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CJAP ›› 2018, Vol. 34 ›› Issue (3): 263-267.doi: 10.12047/j.cjap.5540.2018.062

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Effects of purslane polysaccharide on the intestinal tissue IL-6/STAT3 and NF-kappa B of ulcerative colitis

FAN Wen-tao, WANG Pan-hong, WANG Qian   

  1. Shanxi University of Chinese Medicine, Xianyang 712046, China
  • Received:2017-06-15 Revised:2017-12-27 Online:2018-05-28 Published:2018-09-08
  • Supported by:
    国家自然科学基金(81202847,81470188,81673832);陕西省科技厅社发攻关项目(2016SF-375);陕西省中医药管理局项目(13-LC073)

Abstract: Objective: 2minus 2-trinitrobenzene sulfonic acid (TNBS) copy ulcerative colitis model of rats, to explore purslane polysaccharide on ulcerative colitis rats colon organization IL6/STAT3 and the influence of the NF-kappa B, clear IL-6/STAT3 signaling pathway and the relationship of chronic inflammatory bowel disease, looking for new targets for the treatment of chronic ulcerative colitis.Methods: Fourty rats were randomly divided into control group, model group, mesalazine group, and purslane group (n=10). The colonic contents were weighed and weighed again after drying, and the colonic tissues were biopsies. The contents of serum interleukin-6 (IL-6), interleukin 1 β (IL-1 β), tumor necrosis factor α (TNF-α), nuclear transcription factor kappa B (NF-kappa B) were detected by ELISA kit. Immunohistochemical staining was used to determine the colonic MPO (myeloperoxidase). RT-PCR method was used to detect signal transduction and transcriptional activator 3 (STAT3), IL-6 mRNA.Results: Compared with model group and mesalazine group, defecation status of rats in purslane group were markedly improved, intestinal mucosa edema reduced, the serum IL-6, intestinal tissue MPO, the NF-kappa B and serum sIL-6Rα, gp130 content were decreased (P<0.01). Compared with the model group, the expression levels of STAT3 and IL-6 mRNA in the purslane group were significantly reduced (P<0.01). There was no significant difference of testing indicator mentioned above between control group and purslane group (P>0.05).Conclusion: By lowering the serum IL-6, sIL-6Rα and gp130 in rats, the purslane polysaccharides reduced the levels of MPO and NF-kappa B in the intestinal tissues, and further reduced the inflammatory response caused by the formation of sIL-6R alpha and IL-6. By using IL-6/STAT3 signaling pathway, the mRNA expression levels of STAT3 and IL-6 of rats intestinal tissues were down-regulated to inhibit the occurrence of inflammation.

Key words: Purslane polysaccharide, inflammatory bowel disease, IL-6/STAT3 signaling pathway, rat

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