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CJAP ›› 2018, Vol. 34 ›› Issue (5): 418-421.doi: 10.12047/j.cjap.5667.2018.095

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Effects of laser stimulation with different power on oxygen consumption rate of C2C12 myoblasts in mice

FENG Yu, CHENG Ze-peng, SUN Peng, SHI Reng-fei   

  1. Academy of Sport Sciences, Shanghai University of Sport, Shanghai 200438, China
  • Received:2018-01-03 Revised:2018-09-25 Online:2018-09-28 Published:2019-02-21
  • Contact: 史仍飞,Tel:021-65507363;E-mail:rfshi@sus.edu.cn E-mail:rfshi@sus.edu.cn
  • Supported by:

Abstract: Objective: To investigate the effects of 650 nm laser irradiation on cell oxygen consumption rate in C2C12 myoblasts following different doses. Methods: C2C12 cells were irradiated with 650 nm laser(λ=650 nm, p=5 mW) with energy densities of 0, 0.4, and 0.8 J/cm2. Cell oxidative function was measured by oxygen consumption rate kit. Protein expression of myogenic determination factor (MyoD), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mammalian target of rapamycin (mTOR) and its phosphorylation were detected by Western blot. Results: Compared to the control group, the expression levels of MyoD, PGC-1α protein were increased and cell oxygen consumption rate was promoted in the low dose group(P<0.05). MyoD and PGC-1α protein expressions were also increased(P<0.05), the ratio of mTOR and its phosphorylationwere decreased significantly in the high dose group(P<0.05). Conclusion: 650 nm laser irradiation that dose is 0.4 J/cm2 enhances cell oxidative function, it related to that proper dose laser irradiation promoted the expression of PGC-1a protein.

Key words: laser irradiation, oxygen consumption rate, PGC-1α, C2C12 cells

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