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CJAP ›› 2017, Vol. 33 ›› Issue (2): 105-108.doi: 10.12047/j.cjap.5407.2017.027

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Effects of oxygen-glucose deprivation on the permeability of human umbilical vein endothelial cells

HAN Xue1, HUANG Xin2, ZHU Ling-ling2, FAN Ming1,2   

  1. 1. Department of Neurobiology, Capital Medical University, Beijing 100069;
    2. Department of Cognitive Science, Beijing Institute of Basic Medical Science, Beijing 100850, China
  • Received:2016-03-29 Revised:2016-11-25 Online:2017-03-28 Published:2018-06-20
  • Contact: 范明,Tel:010-66931315,E-mail:fanmingchina@126.com;朱玲玲,E-mail:linglingzhu@hotmail.com E-mail:fanmingchina@126.com;linglingzhu@hotmail.com
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Abstract: Objective: To investigate the effects of oxygen-glucose deprivation (OGD) on the functions of blood-brain barrier in vitro, we established an OGD model to mimic cerebral ischemic injury in human umbilical vein endothelial cells (HUVECs). Methods: Cells were cul-tured in DMEM without glucose under hypoxic conditions (0.3% O2) for different time point. The survival rate of HUVECs was detected by CCK-8 assay. The permeability of HUVECs was measured by epithelial volt-ohm meter. The expression of tight junction protein was detected by Western blot analysis. Results: In OGD group, the survival rate of HUVECs was obviously decreased. The permeability of HUVECs also decreased in OGD-treated cells. And the expression of tight junction protein was reduced after OGD treatment. Conclusion: Our data indicate that OGD treatment disrupt the barrier function of tight junctions and increase permeability of HUVECs and finally accelerated cell death.

Key words: oxygen-glucose deprivation, tight junction, permeability, HUVEC

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