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CJAP ›› 2018, Vol. 34 ›› Issue (5): 414-417.doi: 10.12047/j.cjap.5660.2018.094

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Effects of astragaloside-IV on the expression of inflammatory factor and proliferation of glomerular mesangial cells induced by angiotensin Ⅱ

XIONG Li1, LYU Meng-juan2, DOU De-yu2, MA Yu-hong1   

  1. 1. Department of Diagnostic, Wannan Medical College, Wuhu 241001, China;
    2. Department of Central Lab, Wannan Medical College, Wuhu 241001, China
  • Received:2017-12-13 Revised:2018-09-25 Online:2018-09-28 Published:2019-02-21
  • Supported by:
    安徽省自然科学基金(1508085SMH236);2016安徽省高校优秀人才引进和培育计划国外访学重点项目(gxfxZD2016162);2016年皖南医学院校青年基金(WK201608)

Abstract: Objective: To study the effects of astragaloside-IV (As-IV) on the expression of inflammatory factor and proliferation of glomerular mesangial cells (GMCs) induced by angiotensin Ⅱ(AngⅡ). Methods: The in vitro model of diabetic nephropathy(DN) was mimic by angiotensin Ⅱ (10-6mol/L)inducing GMCs injury. Then the GMCs were treated with As-IV at different concentrations(25,50,100 μmol/L)for 48 hours. The proliferation of GMCs was detected by MTT. The level of reactive oxidative species (ROS) was measured by flow cytometry. The expression of monocyte chemoattractant protein-1(MCP-1) protein in supernatant was detected by enzyme- linked immunosorbent assay (ELISA). The expression of transforming growth factor-β1(TGF-β1) in GMCs was measured by Western blot. Results: Compared with model group, the proliferation of GMCs was inhibited in As-IV group. As-IV decreased the level of intercellular ROS, down-regulated the secretion of MCP-1 and the expression of TGF-β1 proteins. Conclusion: As-IV could inhibit cell proliferation and inflammatory factors expression on GMCs induced by AngⅡ.

Key words: rat glomerular mesangial cells, astragaloside-IV, reactive oxygen species, monocyte chemoattractant protein-1

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