Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism

doi:10.12047/j.cjap.5663.2018.063

CJAP ›› 2018, Vol. 34 ›› Issue (3): 268-272.doi: 10.12047/j.cjap.5663.2018.063

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Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism

ZHANG Yan-zi^1,2, KE Rui-jun², JIANG Pan-ruo², YANG Lin-jun³, CHEN Jia-yu^1,4

1. School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035;
2. Medical School, Shaoxing University, Shaoxing 312000;
3. Taizhou Municipal Hospital, Taizhou 318000;
4. Taizhou University Medical School, Taizhou 318000, China

Received:2017-12-28 Revised:2018-02-23 Online:2018-05-28 Published:2018-09-08
Supported by:
国家自然科学基金资助项目（81402979）；浙江省科技厅资助项目（2015C33270）；浙江省卫生厅资助项目（2014KYA230）

Abstract

Abstract: Objective: To investigate the effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its related mechanism.Methods: Gastric cancer cell lines HGC-27 and SGC-7901 were treated with 16, 8, 4, 2, 1 and 0 μg/ml white lentil polysaccharides for 24, 48 and 72 hours respectively, each experiment 3 wells. The proliferation activities of gastric cancer cells were detected with CellTiter 96^® AQueous Non-Radioactive Cell Proliferation Assay (MTS). HGC-27 and SGC-7901 cells were treated with 0 or 4 μg/ml white lentil polysaccharides for 24 hours respectively, each experiment 3 wells, then the changes of cell mitochondrial membrane potential were observed by JC-1 staining, cell cycle and cell apoptosis rates were evaluated with flow cytometry, the levels of mRNA expression of Bcl-2, caspase-3 and Bax genes were investigated with real-time quantitative PCR detecting system (QPCR).Results: After the gastric cancer cell lines HGC-27 and SGC-7901 were treated with white lentil polysaccharides, the mitochondrial membrane potential of the cells was significantly reduced, proliferation activities of the cells were inhibited obviously (P<0.01), and the inhibition degree was related with drug concentration and incubation time. Flow cytometry analysis showed that the apoptosis rates of HGC-27 and SGC-7901 cell lines were 53.15% and 38.77% respectively, which were significantly increased compared with PBS treatment groups (8.07% and 6.03%, P<0.01). And the cell cycle of these cells was not influenced significantly. The results of QPCR showed that Bcl-2 gene transcription level of HGC-27 and SGC-7901 cell lines was significantly down-regulated, and the transcription levels of Bax and caspase-3 genes were significantly increased (P<0.01).Conclusion: The white lentil polysaccharides can induce the apoptosis of gastric cancer cells lines HGC-27 and SGC-7901, and the mechanism of their apoptosis may be mediated by the regulation of Bax-Bcl-2-caspase3 pathway.

Key words: white lentil polysaccharides, cell apoptosis, human gastric cancer cell line HGC-27, human gastric cancer cell line SGC-7901

ZHANG Yan-zi, KE Rui-jun, JIANG Pan-ruo, YANG Lin-jun, CHEN Jia-yu. Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism[J]. CJAP, 2018, 34(3): 268-272.

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Effect of white lentil polysaccharides on the apoptosis of human gastric cancer cells and its mechanism

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