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CJAP ›› 2018, Vol. 34 ›› Issue (5): 404-407.doi: 10.12047/j.cjap.5624.2018.092

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Effects of endothelial progenitor cells on proliferation and biological function of hepatic stellate cells under shear stress

CHEN Xin-xin1, ZHANG Xiao-yun2, DING Yu-zhen1, LI Xin2, GUAN Xiu-mei2, LI Hong2, CHENG Min2, CUI Xiao-dong2   

  1. 1. College of Biological Science and Technology, Weifang Medical University, Weifang 261053, China;
    2. Clinical Medical College, Weifang Medical University, Weifang 261053, China
  • Received:2017-08-28 Revised:2018-02-02 Online:2018-09-28 Published:2019-02-21
  • Supported by:
    国家自然科学基金(31570941,81700406,31270993);山东省自然科学基金(ZR2014JL018,ZR2016CM20);山东省高等学校科技计划项目(J15LK08,J14LK59,J14LK12);国家大学生创新计划项目(201510438013);潍坊医学院大学生科技创新基金(KX2016021,KX2015013);山东省中医药科技发展计划项目(2015-239);山东省医药卫生科技发展计划项目资助(2016WS0667)

Abstract: Objective: To investigate the effects of endothelial progenitor cells (EPCs) under shear stress on the biological function such as proliferation, adhesion, migration, apoptosis and expression of α-smooth muscle actin (α-SMA), collagen-I and collagen-Ⅲ of hepatic stellate cells (HSCs). Methods: HSCs and EPCs were inoculated into the upper and lower layers of the co-culture chamber respectively and co-incubated for 24 hours. Then, 12 dyne/cm2 shear stress was applied to EPCs cells for another 24 hours. After that, proliferation, adhesion, migration and apoptosis of HSCs were detected by cell counting kit-8 (CCK-8) kit, cell adherent assay, Boyden cell migration assay and flow cytometry respectively. Fluorescence quantitative PCR and Western blot were used to detect the mRNA and protein expression of alpha -SMA, collagen I and collagen-Ⅲ in HSCs. Results: Under shear stress, EPCs ecological niche could obviously inhibit the proliferation, adhesion and migration of HSCs, promote the apoptosis of HSCs, and down-regulate the mRNA and protein expression of collagen-I, collagen-Ⅲ in HSC cells. Conclusion: Under shear stress, EPCs ecological niche could inhibit the fibrosis development of HSCs to a certain extent.

Key words: endothelial progenitor cells, hepatic stellate cells, shear stress, co-culture, ecological niche

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