Home  About Journal Instructions for Authors Editorial Board Subscribe Advertisement Messages Board Contact Us 中文

CJAP ›› 2018, Vol. 34 ›› Issue (5): 450-456.doi: 10.12047/j.cjap.5683.2018.102

Previous Articles     Next Articles

Study on 4 weeks medium intensity aeraobic exercise induce the difference expression of the proteomics in rat atrial muscle

PENG Yong1, SHI Shao-rong2, HUANG Si-min3, GUO Yuan-pan4, XU Zhe5, WAN Li-li6, LEI Xiong6   

  1. 1. Department of Health Sports Science, Nanjing Sport Institute, Nanjing 210014;
    2. College of Physical Education, Hunan Normal University, Changsha 410012;
    3. College of Physical Education and Health, Zhaoqing University, Zhaoqing 526061;
    4. Hunan College of Foreign Studies, Changsha 410116;
    5. Department of Physical Education, Hunan City University, Yiyang 413039;
    6. Department of Basic Medicine, Changsha Medical University, Changsha 410219, China
  • Received:2018-02-11 Revised:2018-09-25 Online:2018-09-28 Published:2019-02-21
  • Supported by:
    湖南省科技厅计划项目(2007FJ3075);江苏省教育厅高校自然科学研究面上项目(14KJB310002);江苏高校运动人体科学品牌专业建设工程资助项目(TAPP)

Abstract: Objective: To investigate the effect of moderate-intensity aerobic exercise on the differential expression of rat atrial muscle Proteomics and genes, which provide research basis for the rehabilitation of chronic cardiovascular diseases and exercise -induced cardiac remodeling research. Methods: Twenty male SD rats were randomly divided into control group and experimental group (n=10) according to body weight. Rats in the experimental group were trained (6 days per week),which lasted for 4 weeks of moderate-intensity aerobic exercise at a rate of 24 m·min-1 for 40 min (load intensity equivalent to 60%~70% VO2max). The proteins were separated by two dimensional gel electrophoresis, and the tandem time-of- flight mass spectrometer technique was used to identify 13 candidate target protein spots. The expression levels of these 13 protein spots were up-regulated more than 5 times or down -regulated to below 1/5. The mRNA of six target proteins were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results: By software analysis, the experimental group compared with the control group, there were 8 protein points which their expression reduced more than 4/5 and 5 protein points up-regulated more than 5 times, 13 proteins were identified by mass spectrometry protein spots, the final identification results acquired 8 proteins and a unknown protein of molecular mass 54 KDa, such as:pyruvate dehydrogenase E1α1, mitochondrial aconitate hydratase, protein disulfide isomerase A3, methylmalonic acid semialdehyde dehydrogenase, mitochondrial dihydrolipoic acid dehydrogenase, isovaleryl coenzyme A dehydrogenase, glutathione synthetase, mitogen-activated protein kinase 3 and so on. Compared with the control group, the mRNA expression of methylmalonic acid semialdehyde dehydrogenase in the atrial muscle of rats was decreased after 4 weeks of moderate aerobic exercise (P<0.05), the mRNA expression levels of mitochondria Ⅱ lipoic acid dehydrogenase, protein disulfide isomerase A3, mitochondrial aconitate hydratase and glutathione synthetase were decreased (P>0.05); The mRNA expression level of isopentenyl-CoA dehydrogenase was increased (P>0.05). The results indicated that the mRNA expression level was not completely consistent with the changes in mass spectrometry identification results. Conclusion: The 4 weeks moderate-intensity aerobic exercise induced ignificant changes of rats atrial muscle protemics. The majority of the 13 identified target proteins in this experiment are energy metabolism enzymes. The majority of the expression of the target protein and the mRNA expression in the atrial muscle is inconsistent and different. Exercise may affect the regulation of gene transcription or downstream translation and modification of these target proteins, resulting in the change of differential expression.

Key words: proteomics, aerobic exercise, rat, myocardial energy metabolism, mass spectrometry 2-DE

Copyright © 2015 CJAP, All Rights Reserved.
Powered by Beijing Magtech Co. Ltd