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CJAP ›› 2017, Vol. 33 ›› Issue (3): 222-225.doi: 10.12047/j.cjap.5384.2017.055

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Effects of caspase inhibitor z-VAD-fmk on the expressions of calumenin, caspase-3, GRP78 and GRP94 in adriamycin-injured cardiomyocytes

BAO Yun-long1, Wang Yu2, WU Zhe1, CUI Xiao-xue2, DU Jian-jun1, WANG Yi-lin2, ZHAO Ming2   

  1. 1. The Inner-Mongolia Tongliao City Hospital, Tongliao 028000, China;
    2. The Inner-Mongolia National University, Tongliao 028000, China
  • Received:2015-11-24 Revised:2016-11-22 Online:2017-05-28 Published:2018-06-20
  • Supported by:
    内蒙古自然科学基金项目(2015MS08150)

Abstract: Objective: To study the effects of Caspase broad spectrum inhibitor Z-VAD-FMK on the expressions of calumenin,caspase-3, GRP78 and GRP94 in adriamycin-injured cardiomyocytes and to discuss whether there is a regulation relationship between calumenin and endo-plasmic reticulum stress and myocardial apoptosis. Methods: The primary cultured suckling mouse myocardium were randomly divided into control group (cardiomyocyte), adriamycin group (3 mg/L adriamycin + cardiomyocyte) and z-VAD-fm group (3 mg/L adriamycin + 0.1 μmol/L Z-VAD-fmk + cardiomyocyte), each group of cardiomyocytes were cultured in CO2 incubator at 37℃ for 24 h (n=3). The expres-sion ofα -SMA protein in ventricular myocytes was detected by immunohistochemical method. The expressions of calumenin, GRP78, GRP94 and Caspase-3 in the myocardial cells were detected by Western blot. Results: Compared with the control group, the expression of calumenin in adriamycin induced myocardial cells was significantly decreased (P < 0.01), while the expressions of GRP78, GRP94 and Caspase-3 ex-pression were increased (P < 0.01). Compared with adriamycin group, the expression of calumenin in z-VAD-fm group was increased (P < 0.01), while the expressions of GRP78, GRP94 and caspase-3 were decreased (P < 0.01). Conclusion: The caspase inhibitor z-VAD-fmk inhibited the expression of caspase and increased the expression of calumenin in adriamycin induced myocardial cells, and thus alleviated the endoplasmic reticulum stress.

Key words: adriamycin, endoplasmic reticulum stress, calumenin, suckling mouse, z-VAD-fmk

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