Effects of miRNA-1,miRNA-21 in plasma on in-stent restenosis in patients with coronary heart disease and diabetes mellitus after percutaneous coronary intervention

doi:10.12047/j.cjap.5643.2018.070

Abstract

Abstract: Objective: To observe the expression differences of the plasma miRNA-1, miRNA-21 between patients with coronary heart disease (CHD) and without coronary artery lesions, between patients with in-stent restenosis (ISR) and none in-stent restenosis (NISR), and to study their predictive value for ISR occurred after percutaneous coronary intervention (PCI) in patients with CHD and diabetes mellitus (DM). Methods: The selected subjects were divided into CHD group in which patients were implemented stenting (n=187), and control group in which patients were without coronary artery lesions (n=195). According to the guidelines, the control group was divided into normal group (n=150), simple-DM group (n=45); the CHD group was divided into simple-CHD group (n=119) and CHD-DM group (n=68), the CHD group was also divided into ISR group (n=48), NISR group (n=139), and the ISR group was divided into simple-ISR group (n=26) and ISR-DM group (n=22) again. Plasma was collected from each group, and total RNA was extracted, the level of blood miRNA-1, miRNA-21 of each group was detected, and their level differences were analyzed. Results: Compared with control group, the level of miRNA-1 and miRNA-21 of CHD group was increased (P<0.05); compared with NISR group, the level of miRNA-1 and miRNA-21 of ISR group was increased (P<0.05). The incidence of ISR of CHD-DM group was obviously higher than that of simple-CHD group, ISR-DM group's level of miRNA-21 was higher than that of simple-ISR group (P<0.05), and there was no difference of miRNA-1 level between ISR and ISR-DM group (P<0.05). In Logistics, for CHD patents, the OR of DM, miRNA-1, miRNA-21 were 2.132, 3.066, 1.924 respectively (P<0.05); for CHD patents with ISR, the OR of DM, miRNA-21 were 2.123, 3.066 respectively (P<0.05); especially for CHD and DM patents with ISR, the OR of miRNA-21 was 9.148 (P<0.05). In ROC curve, for CHD patients with ISR, the AUC of miRNA-1, miRNA-21 were 0.854, 0.857 respectively; for CHD-DM patients with ISR, the AUC of miRNA-21 was 0.783. Conclusion: To predict the occurrence of ISR for CHD patients, the plasma miRNA-1 and miRNA-21 have a relatively high specificity and sensitivity, for CHD patients with DM, miRNA-21 may have a higher clinical value.

Key words: miRNA-1, miRNA-21, coronary heart disease, in-stent restenosis, diabetes mellitus

GUAN Jing-jing, ZHANG Ying, LIU Yu-jie. Effects of miRNA-1,miRNA-21 in plasma on in-stent restenosis in patients with coronary heart disease and diabetes mellitus after percutaneous coronary intervention[J]. CJAP, 2018, 34(4): 304-308.

References

[1] Miglionico M, Mangiacapra F, Nusca A, et al. Efficacy and safety of paclitaxel coated balloon for the treatment of in-stent restenosisin high-risk patients[J]. Am J Cardiol, 2015, 116(11):1690-1694.
[2] Bernardo BC, Ooi JY, Lin RC, et al. miRNA therapeu-tics:a new class of drugs with potential therapeutic appli-cations in the hear[J]. Future Med Chem, 2015, 7(13):1771-1792.
[3] Sluijter JP, van Mil A, van Vliet P, et al. MicroRNA-1 and-499regulate differentiation and proliferation in hu-man-derived cardiomyocyte progenitor cells[J]. Arterio-scler Thromb Vasc Biol, 2010, 30:859-868.
[4] Maeng M, Baranauskas A, Christiansen EH, et al. A 10-month angiographic and 4-year clinical outcome of everolimus-eluting versussirolimus-eluting coronary stents in patients with diabetes mellitus[J]. Catheter Cardiovasc Interv, 2015, 86(7):1161-1167.
[5] Jeff Bruce, Angela B, Wei Shi, et al. Identification of a microRNA signature associated with risk of distant metas-tasis in nasopharyngeal carcinoma[J]. Oncotarget, 2015, 6(6):4537-4550.
[6] Gui YJ, Yang T, Liu Q, et al. Soluble epoxide hydrolase inhibitors, t-AUCB, regulated microRNA-1and its target genes in myocardial infarction mice[J]. Oncotarget, 2017, 8(55):94635-94649.
[7] Wei Y, Zhu M, Corbalan-Campos J, et al. Regulation of Csflr and Bc16 in macrophages mediates the stage-specific effects of microRNA-155on atherosclerosis[J]. Arterio-scler Thromb Vasc Biol, 2015, 35(4):796-803.
[8] Mehran R, Dangas G, Abizaid AS, et al. Angiographic pauem of in-stent restenosis:classification and implica-tion for long terra outcome[J]. Circulation, 1999, 100(18):1872-1878.
[9] Zhu HQ, Wang F, Dong LY, et al. MicroRNA-1modu-lates oxLDL-induced hyperlipidemia by down-regulating MLCK and ERK/p38MAPK pathway[J]. Life Sci, 2014, 107(1-2):21-26.
[10] Xu C, Lu Y, Pan Z, et al. The muscle-specific microR-NAs miR-1and miR-133produce opposing effects on ap-optosis by targeting HSP60, HSP70 and caspase-9in car-diomyocytes[J]. Cell Sci, 2007, 120(17):3045-3052.
[11] Zhou Q, Sun Q, Zhang Y, et al. Up-regulation of miR-NA-21expression promotes migration and proliferation of sca-1⁺cardiac stem cells in mice[J]. Med Sci Monit, 2016, 23(22):1724-1732.
[12] Jansen F, Sch fer L, Wang H, et al. Kinetics of circulat-ing microRNAs in response to cardiac stress in patients with coronary artery disease[J]. Oncotarget, 2017, 8(55):94635-94649.
[13] Erkan AF, Tanindi A, Kocaman SA, et al. Epicardial adipose tissue thickness is an independent predictor of critical and complex coronary artery disease by gensini and syntax scores[J]. Tex Heart Inst J, 2016, 43(1):29-37.
[14] 李雯琦, 刘书雷, 徐明彤, 等. miRNA-1与糖尿病心肌病心肌细胞肥大相关性的初步探讨[J]. 中山大学学报(医学科学版), 2013, 34(2):170-174.
[15] 刘显庆, 姜德谦, 罗玉梅, 等. 葡萄糖和胰岛素对血管内皮细胞中microRNA-21的表达影响[J]. 中南大学心血管病学, 2010, 21(6):1076-1079.
[16] 张成, 章少中, 张亚洲, 等. 诱导心脏肌成纤维细胞向心肌样细胞转分化的miRNA[J]. 中国组织工程研究, 2013, 1(45):7924-7931.
[17] Nakatani T, Chen T, Partridge NC, et al. MMP-13is one of the critical mediators of the effect of HDAC4 dele-tion on the skeleton[J]. Bone, 2016, 90(9):142-151.
[18] 剌梅, 杨登魁, 李江, 等. 血清miRNA-1、miRNA-21检测预测PCI术后患者再狭窄的临床价值[J]. 海南医学, 2015, 28(6):923-927.
[19] Feng B, Cao Y, Chen S, et al. miRNA-1regulates endo-thelin-1in diabetes[J]. Life Sci, 2014, 98(1):18-23.
[20] Ji R, Cheng Y, Yue J, et al. miRNA expression signa-ture and antisense mediated depletion reveal an essential role of miRNA invascular neointimal lesion formation[J]. Circ Res, 2007, 100(11):1579-1588.