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CJAP ›› 2017, Vol. 33 ›› Issue (3): 218-221.doi: 10.12047/j.cjap.5442.2017.054

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Effects of PKCα/c-fos, Bax/Bcl-2 expression on the proliferation and apoptosis of rat pulmonary arterial smooth muscle cells in hypoxia

CAO Yan-hong1, PAN Jian-xun1, LIANG Yue-qin2, SHI Rui-zan2   

  1. 1. Datong the Fifth People's Hospital, Datong 037000;
    2. Shanxi Medical University, Taiyuan 030001, China
  • Received:2016-03-28 Revised:2016-12-07 Online:2017-05-28 Published:2018-06-20
  • Supported by:
    山西省青年科技研究基金资助项目(2014021038-3);山西医科大学校科技创新基金资助项目(01201308);山西医科大学博士启动基金资助项目(B032001207)

Abstract: Objective: To explore the effects of PKCα/c-fos, Bax/Bcl-2 on the proliferation and apoptosis of rat pulmonary arterial smooth musclecells(PASMCs) in hypoxia. Methods: The PASMCs of rats had been isolated and cultured, and then were cultured under normoxia (5%CO2、21%O2、74%N2)and hypoxia(5%CO2,2% O2,93%N2)condition for 24 h and 48 h, respectively. The proliferation of PASMCs was tested by methylthiazolyltetrazolium (MTT). The changes of PASMCs apoptosis were detected by Annexin V-FITC/PI staining combined flow cytometry. RT-PCR and Western blot analysis were performed to detect the gene and protein levels of PKCα/c-fos, Bax/Bcl-2, respectively. All experiments were repeated three times with at least triplicate samples. Results: The proliferation of PASMCs in hypoxia group were stronger than that of normoxia group after 24 h and 48 h (P < 0.01), while the apoptosis rate did not change significantly. Meanwhile, the higher levels of PKCα, c-fos, Bcl-2 mRNA and proteins after hypoxia for 24 h and 48 h were detected by RT-PCR and Western blot analysis, while the ex-pression levels of Bax had no significant difference under normoxic and hypoxic conditions. Conclusion: Elevated proliferation and expression of PKCα, c-fos, Bcl-2 was observed in rat PASMCs in hypoxia, and while the apoptosis rate had no significant change.

Key words: PKCα/c-fos, Bax/Bcl-2, proliferation, apoptosis, hypoxia

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