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CJAP ›› 2017, Vol. 33 ›› Issue (3): 204-211.doi: 10.12047/j.cjap.5435.2017.051

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Dynamic changes of ‘substantianigra-ventralislateralis-cortex’ pathway neural activity coherence and neurotransmitters in rat during exhausting exercise

HU Yan-ru1,2, LIU Xiao-li1, QIAO De-cai1   

  1. 1. College of Physical Education and Sports, Beijing Normal University, Beijing 100875;
    2. Department of Physical Education, Central South University, Changsha 410083, China
  • Received:2016-03-07 Revised:2016-03-07 Online:2017-05-28 Published:2018-06-20
  • Supported by:
    国家自然科学基金资助项目(31171138,31571221);湖南省体育科学学会重点资助项目(2015HN016)

Abstract: Objective: To reveal the possible mechanism of changes of ‘substantianigra-ventralislateralis-cortex’ pathway neural activity during one bout of exhausting exercise through observing the neural activity coherence between different nucleus and the concentration of extra-cellular glutamate (Glu) and gamma-aminobutyric acid (GABA). Methods: Male Wistar rats were randomly divided into neural activity real-time observation group, substantianigra (SNr) extracellular neurotransmitters observation group, ventralislateralis (VL) extracellular neuro-transmitters observation group and supplementary motor area (SMA) extracellular neurotransmitters observation group, 10 rats in each group. For rats of neural activity real-time observation group, by using LFPs and ECoG recording technique, and self-comparison, we simultaneously recorded the dynamic changes of neural activity of rat SNr, VL and SMA during one bout of exhausting exercise. The dynamic changes of ex-tracellular Glu and GABA in rat SNr, VL and SMA were also observed through microdialysis combined high performance liquid chromatography (HPLC) technique and self-comparison method. Results: Based on the behavioral performance, the exhausting exercise process could be di-vided into 5 different stages, the rest condition, auto exercise period, early fatigue period, exhaustion condition and recovery period. The elec-trophysiological study results showed that, the coherence between neural activity in rat SNr, VL and SMA was significant between 0~30 Hz during all the procedure of exhausting exercise. Compared with the rest condition, the microdialysis study showed that the Glu concentrations and Glu/GABA ratio in SNr were decreased significantly during automatic exercise period (P < 0.05, P < 0.01), the GABA concentrations were increased significantly (P < 0.05, P < 0.01), while, in VL and cortex, the Glu concentrations and Glu/GABA ratio were increased significantly (P < 0.05, P < 0.01), the GABA concentrations were decreased significantly (P < 0.05, P < 0.01). Under early fatigue and ex-haustion conditions, compared with the rest condition,the Glu concentrations and Glu/GABA ratio in SNr were increased significantly (P < 0.05, P < 0.01), the GABA concentrations were decreased significantly (P < 0.05, P < 0.01), while the Glu concentrations and Glu/GABA ratio in VL and cortex were decreased significantly (P < 0.05, P < 0.01), the GABA concentrations were increased significantly (P < 0.05, P < 0.01). Conclusion: The neural net work communication between ‘substantianigra-ventralislateralis-cortex’ pathway exists, changes of Glu and GABA in the nucelus of the pathway are one of the factors resulting in the changes of neural activity.

Key words: exhausting exercise, rat, ‘substantianigra-ventralislateralis-cortex&rsquo, pathway, coherence, neurotransmitter

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