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CJAP ›› 2018, Vol. 34 ›› Issue (3): 197-200.doi: 10.12047/j.cjap.5579.2018.047

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Effect of treating with anti-CCL21 monoclonal antibody on left ventricular remodeling and cardiac function post-acute myocardial infarction in mice

JIANG Yi, YAN Yan-li, BAI Jian-wen   

  1. Department of Internal Medicine, Emergency Center, East Hospital, Tongji University School of Medicine, Shanghai 200120, China
  • Received:2017-03-07 Revised:2018-03-05 Online:2018-05-28 Published:2018-09-08
  • Contact: 白建文,Tel:021-38804518;E-mail:bjwhos@163.com E-mail:bjwhos@163.com
  • Supported by:
    白建文,Tel:021-38804518;E-mail:bjwhos@163.com

Abstract: Objective: To explore the therapeutic effect of treating with anti-CCL21 monoclonal antibody on left ventricular remodeling and cardiac function post-acute myocardial infarction in mice.Methods: C57BL/6 mice were randomly divided into sham-operated group, model group and anti-CCL21 monoclonal antibody intervention group, and further randomly divided into 1 d, 3 d,7 d and 21 d subgroups. A mouse model of acute myocardial infarction (AMI) was generated by left anterior descending coronary artery ligation in C57BL/6 mice. Mice in model group were intravenously given isotype-IgG 1.0 mg, mice in anti-CCL21 monoclonal antibody intervention group were intravenously given goat anti-mouse CCL21 monoclonal antibody 1.0 mg at 5 minute and on the 3rd day post-coronary artery ligation. After surgery, C-C motif chemokine receptor 7 (CCR7) expression in myocardium was detected on the 1st, 3rd, 7th day, and the expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 in myocardium were detected on the 7th day 8 mice per group using Western blot. Serum levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in each group were measured on the 1st, 3rd, 7th day post-surgery using ELISA, 8 mice per group. Transthoracic echocardiography was underwent in mice on the 7th and 21th day after surgery using an echocardiography system to evaluate left ventricular function.Results: Compared with sham-operated group, serum levels of CCL21, TNF-α, IL-6, and myocardiac expression of CCR7、MMP-2、MMP-9 in model group were significantly increased (P<0.05). Compared with model group, serum levels of TNF-α and IL-6 and myocardiac expression level of MMP-9 were significantly decreased in anti-CCL21 monoclonal antibody intervention group(P<0.05).Conclusion: Our study indicates that treating with anti-CCL21 monoclonal antibody is involved in preventing cardiac remodeling and protecting left ventricular function post AMI via inhibiting inflammatory response and MMP-9 expression level.

Key words: myocardial infarction, inflammatory response, MMP-9, cardiac remodeling, CCL21, mice

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