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CJAP ›› 2019, Vol. 35 ›› Issue (1): 50-54.doi: 10.12047/j.cjap.5703.2019.013

• ORIGINAL ARTICLES • Previous Articles     Next Articles

Effects of ELK-1/JNK/c-Fos on apoptosis of rat hippocampal neurons cultured in vitro with Zuogui Jiangtang Jieyu Formula in simulated diabetes mellitus complicated with depression

LIU Zhuo1, LIU Jian2, LING Jia1, YANG Qin1, YANG Hui2, MENG Pan1, DU Qing3, ZHAO Hong-qing1, WANG Yu-hong1△   

  1. 1. Hunan University of Chinese Medicine, Hunan Key Laboratory of Chinese MateriaMedica Powder and Innovative Drugs Established by Provincial and Ministry, Changsha 410208;
    2. First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007;
    3. Hunan Academy of Chinese Medicine, Changsha 412000, China
  • Received:2018-05-02 Online:2019-01-28 Published:2019-06-27

Abstract: Objective:To study the effects of Zuogui Jiangtang Jieyu Formula (ZGJTJYF, the Chinese Medicine) on hippocampal neuron apoptosis in diabetes mellitus complicated with depression (DD). Methods: The primary cultured hippocampal neurons were treated with high glucose (150 mmol/L) and corticosterone (200 micromol/L) to establish the cell model of DD in vitro. The cultured hippocampal neurons were randomly divided into five groups: blank serum group, normal group, Zuogui Jiangtang Jieyu recipe drug-containing serum group, positive drug (metformin + fluoxetine) drug-containing serum group and model group (three compound holes in each group). The model group and the normal group were given the same amount of culture medium, and the other groups were given the corresponding serum with 10% volume fraction for 18 hours. Hoechst staining, high content cell imaging and RT-PCR were used to detect the apoptosis of hippocampal neurons and the expressions of apoptosis-related ETS-like 1 transcription factor(ELK-1), C-Jun N-terminal kinase(JNK) and c-Fos proteins and genes. Results: Compared with the blank group, the apoptotic number of hippocampal neurons in the model group was increased significantly, and the expression levels of ELK-1, JNK and c-Fos were increased significantly (P<0.05). Compared with the model group, the local bright spots of hippocampal neurons in the Zuogui Jiangtang Jieyu recipe-containing serum group and the positive drug-containing serum group were decreased significantly, and the number of apoptotic cells was decreased significantly. The expressions of JNK, c-fos protein and mRNA were down-regulated significantly (P< 0.05), and the neural network and dendritic junction were improved significantly. Conclusion: Zuo Gui Jiang Tang Jie Yu Formula can reverse the expressions of ELK-1, JNK and c-Fos signals in hippocampal neurons under DD environment and play an anti-apoptotic effect.

Key words: Zuogui Jiangtang Jieyu Formula, diabetes mellitus with depression, apoptosis pathway, hippocampal neurons, rats

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