal tubular epithelial cells damage of microwave radiation

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