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CJAP ›› 2019, Vol. 35 ›› Issue (1): 47-49.doi: 10.12047/j.cjap.5690.2019.012

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Protective effects of Shenmai injection on intestinal mucosal barrier function in severely scalded rats

YANG Chen1, SONG Yao-yao2, JI Yi-fa3△   

  1. 1. Department of Burn and Plastic Surgery, Special Medical Center of Chinese People’s Armed Police Force, Tianjin 300162;
    2. Department of Burn and Plastic Surgery, the Sixth Medical Center of PLA General Hospital, Beijing 100048;
    3. Department of Cosmetic Surgery, Beijing Fengtai Youanmen Hospital, Beijing 100069, China
  • Received:2018-04-02 Online:2019-01-28 Published:2019-06-27

Abstract: Objective:To investigate the protective effects of shen-mai injection on intestinal barrier function in the early stage of 30% Ⅲ° scald, and to provide experimental basis for the prevention and control of enterogenic infection. Methods: A total of 60 Wistar rats were randomly divided into 6 groups: normal control group (without treatment), model control group (with 30% total body surface area (TBSA) fully thickness burn on the back), hexadecadrol (5 mg/kg) group, Shenmai injection (5, 10, 15 mg/kg) groups, with 10 rats in each group. After burned by scald apparatus, rats in each group were treated with drugs immediately by intraperitoneal injection once a day. At 72 hours after burned, the levels of plasma endotoxin, diamine oxidase (DAO), tumor necrosis factor-alpha (TNF-α), interleukins-6(IL-6) in all rats were detected and the mesenteric lymph nodes, liver and spleen were homogenized to culture for bacteria. The change of secretory immunoglobulin A (sIgA) in intestinal mucosa was measured. Results: Compared with normal control group, bacterial translocation quantity in mesenteric lymph nodes(MLN), liver, and spleen, and the plasma levels of DAO, endotoxin, TNF-α, IL-6 and the level of sIgA in intestinal mucosa in model control group were increased significantly (P<0.01); compared with model control group, bacterial translocation quantity in MLN, liver, and spleen, and the plasma levels of DAO, endotoxin, TNF-α, IL-6 and the level of sIgA in intestinal mucosa in hexadecadrol (5 mg/kg) group and shen-mai injection (5, 10, 15 mg/kg) groups were decreased significantly (P<0.05 or P<0.01). Conclusion: Shen-mai injection can alleviate intestinal mucosa injury caused by severe scald, and the effects are similar with those of dexamethasone, and the effect is better in the high-dose group.

Key words: scald, intestinal mucosal barrier, bacterial translocation, endotoxin, sIgA, rats

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