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CJAP ›› 2017, Vol. 33 ›› Issue (3): 267-270.doi: 10.12047/j.cjap.5514.2017.065

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Effect of high concentration glucose on the expression of ALDH2 in cardiac fibroblasts of rats

GU Xiao-yu1,3, FANG Ting-ting3, GAO Qin3, KANG Pin-fang2, LI Zheng-hong3, CHENG Xiang-yang1   

  1. 1. Department of Anesthesiology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
    2. Department of Cardiovascular Medicine, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004;
    3. Department of Physiology, Bengbu Medical College, Bengbu 233030, China
  • Received:2016-09-13 Revised:2017-01-25 Online:2017-05-28 Published:2018-06-20
  • Supported by:
    国家自然科学基金资助项目(81550036);安徽省自然科学基金资助项目(1508085MH169,1508085MH170);蚌埠医学院研究生科研创新计划项目(Byycx1548)

Abstract: Objective: To observe whether acetaldehyde dehydrogenase 2 (ALDH2) is expressed in cardiac fibroblasts and investigate the change of ALDH2 in cardiac fibroblasts when cultured with high concentration of glucose. Methods: Cultured cardiac fibroblasts were randomly divided into four groups:normal control group (5.5 mmol/L glucose), Alda-1 (the agonist of ALDH2, 20μmol/L) group, high glucose group (30 mmol/L glucose) and high glucose + Alda-1 group. Cardiac fibroblasts were identified by immunofluore-scence technique. Cell prolifera-tion was detected by MTT method after treated with drugs for 48 hours. mRNA and protein expressions of ALDH2 were determined by RT-PCR and Western blot, aimed to ensure whether ALDH2 was expressed in cardiac fibroblasts. The changes of ALDH2 protein expression in cardiac fibroblasts were tested by Western blot. Results: RT-PCR and Western blot results revealed that ALDH2 was expressed in cardiac fibroblasts. Compared with normal control group, cardiac fibroblasts proliferation was increased (P < 0.05), while the protein expression of ALDH2 was reduced (P < 0.05) in high glucose group. When treated with Alda-1, the proliferation of cardiac fibroblasts was decreased (P < 0.01), while the protein expression of ALDH2 was increased (P < 0.05) in high glucose group. Conclusion: ALDH2 was expressed in cardiac fi-broblasts. Alda-1, the agonist of ALDH2 enhanced the expression of ALDH2 and inhibited the proliferation of cardiac fibroblasts when cultured with high concentration of glucose.

Key words: cardiac fibroblasts, acetaldehyde dehydrogenase 2, high concentration of glucose, Alda-1, rat

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