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CJAP ›› 2017, Vol. 33 ›› Issue (5): 471-475.doi: 10.12047/j.cjap.5494.2017.113

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Effects of TAK gene silencing on the expressions of IL-6 and IL-8 induced by TNF-α in fibroblast-like synoviocytes

MO Xuan-rong1, XIE Jiang-wen2, LV Guo-ju2, KE Yu-ping1, LUO Xin-jing1   

  1. 1. Department of Basic Medical Sciences, School of Medicine of Taizhou University, Taizhou 318000;
    2. Department of Cardiology, Yingzhou District Second people's Hospital, Ningbo 315000, China
  • Received:2016-09-09 Revised:2017-05-15 Online:2017-09-28 Published:2018-06-19
  • Supported by:
    国家自然科学基金面上项目(81373139);浙江省自然科学基金(LY16H100001);浙江省医药卫生科研项目(2015KYA206)

Abstract: Objective: To investigate the effects of silencing transforming growth factor-β activating kinase 1 (TAK1)on the expressions of IL-6 and IL-8 induced by TNF-α in fibroblast-like synoviocytes, and to explore the role of TAK1 in rheumatoid arthritis (RA).Methods: The synthesized TAK1 siRNA and scrambled siRNA (ScRNA) were transferred into cultured RA fibroblast-like synoviocyte line MH7A by lipofectamine. The expressions of the pro-inflammatory mediator IL-6 and IL-8 and the levels of phospho-P38(p-P38), phospho-C-Jun NH2-terminal kinase(p-JNK), phospho-extracellular signal-regulated kinase(p-ERK), phospho-p65(p-p65) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha(IκBa) were examined.Results: Silencing of TAK was demonstrated in synoviocytes transfected by TAK siRNA. TAK1 silencing markedly attenuated the expression of IL-6 and IL-8 in the presence of TNF-α. TAK1 silencing inhibited the activation of p38 and JNK MAPK. TAK1 silencing also inhibited activation of nuclear factor-κB (NF-κB).Conclusion: TAK1 silencing attenuated the expression of IL-6 and IL-8 in synoviocytes induced by TNF-α via inhibiting the activation of p38, JNK MAPK and NF-κB.

Key words: rheumatoid arthritis, synoviocytes, inflammatory mediators, TAK1

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