不同浓度酒精任氏液对蛙坐骨神经干双相复合动作电位的影响*

doi:10.12047/j.cjap.5750.2019.050

中国应用生理学杂志 ›› 2019, Vol. 35 ›› Issue (3): 232-238.doi: 10.12047/j.cjap.5750.2019.050

不同浓度酒精任氏液对蛙坐骨神经干双相复合动作电位的影响^*

黄志华¹⁺, 魏培坚¹⁺, 江玲²⁺, 陈穗²⁺, 程必红¹, 林颖¹, 吴林耿¹, 许秋雄¹, 吴少伟¹, 王海燕^3△, 沈建新^{2, 4△}

1. 汕头大学医学院临床医学专业本科生,
2. 汕头大学医学院机能学实验室,
3. 汕头大学医学院病理生理学教研室,
4. 汕头大学医学院生理学教研室,广东汕头 515041

出版日期:2019-05-28 发布日期:2019-06-28
通讯作者: Tel: 13612311390; E-mail: jxshen@stu.edu.cn.
⁺: 为共同第一作者
基金资助:
2017年“攀登计划”广东大学生科技创新培育专项资金资助项目(pdjh2017a0196);汕头大学医学院省级大学生创新训练项目(201610560108);汕头大学医学院省级机能学实验教学示范中心建设项目(2015-2018);广东省2016年扬帆计划资金-培养高层次人才项目

Effects of Ringer’s solution with different concentrations of alcohol on biphasic compound action potentials of frog sciatic nerve trunk

HUANG Zhi-hua¹⁺, WEI Pei-jian¹⁺, JIANG Ling²⁺, CHEN Sui²⁺, CHENG Bi-hong¹, LIN Ying¹, WU Lin-geng¹, XU Qiu-xiong¹, WU Shao-wei¹, WANG Hai-yan^3△, SHEN Jian-xin^2,4△

1. Department of Clinical Medicine (Undergraduate Student), Shantou University Medical College, Shantou 515041;
2. Lab of Medical Functional Sciences, Shantou University Medical College, Shantou 515041;
3. Department of Pathophysiology, Shantou University Medical College, Shantou 515041;
4. Department of Physiology, Shantou University Medical College, Shantou 515041, China

Online:2019-05-28 Published:2019-06-28

摘要/Abstract

摘要： 目的:定量探究1%～80%酒精任氏液对蛙坐骨神经干双相复合动作电位(AP)的作用及其恢复情况。方法:制备长为6～8 cm的蛙坐骨神经干标本,分别将含有0%、1%、2%、4%、8%、16%、32%、48%、64%、80%酒精的标准任氏液通过新加装在神经屏蔽盒中的加药液槽浸泡位于刺激电极和接地电极之间的神经干各5 min,用BL-420F系统分别记录由刺激所诱发的双相AP;之后用标准任氏液冲洗5遍,浸泡5 min, 分别记录冲洗后AP。结果:与标准任氏液相比, ≤4%酒精任氏液对AP峰值和传导速度无影响,≥8%酒精任氏液作用后可使AP峰值和传导速度均下降;16%、32%和≥48%酒精任氏液作用后分别使神经干失去产生AP能力的比例为30%、90%和100%。≤32%酒精任氏液作用后冲洗,AP峰值可完全恢复;48%、64%和80%酒精任氏液作用后冲洗,能恢复产生动作电位能力的比例分别为90%、40%和0%,平均峰值分别下降至正常的60%、36%和0%;冲洗后: ≤8%酒精任氏液作用后的AP速度与正常无异,≥16%酒精任氏液作用后的AP速度无法完全恢复。结论:不同浓度酒精对AP峰值和传导速度影响不同,这对酒精的合理使用及过度使用后的损伤恢复有参考意义。

关键词: 不同浓度酒精任氏液, 牛蛙, 坐骨神经干, 双相动作电位

Abstract: Objective: To quantitatively investigate the effects of Ringer’s solution with different concentrations of alcohol (1%～80%) on biphasic compound action potentials (AP) from frog sciatic nerve trunk, and their recoveries from alcohol effects. Methods: Individual segments of frog sciatic nerve trunk with a length of 6 to 8 cm were prepared. Ringer’s solution with different concentrations of alcohol (0%, 1%, 2%, 4%, 8%, 16%, 32%, 48%, 64% and 80%) was applied onto the segment of the trunk between the stimulus and ground electrodes via an agent reservoir which was newly armed in a nerve trunk shielded chamber for 5 minutes. The nerve trunk was respectively electro-stimulated to generate the biphasic compound AP which was recorded using the experimental system of BL-420F. This was followed by 5 times washout plus 5 min administration with Ringer’s solution before recovery recording of AP. Results: Compared to normal Ringer’s solution, Ringer’s solution with alcohol at ≤4% did not have dramatic impacts on the AP amplitude and conduction velocity, while Ringer’s solution with alcohol at ≥8% there was significant decrease in these two parameters. Ringer’s solution with alcohol at the conentrations of 16%, 32% and ≥48% could prevent a small proportion (30%), a large proportion (90%) and all (100%) of sciatic nerve trunks, respectively, from generating AP. Washout with normal Ringer’s solution after alcohol application at the concentration of ≤32%, AP could totally recover to normal status. While alcohol at the concentration of 48%, 64% and 80%, the probabilities to regenerate APs were 90%, 40% and 0%, and the AP amplitudes were decreased to 60%, 36% and 0%, respectively. After washout, AP conduction velocity showed no difference with alcohol at the concentration of ≤8% when compared with that before washout, while it could not be recovered to normal under alcohol at ≥16%. Conclusion: Ringer’s solution with different concentrations of alcohol exerts different effects on biphasic compound AP amplitude and conduction velocity. Hopefully, our findings could be helpful for the alcoholic usage and its recovery from alcoholic damage.

Key words: Ringer’s solution with different concentration of alcohol, bullfrog, sciatic nerve trunk, biphasic compound action potentials

黄志华, 魏培坚, 江玲, 陈穗, 程必红, 林颖, 吴林耿, 许秋雄, 吴少伟, 王海燕, 沈建新. 不同浓度酒精任氏液对蛙坐骨神经干双相复合动作电位的影响^*[J]. 中国应用生理学杂志, 2019, 35(3): 232-238.

HUANG Zhi-hua, WEI Pei-jian, JIANG Ling, CHEN Sui, CHENG Bi-hong, LIN Ying, WU Lin-geng, XU Qiu-xiong, WU Shao-wei, WANG Hai-yan, SHEN Jian-xin. Effects of Ringer’s solution with different concentrations of alcohol on biphasic compound action potentials of frog sciatic nerve trunk[J]. CJAP, 2019, 35(3): 232-238.

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不同浓度酒精任氏液对蛙坐骨神经干双相复合动作电位的影响^*

Effects of Ringer’s solution with different concentrations of alcohol on biphasic compound action potentials of frog sciatic nerve trunk

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