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CJAP ›› 2017, Vol. 33 ›› Issue (1): 78-82.doi: 10.12047/j.cjap.5448.2017.020

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Effect of long noncoding RNA SPRY4-IT1 on proliferation and metastasis of medulloblastoma

SHI Peng-fei1, JI Hai-long2, LUO Yong-kang2, MAO Tian-ming2, CHEN Xi3, ZHOU Kai-yu1   

  1. 1. the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000;
    2. Department of Neurosurgery, Zhejiang Taizhou Municipal Hospital, Taizhou 318000, China;
    3. Department of Pathology, Zhejiang Taizhou Municipal Hospital, Taizhou 318000, China
  • Received:2016-03-28 Revised:2016-10-24 Online:2017-01-28 Published:2018-06-19
  • Supported by:
    浙江省中医药管理局科技计划资助项目(2013ZA133,2015ZB133);台州市科技局科技计划资助项目(14SF05)

Abstract: Objective: To investigate the effects of long non-coding RNA SPRY4-IT1 (LncRNA) on proliferation and metastasis of medul-loblastoma cells.Methods: SPRY4-IT1siRNA and control fluorescence siRNA were transfected into medulloblastoma cell line Daoy with Lipo-fe ctamine 2000 and were divided into control group and si-SPRY4-IT1 group. Relative expression of SPRY4-IT1 mRNA were detected by real-time quantitative PCR. The change of cell proliferation were examined using CCK-8 kit and clone forming experiment. The change of cell inva-sion and metastasis were examined by matrigel invasion assay and cell metastasis experiment respectively, The expression of matrix metallopro-teinase MMP-2 and MMP-9 were detected by Western blot.Results: In si-SPRY4-IT1 group,the SPRY4-IT1 mRNA expression level, cell pro-liferation in vitro,cell invasion and migration ability, MMP-2 protein expression were significantly lower than those in the control group.Conclusion: Interference SPRY4-IT1 expression has prominent inhibitory effect on the cell proliferation、invasion and metastasis of medulloblastoma cell line Daoy.

Key words: medulloblastoma, long noncoding RNA (LncRNA), SPRY4-IT1, proliferation, invasion and metastasis

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