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CJAP ›› 2018, Vol. 34 ›› Issue (5): 396-400.doi: 10.12047/j.cjap.5621.2018.090

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Placenta-derived mesenchymal stem cells with HLA-G positive expression induce Treg in vitro

CUI Gui-yu1, BAI Jian2, MIAO Lan-ying2, LIN Da-yong2, LIU Hong2, LI Ya-li3,4, LIU Xi-cheng3,4   

  1. 1. Medical School of Inner Mongolia University for Nationalities, Tongliao 028000;
    2. Liao Ning University of Traditional Chinese Medicine, Shenyang 110847;
    3. Department of Anesthesiology, Shenzhen People's Hospital, Shenzhen 518020;
    4. Shenzhen Anesthesiology Engineering Center, Shenzhen 518020, China
  • Received:2017-07-27 Revised:2018-02-11 Online:2018-09-28 Published:2019-02-21
  • Supported by:
    国家自然科学基金面上项目(81571555);辽宁省自然科学基金(2015020714)

Abstract: Objective: To study placenta-derived mesenchymal stem cells with HLA-G (Human Leukocyte Antigen, HLA-G) positive expression induce Treg (regulatory T cell, Treg) in vitro. Methods: placenta-derived mesenchymal stem cells were separated from neonatal placenta; PEGFP - N1 -HLA-G plasmid was transfected in placenta-derived mesenchymal stem cells by liposome transfection.The cells were divided into 3 groups including control group, PEGFP-N1 group and PEGFP-N1-HLA-G group, 5 complex walls in each group. Expression of HLA-G protein was detected by Western Blotting; after identification of cells, healthy human peripheral blood CD4+ T lymphocytes were cultured with placenta-derived mesenchymal stem cells with HLA-G positive expression, and the ratio of CD4+CD25+Foxp3+Treg in T lymphocytes was accounted. Results: After transfection of PEGFP-N1-HLA-G, the placenta-derived mesenchymal stem cells can express HLA-G protein significantly, compared with the control group and PEGFP - N1 group (P<0.01). After HLA-G positive placenta-derived mesenchymal stem cells and CD4 + T lymphocytes were cultured for 24 h, the ratio of CD4+CD25+Foxp3+Treg in T lymphocytes was (16.41±0.94)%. After HLA - G positive placenta-derived mesenchymal stem cells and CD4+ T lymphocytes were cultured for 48 h, the ratio of CD4+CD25+Foxp3+Treg in T lymphocytes was (16.46±0.59)% significantly, compared with the control group and PEGFP - N1 group (P<0.01). Conclusion: Placenta-derived mesenchymal stem cells modified by HLA-G gene can effectively induce CD4+CD25+Foxp3+Treg in vitro.

Key words: human leucocyte antigen-G, placenta-derived mesenchymal stem cell, T lymphocyte, regulatory T lymphocyte

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